Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1989 May;67(1):16–20.

Activation of normal murine B cells by Echinococcus granulosus.

D A Cox 1, S Marshall-Clarke 1, J B Dixon 1
PMCID: PMC1385281  PMID: 2661414

Abstract

Echinococcus granulosus protoscolex (PSC) infection of BALB/c mice led, after 4 days, to raised numbers of cells forming plaques with trinitrophenyl-treated sheep red cells and bromelain-treated mouse red cells. The findings were similar in athymic and euthymic CBA mice. Activation of B cells was accompanied by secretion of immunoglobulin, as indicated by the reverse plaque technique. In addition, co-culture of PSC with the 7OZ/3 pre-B-cell led to the induction of differentiation, resulting in the expression of surface immunoglobulin (Ig). It is concluded that E. granulosus is a polyclonal activator of B cells inducing both transformation and differentiation, and that the effect is thymus-independent.

Full text

PDF
16

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ali-Khan Z. Humoral response to sheep red blood cells in C57L/J mice during early and chronic stages of infection with Echinococcus multilocularis cysts. Z Parasitenkd. 1979 Sep;59(3):259–265. doi: 10.1007/BF00927520. [DOI] [PubMed] [Google Scholar]
  2. Bailey M., Lloyd S., Martin S. C., Soulsby E. J. In vitro induction of lymphocyte responsiveness by a Strongylus vulgaris-derived mitogen. Z Parasitenkd. 1984;70(2):229–242. doi: 10.1007/BF00942226. [DOI] [PubMed] [Google Scholar]
  3. Corsini A. C., Clayton C., Askonas B. A., Ogilvie B. M. Suppressor cells and loss of B-cell potential in mice infected with Trypanosoma brucei. Clin Exp Immunol. 1977 Jul;29(1):122–131. [PMC free article] [PubMed] [Google Scholar]
  4. Cox D. A., Dixon J. B., Marshall-Clarke S. Transformation induced by Echinococcus granulosus protoscoleces in unprimed murine spleen cells: identity and MHC restriction of participating cell types. Immunology. 1986 Mar;57(3):461–466. [PMC free article] [PubMed] [Google Scholar]
  5. Crow M. K., Kunkel H. G. Activated B lymphocytes: stimulators of an augmented autologous mixed leukocyte reaction. Cell Immunol. 1985 Feb;90(2):555–568. doi: 10.1016/0008-8749(85)90220-5. [DOI] [PubMed] [Google Scholar]
  6. Cunningham A. J. A method of increased sensitivity for detecting single antibody-forming cells. Nature. 1965 Sep 4;207(5001):1106–1107. doi: 10.1038/2071106a0. [DOI] [PubMed] [Google Scholar]
  7. Cunningham A. J. Large numbers of cells in normal mice produce antibody components of isologous erythrocytes. Nature. 1974 Dec 20;252(5485):749–751. doi: 10.1038/252749a0. [DOI] [PubMed] [Google Scholar]
  8. Cunningham A. J., Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed] [Google Scholar]
  9. Dixon J. B., Jenkins P., Allan D. Immune recognition of Echinococcus granulosus. 1. Parasite-activated, primary transformation by normal murine lymph node cells. Parasite Immunol. 1982 Jan;4(1):33–45. doi: 10.1111/j.1365-3024.1982.tb00418.x. [DOI] [PubMed] [Google Scholar]
  10. Falanga P. B., D'Imperio Lima M. R., Coutinho A., Pereira da Silva L. Isotypic pattern of the polyclonal B cell response during primary infection by Plasmodium chabaudi and in immune-protected mice. Eur J Immunol. 1987 May;17(5):599–603. doi: 10.1002/eji.1830170504. [DOI] [PubMed] [Google Scholar]
  11. Hudson K. M., Byner C., Freeman J., Terry R. J. Immunodepression, high IgM levels and evasion of the immune response in murine trypanosomiasis. Nature. 1976 Nov 18;264(5583):256–258. doi: 10.1038/264256a0. [DOI] [PubMed] [Google Scholar]
  12. Judson D. G., Dixon J. B., Clarkson M. J., Pritchard J. Ovine hydatidosis: some immunological characteristics of the seronegative host. Parasitology. 1985 Oct;91(Pt 2):349–357. doi: 10.1017/s0031182000057413. [DOI] [PubMed] [Google Scholar]
  13. Marshall-Clarke S., Chayen A., Parkhouse R. M. Monoclonal antibody NIM-R2 shows differential reactivity with virgin and memory B cells. Eur J Immunol. 1982 Sep;12(9):733–738. doi: 10.1002/eji.1830120907. [DOI] [PubMed] [Google Scholar]
  14. Marshall-Clarke S., Cooke A., Hutchings P. R. Deficient production of anti-red cell autoantibodies by mice with an X-linked B lymphocyte defect. Eur J Immunol. 1979 Oct;9(10):820–823. doi: 10.1002/eji.1830091014. [DOI] [PubMed] [Google Scholar]
  15. Molinaro G. A., Dray S. Antibody coated erythrocytes as a manifold probe for antigens. Nature. 1974 Apr 5;248(448):515–517. doi: 10.1038/248515a0. [DOI] [PubMed] [Google Scholar]
  16. Ortiz-Ortiz L., Parks D. E., Rodriguez M., Weigle W. O. Polyclonal B lymphocyte activation during Trypanosoma cruzi infection. J Immunol. 1980 Jan;124(1):121–126. [PubMed] [Google Scholar]
  17. Paige C. J., Kincade P. W., Ralph P. Murine B cell leukemia line with inducible surface immunoglobulin expression. J Immunol. 1978 Aug;121(2):641–647. [PubMed] [Google Scholar]
  18. Pretell J. O., Falchuk Z. M. Generation of suppressor macrophages during the human autologous mixed lymphocyte reaction. Clin Exp Immunol. 1986 Jul;65(1):158–164. [PMC free article] [PubMed] [Google Scholar]
  19. Riley E. M., Dixon J. B. Experimental Echinococcus granulosus infection in mice: immunocytochemical analysis of lymphocyte populations in local lymphoid infections during early infection. Parasitology. 1987 Jun;94(Pt 3):523–532. doi: 10.1017/s0031182000055864. [DOI] [PubMed] [Google Scholar]
  20. Riley E. M., Dixon J. B., Jenkins P., Ross G. Echinococcus granulosus infection in mice: host responses during primary and secondary infection. Parasitology. 1986 Apr;92(Pt 2):391–403. doi: 10.1017/s0031182000064155. [DOI] [PubMed] [Google Scholar]
  21. Rittenberg M. B., Pratt K. L. Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen. Proc Soc Exp Biol Med. 1969 Nov;132(2):575–581. doi: 10.3181/00379727-132-34264. [DOI] [PubMed] [Google Scholar]
  22. Scher I., Frantz M. M., Steinberg A. D. The genetics of the immune response to a synthetic double-stranded RNA in a mutant CBA mouse strain. J Immunol. 1973 May;110(5):1396–1401. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES