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. 1991 Mar;72(3):344–349.

IL-4 suppresses IL-1 beta, TNF-alpha and PGE2 production by human peritoneal macrophages.

P H Hart 1, R L Cooper 1, J J Finlay-Jones 1
PMCID: PMC1384393  PMID: 2026443

Abstract

Human interleukin-4 (IL-4) down-regulates IL-1 and tumour necrosis factor-alpha (TNF-alpha) production by monocytes stimulated in vitro. In contrast, in studies of activation of murine macrophages, both stimulatory and inhibitory functions of murine IL-4 have been documented. To investigate whether opposing activities of IL-4 reflect a difference in the target cell studied, due either to cell maturation or the site from which the cells were isolated, we examined the effect of IL-4 on human peritoneal macrophage production of IL-1 beta, TNF-alpha and prostaglandin E2 (PGE2). Human peritoneal macrophages stimulated with lipopolysaccharide (LPS) produced levels of these mediators that were at least as great as those previously reported for monocytes. Similarly, IL-4 was inhibitory for peritoneal macrophage mediator production after in vitro stimulation. Thus, IL-4 has effects on human peritoneal macrophages similar to those on blood monocytes. In addition, as it down-regulates mediator production by cells that have left the circulation, it may be important in controlling the immune response in tissues.

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Selected References

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  1. Abramson S. L., Gallin J. I. IL-4 inhibits superoxide production by human mononuclear phagocytes. J Immunol. 1990 Jan 15;144(2):625–630. [PubMed] [Google Scholar]
  2. Becker S., Johnson C., Halme J., Haskill S. Interleukin-1 production and antigen presentation by normal human peritoneal macrophages. Cell Immunol. 1986 Apr 1;98(2):467–476. doi: 10.1016/0008-8749(86)90305-9. [DOI] [PubMed] [Google Scholar]
  3. Cichocki T., Hanicki Z., Sułowicz W., Smoleński O., Kopeć J., Zembala M. Output of peritoneal cells into peritoneal dialysate. Cytochemical and functional studies. Nephron. 1983;35(3):175–182. doi: 10.1159/000183070. [DOI] [PubMed] [Google Scholar]
  4. Crawford R. M., Finbloom D. S., Ohara J., Paul W. E., Meltzer M. S. B cell stimulatory factor-1 (interleukin 4) activates macrophages for increased tumoricidal activity and expression of Ia antigens. J Immunol. 1987 Jul 1;139(1):135–141. [PubMed] [Google Scholar]
  5. Davies S. J., Suassuna J., Ogg C. S., Cameron J. S. Activation of immunocompetent cells in the peritoneum of patients treated with CAPD. Kidney Int. 1989 Oct;36(4):661–668. doi: 10.1038/ki.1989.243. [DOI] [PubMed] [Google Scholar]
  6. Donnelly R. P., Fenton M. J., Finbloom D. S., Gerrard T. L. Differential regulation of IL-1 production in human monocytes by IFN-gamma and IL-4. J Immunol. 1990 Jul 15;145(2):569–575. [PubMed] [Google Scholar]
  7. Essner R., Rhoades K., McBride W. H., Morton D. L., Economou J. S. IL-4 down-regulates IL-1 and TNF gene expression in human monocytes. J Immunol. 1989 Jun 1;142(11):3857–3861. [PubMed] [Google Scholar]
  8. Han J., Brown T., Beutler B. Endotoxin-responsive sequences control cachectin/tumor necrosis factor biosynthesis at the translational level. J Exp Med. 1990 Feb 1;171(2):465–475. doi: 10.1084/jem.171.2.465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hancock W. W., Zola H., Atkins R. C. Antigenic heterogeneity of human mononuclear phagocytes: immunohistologic analysis using monoclonal antibodies. Blood. 1983 Dec;62(6):1271–1279. [PubMed] [Google Scholar]
  10. Hart P. H., Vitti G. F., Burgess D. R., Whitty G. A., Piccoli D. S., Hamilton J. A. Potential antiinflammatory effects of interleukin 4: suppression of human monocyte tumor necrosis factor alpha, interleukin 1, and prostaglandin E2. Proc Natl Acad Sci U S A. 1989 May;86(10):3803–3807. doi: 10.1073/pnas.86.10.3803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hart P. H., Whitty G. A., Burgess D. R., Croatto M., Hamilton J. A. Augmentation of glucocorticoid action on human monocytes by interleukin-4. Lymphokine Res. 1990 Summer;9(2):147–153. [PubMed] [Google Scholar]
  12. Hart P. H., Whitty G. A., Piccoli D. S., Hamilton J. A. Synergistic activation of human monocytes by granulocyte-macrophage colony-stimulating factor and IFN-gamma. Increased TNF-alpha but not IL-1 activity. J Immunol. 1988 Sep 1;141(5):1516–1521. [PubMed] [Google Scholar]
  13. Heinzel F. P., Sadick M. D., Holaday B. J., Coffman R. L., Locksley R. M. Reciprocal expression of interferon gamma or interleukin 4 during the resolution or progression of murine leishmaniasis. Evidence for expansion of distinct helper T cell subsets. J Exp Med. 1989 Jan 1;169(1):59–72. doi: 10.1084/jem.169.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hudson M. M., Markowitz A. B., Gutterman J. U., Knowles R. D., Snyder J. S., Kleinerman E. S. Effect of recombinant human interleukin 4 on human monocyte activity. Cancer Res. 1990 Jun 1;50(11):3154–3158. [PubMed] [Google Scholar]
  15. Kenney J. S., Masada M. P., Eugui E. M., Delustro B. M., Mulkins M. A., Allison A. C. Monoclonal antibodies to human recombinant interleukin 1 (IL 1)beta: quantitation of IL 1 beta and inhibition of biological activity. J Immunol. 1987 Jun 15;138(12):4236–4242. [PubMed] [Google Scholar]
  16. Littman B. H., Dastvan F. F., Carlson P. L., Sanders K. M. Regulation of monocyte/macrophage C2 production and HLA-DR expression by IL-4 (BSF-1) and IFN-gamma. J Immunol. 1989 Jan 15;142(2):520–525. [PubMed] [Google Scholar]
  17. McBride W. H., Economou J. S., Nayersina R., Comora S., Essner R. Influences of interleukins 2 and 4 on tumor necrosis factor production by murine mononuclear phagocytes. Cancer Res. 1990 May 15;50(10):2949–2952. [PubMed] [Google Scholar]
  18. McLaughlin P. J., Elwood N. J., Ramadi L. T., Pica M. R., McKenzie I. F. Improvement in sensitivity of enzyme-linked immunosorbent assay for tumour necrosis factor. Immunol Cell Biol. 1990 Feb;68(Pt 1):51–55. doi: 10.1038/icb.1990.7. [DOI] [PubMed] [Google Scholar]
  19. Mizel S. B. The interleukins. FASEB J. 1989 Oct;3(12):2379–2388. doi: 10.1096/fasebj.3.12.2676681. [DOI] [PubMed] [Google Scholar]
  20. Mulé J. J., Smith C. A., Rosenberg S. A. Interleukin 4 (B cell stimulatory factor 1) can mediate the induction of lymphokine-activated killer cell activity directed against fresh tumor cells. J Exp Med. 1987 Sep 1;166(3):792–797. doi: 10.1084/jem.166.3.792. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Paul W. E., Ohara J. B-cell stimulatory factor-1/interleukin 4. Annu Rev Immunol. 1987;5:429–459. doi: 10.1146/annurev.iy.05.040187.002241. [DOI] [PubMed] [Google Scholar]
  22. Phillips W. A., Croatto M., Hamilton J. A. Priming the macrophage respiratory burst with IL-4: enhancement with TNF-alpha but inhibition by IFN-gamma. Immunology. 1990 Aug;70(4):498–503. [PMC free article] [PubMed] [Google Scholar]
  23. Stuart P. M., Zlotnik A., Woodward J. G. Induction of class I and class II MHC antigen expression on murine bone marrow-derived macrophages by IL-4 (B cell stimulatory factor 1). J Immunol. 1988 Mar 1;140(5):1542–1547. [PubMed] [Google Scholar]
  24. Tepper R. I., Pattengale P. K., Leder P. Murine interleukin-4 displays potent anti-tumor activity in vivo. Cell. 1989 May 5;57(3):503–512. doi: 10.1016/0092-8674(89)90925-2. [DOI] [PubMed] [Google Scholar]
  25. Thomassen M. J., Barna B. P., Rankin D., Wiedemann H. P., Ahmad M. Differential effect of recombinant granulocyte macrophage colony-stimulating factor on human monocytes and alveolar macrophages. Cancer Res. 1989 Aug 1;49(15):4086–4089. [PubMed] [Google Scholar]
  26. Verbrugh H. A., Keane W. F., Hoidal J. R., Freiberg M. R., Elliott G. R., Peterson P. K. Peritoneal macrophages and opsonins: antibacterial defense in patients undergoing chronic peritoneal dialysis. J Infect Dis. 1983 Jun;147(6):1018–1029. doi: 10.1093/infdis/147.6.1018. [DOI] [PubMed] [Google Scholar]
  27. Wewers M. D., Herzyk D. J. Alveolar macrophages differ from blood monocytes in human IL-1 beta release. Quantitation by enzyme-linked immunoassay. J Immunol. 1989 Sep 1;143(5):1635–1641. [PubMed] [Google Scholar]
  28. Widmer M. B., Acres R. B., Sassenfeld H. M., Grabstein K. H. Regulation of cytolytic cell populations from human peripheral blood by B cell stimulatory factor 1 (interleukin 4). J Exp Med. 1987 Nov 1;166(5):1447–1455. doi: 10.1084/jem.166.5.1447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wirth J. J., Kierszenbaum F., Zlotnik A. Effects of IL-4 on macrophage functions: increased uptake and killing of a protozoan parasite (Trypanosoma cruzi). Immunology. 1989 Feb;66(2):296–301. [PMC free article] [PubMed] [Google Scholar]
  30. te Velde A. A., Klomp J. P., Yard B. A., de Vries J. E., Figdor C. G. Modulation of phenotypic and functional properties of human peripheral blood monocytes by IL-4. J Immunol. 1988 Mar 1;140(5):1548–1554. [PubMed] [Google Scholar]

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