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. 1989 Mar;66(3):445–450.

Presence of tumour necrosis factor or a related factor in human basophil/mast cells.

M Steffen 1, M Abboud 1, G K Potter 1, Y P Yung 1, M A Moore 1
PMCID: PMC1385235  PMID: 2703257

Abstract

The observation that mast cell products and cachectin/tumour necrosis factor (TNF) mediate similar responses suggested an investigation of cultured human basophil/mast cells for production of TNF. Using in situ hybridization and the avidin biotin-complex (ABC) immunoperoxidase method, we have demonstrated the presence of TNF mRNA in the cytoplasm and TNF protein in the granules of individual human basophil/mast cells. The production of TNF by these cells could explain many of their reported functions.

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Selected References

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