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. 1991 Jul;73(3):334–341.

T-cell receptor-bearing cells from athymic nude rats respond to alloantigen in vitro but are defective in vivo.

S R Sarawar 1, C P Yang 1, E B Bell 1
PMCID: PMC1384552  PMID: 1831792

Abstract

T-like cells from congenitally athymic nude rats of the PVG (RT1c) strain were characterized both phenotypically and functionally. There was an age-dependent increase in the number of alpha beta TcR+CD3+ cells in the lymph nodes, spleen and thoracic duct of nude rats. These cells, which comprised up to 25% of lymph node cells in animals of 8-12 months in age, were also CD3+CD5+Thy-1.1-. The expression of CD4 and CD8 on T-like cells was mutually exclusive. Approximately 30% of the CD4+ cells expressed CD45RB and 50% of the TcR+ cells expressed RT6. B-cell-depleted TcR+ cells from nude animals gave proliferative responses to mitogenic lectins or immobilized anti-CD3 antibody. T-like cells showed comparable alloreactivity to their euthymic counterparts in mixed lymphocyte reactions (MLR) against three different MHC haplotypes and to lymphocytes of a congenic strain differing only in MHC-encoded products. Monoclonal antibodies (mAb) to CD4, MHC class II, alpha beta TcR and CD3 blocked the MLR. However, T-like cells failed to induce rejection of skin allografts of the same MHC haplotypes when adoptively transferred to athymic nude hosts and were unable to mount a normal graft-versus-host (GVH) response. These results indicate that lymphocytes may rearrange and express a functional TcR in the absence of a thymus, but that the thymic microenvironment is essential for T cells to acquire full in vivo function.

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Selected References

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