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. 1995 Sep;86(1):134–140.

Induction of mucosal immunity in cotton rats to haemagglutinin-esterase glycoprotein of bovine coronavirus by recombinant adenovirus.

M E Baca-Estrada 1, X Liang 1, L A Babiuk 1, D Yoo 1
PMCID: PMC1383821  PMID: 7590874

Abstract

An effective vaccine against enteric bovine coronavirus (BCV) must be able to induce mucosal immunity. We recently described the construction of recombinant human adenovirus type 5 (hAd5) carrying the BCV haemagglutinin-esterase (HE) gene in the early transcription region 3 of the adenovirus genome. In this study, we examined the induction of systemic and mucosal immune responses to the hAd5 vector carrying the BCV HE gene (AdBcHE) following intranasal or enteric immunization of cotton rats. Regardless of the route of administration, mucosal immunization with AdBcHE induced significant levels of anti-HE IgG antibodies in serum. In addition, following intranasal immunization with AdBcHE, significant levels of anti-HE IgA antibodies were found in lung washes of immunized cotton rats. Furthermore, the specific anti-HE antibodies in sera and mucosal secretions efficiently neutralized BCV infectivity in vitro. T-cell proliferation and cell-mediated cytotoxic responses against the BCV HE were elicited in the spleen of intranasally immunized animals. The results demonstrate that mucosal immunization with AdBcHE is capable of inducing both systemic and mucosal immunity to the BCV HE. These immune responses may be important in protecting animals from BCV infection.

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Selected References

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  1. Babiuk L. A., Sabara M., Hudson G. R. Rotavirus and coronavirus infections in animals. Prog Vet Microbiol Immunol. 1985;1:80–120. [PubMed] [Google Scholar]
  2. Callebaut P. E., Pensaert M. B. Characterization and isolation of structural polypeptides in haemagglutinating encephalomyelitis virus. J Gen Virol. 1980 May;48(1):193–204. doi: 10.1099/0022-1317-48-1-193. [DOI] [PubMed] [Google Scholar]
  3. Deregt D., Babiuk L. A. Monoclonal antibodies to bovine coronavirus: characteristics and topographical mapping of neutralizing epitopes on the E2 and E3 glycoproteins. Virology. 1987 Dec;161(2):410–420. doi: 10.1016/0042-6822(87)90134-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Deregt D., Gifford G. A., Ijaz M. K., Watts T. C., Gilchrist J. E., Haines D. M., Babiuk L. A. Monoclonal antibodies to bovine coronavirus glycoproteins E2 and E3: demonstration of in vivo virus-neutralizing activity. J Gen Virol. 1989 Apr;70(Pt 4):993–998. doi: 10.1099/0022-1317-70-4-993. [DOI] [PubMed] [Google Scholar]
  5. Deregt D., Sabara M., Babiuk L. A. Structural proteins of bovine coronavirus and their intracellular processing. J Gen Virol. 1987 Nov;68(Pt 11):2863–2877. doi: 10.1099/0022-1317-68-11-2863. [DOI] [PubMed] [Google Scholar]
  6. Gallichan W. S., Johnson D. C., Graham F. L., Rosenthal K. L. Mucosal immunity and protection after intranasal immunization with recombinant adenovirus expressing herpes simplex virus glycoprotein B. J Infect Dis. 1993 Sep;168(3):622–629. doi: 10.1093/infdis/168.3.622. [DOI] [PubMed] [Google Scholar]
  7. Hanke T., Graham F. L., Rosenthal K. L., Johnson D. C. Identification of an immunodominant cytotoxic T-lymphocyte recognition site in glycoprotein B of herpes simplex virus by using recombinant adenovirus vectors and synthetic peptides. J Virol. 1991 Mar;65(3):1177–1186. doi: 10.1128/jvi.65.3.1177-1186.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hogue B. G., Brian D. A. Structural proteins of human respiratory coronavirus OC43. Virus Res. 1986 Aug;5(2-3):131–144. doi: 10.1016/0168-1702(86)90013-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. King B., Brian D. A. Bovine coronavirus structural proteins. J Virol. 1982 May;42(2):700–707. doi: 10.1128/jvi.42.2.700-707.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Luytjes W., Bredenbeek P. J., Noten A. F., Horzinek M. C., Spaan W. J. Sequence of mouse hepatitis virus A59 mRNA 2: indications for RNA recombination between coronaviruses and influenza C virus. Virology. 1988 Oct;166(2):415–422. doi: 10.1016/0042-6822(88)90512-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Morin J. E., Lubeck M. D., Barton J. E., Conley A. J., Davis A. R., Hung P. P. Recombinant adenovirus induces antibody response to hepatitis B virus surface antigen in hamsters. Proc Natl Acad Sci U S A. 1987 Jul;84(13):4626–4630. doi: 10.1073/pnas.84.13.4626. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Murphy B. R., Collins P. L., Lawrence L., Zubak J., Chanock R. M., Prince G. A. Immunosuppression of the antibody response to respiratory syncytial virus (RSV) by pre-existing serum antibodies: partial prevention by topical infection of the respiratory tract with vaccinia virus-RSV recombinants. J Gen Virol. 1989 Aug;70(Pt 8):2185–2190. doi: 10.1099/0022-1317-70-8-2185. [DOI] [PubMed] [Google Scholar]
  13. Nedrud J. G., Liang X. P., Hague N., Lamm M. E. Combined oral/nasal immunization protects mice from Sendai virus infection. J Immunol. 1987 Nov 15;139(10):3484–3492. [PubMed] [Google Scholar]
  14. Oualikene W., Gonin P., Eloit M. Short and long term dissemination of deletion mutants of adenovirus in permissive (cotton rat) and non-permissive (mouse) species. J Gen Virol. 1994 Oct;75(Pt 10):2765–2768. doi: 10.1099/0022-1317-75-10-2765. [DOI] [PubMed] [Google Scholar]
  15. Prevec L., Campbell J. B., Christie B. S., Belbeck L., Graham F. L. A recombinant human adenovirus vaccine against rabies. J Infect Dis. 1990 Jan;161(1):27–30. doi: 10.1093/infdis/161.1.27. [DOI] [PubMed] [Google Scholar]
  16. Prevec L., Schneider M., Rosenthal K. L., Belbeck L. W., Derbyshire J. B., Graham F. L. Use of human adenovirus-based vectors for antigen expression in animals. J Gen Virol. 1989 Feb;70(Pt 2):429–434. doi: 10.1099/0022-1317-70-2-429. [DOI] [PubMed] [Google Scholar]
  17. Prince G. A., Porter D. D., Jenson A. B., Horswood R. L., Chanock R. M., Ginsberg H. S. Pathogenesis of adenovirus type 5 pneumonia in cotton rats (Sigmodon hispidus). J Virol. 1993 Jan;67(1):101–111. doi: 10.1128/jvi.67.1.101-111.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ramsay A. J., Ruby J., Ramshaw I. A. A case for cytokines as effector molecules in the resolution of virus infection. Immunol Today. 1993 Apr;14(4):155–157. doi: 10.1016/0167-5699(93)90277-R. [DOI] [PubMed] [Google Scholar]
  19. Rosenfeld M. A., Siegfried W., Yoshimura K., Yoneyama K., Fukayama M., Stier L. E., Päkkö P. K., Gilardi P., Stratford-Perricaudet L. D., Perricaudet M. Adenovirus-mediated transfer of a recombinant alpha 1-antitrypsin gene to the lung epithelium in vivo. Science. 1991 Apr 19;252(5004):431–434. doi: 10.1126/science.2017680. [DOI] [PubMed] [Google Scholar]
  20. Rosenfeld M. A., Yoshimura K., Trapnell B. C., Yoneyama K., Rosenthal E. R., Dalemans W., Fukayama M., Bargon J., Stier L. E., Stratford-Perricaudet L. In vivo transfer of the human cystic fibrosis transmembrane conductance regulator gene to the airway epithelium. Cell. 1992 Jan 10;68(1):143–155. doi: 10.1016/0092-8674(92)90213-v. [DOI] [PubMed] [Google Scholar]
  21. Vlasak R., Luytjes W., Leider J., Spaan W., Palese P. The E3 protein of bovine coronavirus is a receptor-destroying enzyme with acetylesterase activity. J Virol. 1988 Dec;62(12):4686–4690. doi: 10.1128/jvi.62.12.4686-4690.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Waltner-Toews D., Martin S. W., Meek A. H., McMillan I., Crouch C. F. A field trial to evaluate the efficacy of a combined rotavirus-coronavirus/Escherichia coli vaccine in dairy cattle. Can J Comp Med. 1985 Jan;49(1):1–9. [PMC free article] [PubMed] [Google Scholar]
  23. Yoo D., Graham F. L., Prevec L., Parker M. D., Benkö M., Zamb T., Babiuk L. A. Synthesis and processing of the haemagglutinin-esterase glycoprotein of bovine coronavirus encoded in the E3 region of adenovirus. J Gen Virol. 1992 Oct;73(Pt 10):2591–2600. doi: 10.1099/0022-1317-73-10-2591. [DOI] [PubMed] [Google Scholar]
  24. Zhang X. M., Kousoulas K. G., Storz J. The hemagglutinin/esterase gene of human coronavirus strain OC43: phylogenetic relationships to bovine and murine coronaviruses and influenza C virus. Virology. 1992 Jan;186(1):318–323. doi: 10.1016/0042-6822(92)90089-8. [DOI] [PMC free article] [PubMed] [Google Scholar]

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