Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1989 Aug;67(4):435–440.

Presentation of myelin basic protein by normal guinea-pig brain endothelial cells and its relevance to experimental allergic encephalomyelitis.

C E Wilcox 1, D G Healey 1, D Baker 1, D A Willoughby 1, J L Turk 1
PMCID: PMC1385310  PMID: 2475431

Abstract

Previous studies have shown that endothelial cells in the central nervous system (CNS) of normal guinea-pigs constitutively express certain MHC class II determinants, whilst the expression of other determinants is apparent during the acute phase of chronic relapsing experimental allergic encephalomyelitis (CREAE). The expression of MHC class II determinants is retained by endothelial cells derived from normal guinea-pig brain tissue and maintained in culture. This present study demonstrates that the MHC class II molecules on these cells can be recognized by allogeneic lymphocytes, resulting in a proliferative response which is enhanced by the addition of exogenous IL-2. The endothelial cells were incapable of presenting either purified protein derivative or ovalbumin, but they could present autologous myelin basic protein (MBP), an encephalitogen implicated in the pathogenesis of EAE. The resulting lymphocyte proliferative response was of the same magnitude as that obtained when a control population of macrophages was used to present MBP. These results, therefore, suggest that cerebrovascular endothelia have the potential to play a role in the pathogenesis of EAE.

Full text

PDF
435

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Antoniou A. V., el-Sady H., Butter C., Turk J. L. The modulation of class II histocompatibility antigens and 'activated' macrophage determinant in the spinal cord during the development of chronic relapsing experimental allergic encephalomyelitis (CREAE) in the guinea pig--relevance to the induction of remission? J Neuroimmunol. 1987 May;15(1):57–71. doi: 10.1016/0165-5728(87)90006-3. [DOI] [PubMed] [Google Scholar]
  2. Babbitt B. P., Allen P. M., Matsueda G., Haber E., Unanue E. R. Binding of immunogenic peptides to Ia histocompatibility molecules. 1985 Sep 26-Oct 2Nature. 317(6035):359–361. doi: 10.1038/317359a0. [DOI] [PubMed] [Google Scholar]
  3. Burger R., Clement L., Schroer J., Chiba J., Shevach E. M. Monoclonal antibodies to guinea pig Ia antigens. I. Production, serologic, and immunochemical characterization. J Immunol. 1981 Jan;126(1):32–37. [PubMed] [Google Scholar]
  4. Butter C., Healey D. G., Agha N., Turk J. L. An immunoelectron microscopical study of the expression of class II MHC and a T lymphocyte surface marker during chronic relapsing experimental allergic encephalomyelitis. J Neuroimmunol. 1988 Nov;20(1):45–51. doi: 10.1016/0165-5728(88)90113-0. [DOI] [PubMed] [Google Scholar]
  5. Chain B. M., Kay P. M., Feldmann M. The cellular pathway of antigen presentation: biochemical and functional analysis of antigen processing in dendritic cells and macrophages. Immunology. 1986 Jun;58(2):271–276. [PMC free article] [PubMed] [Google Scholar]
  6. Chesnut R. W., Colon S. M., Grey H. M. Antigen presentation by normal B cells, B cell tumors, and macrophages: functional and biochemical comparison. J Immunol. 1982 Apr;128(4):1764–1768. [PubMed] [Google Scholar]
  7. Gaspari A. A., Katz S. I. Induction and functional characterization of class II MHC (Ia) antigens on murine keratinocytes. J Immunol. 1988 May 1;140(9):2956–2963. [PubMed] [Google Scholar]
  8. Gautam A. M., Glynn P. Lewis rat lymphoid dendritic cells can efficiently present homologous myelin basic protein to encephalitogenic lymphocytes. J Neuroimmunol. 1989 Apr;22(2):113–121. doi: 10.1016/0165-5728(89)90041-6. [DOI] [PubMed] [Google Scholar]
  9. Healey D. G., Agha N., Turk J. L. Behaviour of guinea pig T cells stimulated by antigen, allo-antigen and mitogen. Int Arch Allergy Appl Immunol. 1988;87(2):134–142. doi: 10.1159/000234663. [DOI] [PubMed] [Google Scholar]
  10. McCarron R. M., Spatz M., Kempski O., Hogan R. N., Muehl L., McFarlin D. E. Interaction between myelin basic protein-sensitized T lymphocytes and murine cerebral vascular endothelial cells. J Immunol. 1986 Dec 1;137(11):3428–3435. [PubMed] [Google Scholar]
  11. Pryce G., Male D., Sedgwick J. Antigen presentation in brain: brain endothelial cells are poor stimulators of T-cell proliferation. Immunology. 1989 Feb;66(2):207–212. [PMC free article] [PubMed] [Google Scholar]
  12. Rosenthal A. S., Shevach E. M. Function of macrophages in antigen recognition by guinea pig T lymphocytes. I. Requirement for histocompatible macrophages and lymphocytes. J Exp Med. 1973 Nov 1;138(5):1194–1212. doi: 10.1084/jem.138.5.1194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schwartz B. D., Paul W. E., Shevach E. M. Guinea-pig Ia antigens: functional significance and chemical characterization. Transplant Rev. 1976;30:174–196. doi: 10.1111/j.1600-065x.1976.tb00220.x. [DOI] [PubMed] [Google Scholar]
  14. Shimonkevitz R., Kappler J., Marrack P., Grey H. Antigen recognition by H-2-restricted T cells. I. Cell-free antigen processing. J Exp Med. 1983 Aug 1;158(2):303–316. doi: 10.1084/jem.158.2.303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sobel R. A., Blanchette B. W., Bhan A. K., Colvin R. B. The immunopathology of experimental allergic encephalomyelitis. II. Endothelial cell Ia increases prior to inflammatory cell infiltration. J Immunol. 1984 May;132(5):2402–2407. [PubMed] [Google Scholar]
  16. Stingl G., Katz S. I., Clement L., Green I., Shevach E. M. Immunologic functions of Ia-bearing epidermal Langerhans cells. J Immunol. 1978 Nov;121(5):2005–2013. [PubMed] [Google Scholar]
  17. Streicher H. Z., Berkower I. J., Busch M., Gurd F. R., Berzofsky J. A. Antigen conformation determines processing requirements for T-cell activation. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6831–6835. doi: 10.1073/pnas.81.21.6831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Traugott U., Raine C. S., McFarlin D. E. Acute experimental allergic encephalomyelitis in the mouse: immunopathology of the developing lesion. Cell Immunol. 1985 Mar;91(1):240–254. doi: 10.1016/0008-8749(85)90047-4. [DOI] [PubMed] [Google Scholar]
  19. Traugott U., Scheinberg L. C., Raine C. S. On the presence of Ia-positive endothelial cells and astrocytes in multiple sclerosis lesions and its relevance to antigen presentation. J Neuroimmunol. 1985 Apr;8(1):1–14. doi: 10.1016/s0165-5728(85)80043-6. [DOI] [PubMed] [Google Scholar]
  20. Wilcox C. E., Baker D., Butter C., Willoughby D. A., Turk J. L. Differential expression of guinea pig class II major histocompatibility complex antigens on vascular endothelial cells in vitro and in experimental allergic encephalomyelitis. Cell Immunol. 1989 Apr 15;120(1):82–91. doi: 10.1016/0008-8749(89)90176-7. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES