Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1988 Sep;65(1):53–58.

Detection and characterization of IgE-binding factors (IgE-BF) within supernatants of the cell line RPMI-8866, normal human sera and sera from atopic patients.

J Bujanowski-Weber 1, I Knöller 1, B Brings 1, T Pfeil 1, W König 1
PMCID: PMC1385019  PMID: 3181994

Abstract

IgE-binding factors (IgE-BF) have been shown to be important regulatory factors for IgE induction and suppression. The analysis of IgE-binding factor activity by a modified inhibition radioimmunoassay (RIA), as well as by monoclonal antibodies (mAb) was carried out in the supernatant of the Fc epsilon RII+ cell line RPMI-8866 as well as in human sera. Kinetics of IgE-BF showed optimal release from RPMI-8866 cells after 3-4 days. Gel filtration of the supernatant indicated binding activity at less than 100,000, 45,000 and 25,000 MW. Within normal human sera two peaks of IgE-BF activity were obtained at 45,000 and 25,000 MW. In sera with high IgE levels (atopic dermatitis) a peak at less than 100,000 was MW detected. Within this peak endogenous IgE was present. Addition of sodium dodecylsulphate induced a release of IgE-BF with a MW of 60,000.

Full text

PDF
58

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Capron M., Capron A., Dessaint J. P., Torpier G., Johansson S. G., Prin L. Fc receptors for IgE on human and rat eosinophils. J Immunol. 1981 Jun;126(6):2087–2092. [PubMed] [Google Scholar]
  2. Chen P. P., O'Hair C. H., Zuraw B. L., Katz D. H. A rapid screening assay for detection of IgE-binding factors in humans. J Immunol Methods. 1983 Mar 11;58(1-2):59–71. doi: 10.1016/0022-1759(83)90263-6. [DOI] [PubMed] [Google Scholar]
  3. Deguchi H., Suemura M., Ishizaka A., Ozaki Y., Kishimoto S., Yamamura Y., Kishimoto T. IgE class-specific suppressor T cells and factors in humans. J Immunol. 1983 Dec;131(6):2751–2756. [PubMed] [Google Scholar]
  4. Delespesse G., Sarfati M., Rubio-Trujillo M., Wolowiec T. IgE receptors on human lymphocytes. III. Expression of IgE receptors on mitogen-stimulated human mononuclear cells. Eur J Immunol. 1986 Sep;16(9):1043–1047. doi: 10.1002/eji.1830160903. [DOI] [PubMed] [Google Scholar]
  5. Geha R. S. Human IgE. J Allergy Clin Immunol. 1984 Aug;74(2):109–120. doi: 10.1016/0091-6749(84)90270-7. [DOI] [PubMed] [Google Scholar]
  6. Gonzalez-Molina A., Spiegelberg H. L. A subpopulation of normal human peripheral B lymphcytes that bind IgE. J Clin Invest. 1977 Apr;59(4):616–624. doi: 10.1172/JCI108679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gordon J., Webb A. J., Walker L., Guy G. R., Rowe M. Evidence for an association between CD23 and the receptor for a low molecular weight B cell growth factor. Eur J Immunol. 1986 Dec;16(12):1627–1630. doi: 10.1002/eji.1830161225. [DOI] [PubMed] [Google Scholar]
  8. Huff T. F., Ishizaka K. Formation of IgE-binding factors by human T-cell hybridomas. Proc Natl Acad Sci U S A. 1984 Mar;81(5):1514–1518. doi: 10.1073/pnas.81.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ikuta K., Takami M., Kim C. W., Honjo T., Miyoshi T., Tagaya Y., Kawabe T., Yodoi J. Human lymphocyte Fc receptor for IgE: sequence homology of its cloned cDNA with animal lectins. Proc Natl Acad Sci U S A. 1987 Feb;84(3):819–823. doi: 10.1073/pnas.84.3.819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ishizaka K. Immunoglobulin E (IgE). Methods Enzymol. 1985;116:76–94. doi: 10.1016/s0076-6879(85)16006-4. [DOI] [PubMed] [Google Scholar]
  11. Ishizaka K., Sandberg K. Formation of IgE binding factors by human T lymphocytes. J Immunol. 1981 May;126(5):1692–1696. [PubMed] [Google Scholar]
  12. Jensen J. R., Sand T. T., Spiegelberg H. L. Generation of IgE-binding and IgG-binding factors from human lymphoblastoid cell lines. Immunology. 1984 Sep;53(1):1–8. [PMC free article] [PubMed] [Google Scholar]
  13. Joseph M., Capron A., Ameisen J. C., Capron M., Vorng H., Pancré V., Kusnierz J. P., Auriault C. The receptor for IgE on blood platelets. Eur J Immunol. 1986 Mar;16(3):306–312. doi: 10.1002/eji.1830160318. [DOI] [PubMed] [Google Scholar]
  14. Kikutani H., Suemura M., Owaki H., Nakamura H., Sato R., Yamasaki K., Barsumian E. L., Hardy R. R., Kishimoto T. Fc epsilon receptor, a specific differentiation marker transiently expressed on mature B cells before isotype switching. J Exp Med. 1986 Nov 1;164(5):1455–1469. doi: 10.1084/jem.164.5.1455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. König W., Pfeiffer P., Rauschen I., Knöller J., Schönfeld W., Theobald K. The role of immunoglobulin E receptors in allergic diseases. Arzneimittelforschung. 1985;35(12A):1953–1957. [PubMed] [Google Scholar]
  16. Leung D. Y., Frankel R., Wood N., Geha R. S. Potentiation of human immunoglobulin E synthesis by plasma immunoglobulin E binding factors from patients with the hyperimmunoglobulin E syndrome. J Clin Invest. 1986 Mar;77(3):952–957. doi: 10.1172/JCI112395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nakajima T., Delespesse G. IgE receptors on human lymphocytes. I. Identification of the molecules binding to monoclonal anti-Fc epsilon receptor antibodies. Eur J Immunol. 1986 Jul;16(7):809–814. doi: 10.1002/eji.1830160715. [DOI] [PubMed] [Google Scholar]
  18. Prinz J. C., Endres N., Rank G., Ring J., Rieber E. P. Expression of Fc epsilon receptors on activated human T lymphocytes. Eur J Immunol. 1987 Jun;17(6):757–761. doi: 10.1002/eji.1830170604. [DOI] [PubMed] [Google Scholar]
  19. Rector E., Nakajima T., Rocha C., Duncan D., Lestourgeon D., Mitchell R. S., Fischer J., Sehon A. H., Delespesse G. Detection and characterization of monoclonal antibodies specific to IgE receptors on human lymphocytes by flow cytometry. Immunology. 1985 Jul;55(3):481–488. [PMC free article] [PubMed] [Google Scholar]
  20. Sarfati M., Nakajima T., Frost H., Kilccherr E., Delespesse G. Purification and partial biochemical characterization of IgE-binding factors secreted by a human B lymphoblastoid cell line. Immunology. 1987 Apr;60(4):539–545. [PMC free article] [PubMed] [Google Scholar]
  21. Sarfati M., Nutman T., Fonteyn C., Delespesse G. Presence of antigenic determinants common to Fc IgE receptors on human macrophages, T and B lymphocytes and IgE-binding factors. Immunology. 1986 Dec;59(4):569–575. [PMC free article] [PubMed] [Google Scholar]
  22. Sarfati M., Rector E., Rubio-Trujillo M., Wong K., Sehon A. H., Delespesse G. In vitro synthesis of IgE by human lymphocytes. III. IgE-potentiating activity of culture supernatants from Epstein-Barr virus (EBV) transformed B cells. Immunology. 1984 Oct;53(2):207–214. [PMC free article] [PubMed] [Google Scholar]
  23. Sarfati M., Rector E., Sehon A. H., Delespesse G. In vitro synthesis of IgE by human lymphocytes. IV. Suppression of the spontaneous IgE synthesis by IgE-binding factors secreted by tunicamycin-treated RPMI 8866 cells. Immunology. 1984 Dec;53(4):783–790. [PMC free article] [PubMed] [Google Scholar]
  24. Swendeman S., Thorley-Lawson D. A. The activation antigen BLAST-2, when shed, is an autocrine BCGF for normal and transformed B cells. EMBO J. 1987 Jun;6(6):1637–1642. doi: 10.1002/j.1460-2075.1987.tb02412.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Thompson L. F., Mellon M. H., Zeiger R. S., Spiegelberg H. L. Characterization with monoclonal antibodies of T lymphocytes bearing Fc receptors for IgE (T epsilon cells) and IgG (T gamma cells) in atopic patients. J Immunol. 1983 Dec;131(6):2772–2776. [PubMed] [Google Scholar]
  26. Thorley-Lawson D. A., Mann K. P. Early events in Epstein-Barr virus infection provide a model for B cell activation. J Exp Med. 1985 Jul 1;162(1):45–59. doi: 10.1084/jem.162.1.45. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Yodoi J., Hirashima M., Ishizaka K. Regulatory role of IgE-binding factors from rat T lymphocytes. V. The carbohydrate moieties in IgE-potentiating factors and IgE-suppressive factors. J Immunol. 1982 Jan;128(1):289–295. [PubMed] [Google Scholar]
  28. Young M. C., Leung D. Y., Geha R. S. Production of IgE-potentiating factor in man by T cell lines bearing Fc receptors for IgE. Eur J Immunol. 1984 Oct;14(10):871–878. doi: 10.1002/eji.1830141003. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES