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. 1989 Aug;67(4):447–452.

Allergic arthritis induced by cationic proteins: role of molecular weight.

P L van Lent 1, C Dekker 1, J Mosterd 1, L van den Bersselaar 1, W B van den Berg 1
PMCID: PMC1385312  PMID: 2767709

Abstract

Previous studies have shown that chronic murine allergic arthritis can only be induced with cationized BSA, related to excellent retention of the cationic antigen in the joint. We now investigate the impact of size of cationic proteins on their potential to induce this form of arthritis. After intra-articular injection, antigen retention is much enhanced with high molecular weight cationized proteins, like albumin or immunoglobulin, compared to small-sized proteins like myoglobulin and lysozyme. Consequently, severe chronic arthritis was only found with the former ones. The role of size is further substantiated with poly-L-lysine-coupled lysozyme. This derivative shows excellent retention in vivo and causes a chronic destructive arthritis in preimmunized mice, in contrast to the poor arthritis seen with native cationic lysozyme. Control experiments made it clear that antigen retention is the most important denominator and that differences in chronicity are not related to gross variations in T-cell reactivity. Retention studies in vitro revealed that the potential to bind to joint structures is similar for the various proteins, suggesting that in vivo conditions determine size-related differences in antigen clearance. Our data indicate that cationicity per se does not make a protein a proper arthritogen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Apple R. J., Domen P. L., Muckerheide A., Michael J. G. Cationization of protein antigens. IV. Increased antigen uptake by antigen-presenting cells. J Immunol. 1988 May 15;140(10):3290–3295. [PubMed] [Google Scholar]
  2. Aston W. P., Ward T. L., Cooke T. D. The specific in vitro antibody-mediated retention of bovine serum albumin by porcine hyaline articular cartilage. Clin Exp Immunol. 1983 May;52(2):280–286. [PMC free article] [PubMed] [Google Scholar]
  3. Batsford S. R., Takamiya M., Vogt A. A model of in situ immune complex glomerulonephritis in the rat employing cationized ferritin. Clin Nephrol. 1980 Nov;14(5):211–216. [PubMed] [Google Scholar]
  4. Brackertz D., Mitchell G. F., Mackay I. R. Antigen-induced arthritis in mice. I. Induction of arthritis in various strains of mice. Arthritis Rheum. 1977 Apr;20(3):841–850. doi: 10.1002/art.1780200314. [DOI] [PubMed] [Google Scholar]
  5. Cooke T. D., Hurd E. R., Ziff M., Jasin H. E. The pathogenesis of chronic inflammation in experimental antigen-induced arthritis. II. Preferential localization of antigen-antibody complexes to collagenous tissues. J Exp Med. 1972 Feb 1;135(2):323–338. doi: 10.1084/jem.135.2.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cooke T. D., Maeda M. Polymorphonuclear cell interactions with immune complexes in cartilage. Arthritis Rheum. 1979 Dec;22(12):1416–1416. doi: 10.1002/art.1780221221. [DOI] [PubMed] [Google Scholar]
  7. Danon D., Goldstein L., Marikovsky Y., Skutelsky E. Use of cationized ferritin as a label of negative charges on cell surfaces. J Ultrastruct Res. 1972 Mar;38(5):500–510. doi: 10.1016/0022-5320(72)90087-1. [DOI] [PubMed] [Google Scholar]
  8. Greenwald R. A. Connective tissue lysozyme in health and disease. Semin Arthritis Rheum. 1976 Aug;6(1):35–51. doi: 10.1016/s0049-0172(76)80004-2. [DOI] [PubMed] [Google Scholar]
  9. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  10. Habeeb A. F. Determination of free amino groups in proteins by trinitrobenzenesulfonic acid. Anal Biochem. 1966 Mar;14(3):328–336. doi: 10.1016/0003-2697(66)90275-2. [DOI] [PubMed] [Google Scholar]
  11. Hollister J. R., Mannik M. Antigen retention in joint tissues in antigen-induced synovitis. Clin Exp Immunol. 1974 Apr;16(4):615–627. [PMC free article] [PubMed] [Google Scholar]
  12. Jasin H. E. Mechanism of trapping of immune complexes in joint collagenous tissues. Clin Exp Immunol. 1975 Dec;22(3):473–485. [PMC free article] [PubMed] [Google Scholar]
  13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  14. Lens J. W., van den Berg W. B., van de Putte L. B., Zwarts W. A. Flare of antigen-induced arthritis in mice after intravenous challenge. Kinetics of antigen in the circulation and localization of antigen in the arthritic and noninflamed joint. Arthritis Rheum. 1986 May;29(5):665–674. doi: 10.1002/art.1780290512. [DOI] [PubMed] [Google Scholar]
  16. Shen W. C., Yang D., Ryser H. J. Colorimetric determination of microgram quantities of polylysine by trypan blue precipitation. Anal Biochem. 1984 Nov 1;142(2):521–524. doi: 10.1016/0003-2697(84)90500-1. [DOI] [PubMed] [Google Scholar]
  17. Teuscher C., Donaldson D. M. The deposition and formation of immune complexes in collagenous tissues. Clin Immunol Immunopathol. 1979 May;13(1):56–66. doi: 10.1016/0090-1229(79)90020-5. [DOI] [PubMed] [Google Scholar]
  18. Vogt A., Batsford S., Rodríguez-Iturbe B., García R. Cationic antigens in poststreptococcal glomerulonephritis. Clin Nephrol. 1983 Dec;20(6):271–279. [PubMed] [Google Scholar]
  19. Wallis W. J., Simkin P. A., Nelp W. B., Foster D. M. Intraarticular volume and clearance in human synovial effusions. Arthritis Rheum. 1985 Apr;28(4):441–449. doi: 10.1002/art.1780280413. [DOI] [PubMed] [Google Scholar]
  20. van Lent P. L., van den Berg W. B., Schalkwijk J., van de Putte L. B., van den Bersselaar L. Allergic arthritis induced by cationic antigens: relationship of chronicity with antigen retention and T-cell reactivity. Immunology. 1987 Oct;62(2):265–272. [PMC free article] [PubMed] [Google Scholar]
  21. van den Berg W. B., Kruijsen M. W., van de Putte L. B., van Beusekom H. J., van der Sluis-van der Pol M., Zwarts W. A. Antigen-induced and zymosan-induced arthritis in mice: studies on in vivo cartilage proteoglycan synthesis and chondrocyte death. Br J Exp Pathol. 1981 Jun;62(3):308–316. [PMC free article] [PubMed] [Google Scholar]
  22. van den Berg W. B., van Lent P. L., van de Putte L. B., Zwarts W. A. Electrical charge of hyaline articular cartilage: its role in the retention of anionic and cationic proteins. Clin Immunol Immunopathol. 1986 May;39(2):187–197. doi: 10.1016/0090-1229(86)90083-8. [DOI] [PubMed] [Google Scholar]
  23. van den Berg W. B., van de Putte L. B. Electrical charge of the antigen determines its localization in the mouse knee joint. Deep penetration of cationic BSA in hyaline articular cartilage. Am J Pathol. 1985 Nov;121(2):224–234. [PMC free article] [PubMed] [Google Scholar]
  24. van den Berg W. B., van de Putte L. B., Zwarts W. A., Joosten L. A. Electrical charge of the antigen determines intraarticular antigen handling and chronicity of arthritis in mice. J Clin Invest. 1984 Nov;74(5):1850–1859. doi: 10.1172/JCI111604. [DOI] [PMC free article] [PubMed] [Google Scholar]

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