Skip to main content
PMC home page
Biophysical Journal logoLink to Biophysical Journal
. 1965 Jul;5(4):487–509. doi: 10.1016/S0006-3495(65)86731-5

A Unified Kinetic Hypothesis of Carrier-Mediated Transport

Its Applications

M Silverman, C A Goresky
PMCID: PMC1367889  PMID: 5893156

Abstract

A model analysis of the process of carrier mediated membrane transport is presented, wherein the carrier is present in two forms of differing affinity for substrate. The two forms of carrier undergo interconversion by asymmetric metabolic reactions on each side of the membrane. From this model system expressions are derived for the steady-state distribution ratio for substrate, for the unidirectional fluxes of substrate and hence for the initial velocity of uptake of substrate, and for the effect of preloading cells upon the initial velocity of uptake of labeled substrate. These expressions are applied to published data for glycine transport in Ehrlich ascites tumor cells to obtain numerical values for the parameters of a concentrative membrane carrier system. Concentrative uptake is shown to be consequent to the differing affinities of the two forms of carrier. When the affinities of the two forms are equal, equilibrative uptake occurs. The model analysis is applied to the phenomena of metabolic and competitive inhibition.

Full text

PDF
488

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AKEDO H., CHRISTENSEN H. N. Nature of insulin action on amino acid uptake by the isolated diaphragm. J Biol Chem. 1962 Jan;237:118–122. [PubMed] [Google Scholar]
  2. CHRISTENSEN H. N., RIGGS T. R., FISCHER H., PALATINE I. M. Amino acid concentration by a free cell neoplasm; relations among amino acids. J Biol Chem. 1952 Sep;198(1):1–15. [PubMed] [Google Scholar]
  3. HEINZ E. Kinetic studies on the influx of glycine-1-C14 into the Ehrlich mouse ascites carcinoma cell. J Biol Chem. 1954 Dec;211(2):781–790. [PubMed] [Google Scholar]
  4. HEINZ E., MARIANI H. A. Concentration work and energy dissipation in active transport of glycine into carcinoma cells. J Biol Chem. 1957 Sep;228(1):97–111. [PubMed] [Google Scholar]
  5. HEINZ E. The exchangeability of glycine accumulated by carcinoma cells. J Biol Chem. 1957 Mar;225(1):305–315. [PubMed] [Google Scholar]
  6. HEINZ E., WALSH P. M. Exchange diffusion, transport, and intracellular level of amino acids in Ehrlich carcinoma cells. J Biol Chem. 1958 Dec;233(6):1488–1493. [PubMed] [Google Scholar]
  7. HORECKER B. L., THOMAS J., MONOD J. Galactose transport in Escherichia coli. I. General properties as studied in a galactokinaseless mutant. J Biol Chem. 1960 Jun;235:1580–1585. [PubMed] [Google Scholar]
  8. JACQUEZ J. A. Carrier-amino acid stoichiometry in amino acid transport in Ehrlich ascites cells. Biochim Biophys Acta. 1963 Apr;71:15–33. doi: 10.1016/0006-3002(63)90981-8. [DOI] [PubMed] [Google Scholar]
  9. JACQUEZ J. A. THE KINETICS OF CARRIER-MEDIATED TRANSPORT: STATIONARY-STATE APPROXIMATIONS. Biochim Biophys Acta. 1964 Mar 30;79:318–328. [PubMed] [Google Scholar]
  10. Jacquez J. A. THE KINETICS OF CARRIER-MEDIATED ACTIVE TRANSPORT OF AMINO ACIDS. Proc Natl Acad Sci U S A. 1961 Feb;47(2):153–163. doi: 10.1073/pnas.47.2.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. OXENDER D. L., CHRISTENSEN H. N. DISTINCT MEDIATING SYSTEMS FOR THE TRANSPORT OF NEUTRAL AMINO ACIDS BY THE EHRLICH CELL. J Biol Chem. 1963 Nov;238:3686–3699. [PubMed] [Google Scholar]
  12. PAINE C. M., HEINZ E. The structural specificity of the glycine transport system of Ehrlich carcinoma cells. J Biol Chem. 1960 Apr;235:1080–1085. [PubMed] [Google Scholar]
  13. ROSENBERG T., WILBRANDT W. Uphill transport induced by counterflow. J Gen Physiol. 1957 Nov 20;41(2):289–296. doi: 10.1085/jgp.41.2.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. ROTMAN B., RADOJKOVIC J. GALACTOSE TRANSPORT IN ESCHERICHIA COLI. THE MECHANISM UNDERLYING THE RETENTION OF INTRACELLULAR GALACTOSE. J Biol Chem. 1964 Oct;239:3153–3156. [PubMed] [Google Scholar]
  15. Rosenberg T., Wilbrandt W. Carrier transport uphill. I. General. J Theor Biol. 1963 Sep;5(2):288–305. doi: 10.1016/0022-5193(63)90065-1. [DOI] [PubMed] [Google Scholar]
  16. WILBRANDT W., ROSENBERG T. The concept of carrier transport and its corollaries in pharmacology. Pharmacol Rev. 1961 Jun;13:109–183. [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES