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. 1991 Mar;72(3):434–439.

Heterogeneity of murine bone marrow-derived mast cells: analysis of their proteinase content.

G F Newlands 1, D A Lammas 1, J F Huntley 1, A MacKellar 1, D Wakelin 1, H R Miller 1
PMCID: PMC1384407  PMID: 2026450

Abstract

The expression of granule proteinases by murine bone marrow-derived mast cells (BMMC) grown in vitro was compared with that of serosal mast cells (SMC) from the peritoneal cavity. The granules in a proportion of BMMC (0.4-13%) and in all SMC were labelled with fluorescent antibodies against rat mast cell protease I (RMCP I). The granules of 1-47% of BMMC and 100% of SMC were labelled with antibodies against a 30,000 molecular weight (MW) murine intestinal mast cell proteinase (MIMCP). Four antigens from BMMC, ranging in MW from 28,000 to 32,000 and a single 28,000 antigen from SMC were detected on Western blot using anti-MIMCP antibodies. Only the 28,000 MW antigens from BMMC and SMC were visualized in blots probed with anti-RMCP I. BMMC grown in the presence of conditioned medium from activated splenocytes or from the WEHI-3B myelomonocytic cell line contained 52-118 ng and 3-25 ng MIMCP/10(6) cells respectively, whereas SMC lacked detectable MIMCP. The selective labelling of the 28,000 MW antigens in BMMC and SMC with anti-RMCP I and the variable expression of this antigen in BMMC as detected by immunofluorescence indicates that BMMC are not a homogeneous population of cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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