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. 1989 Oct;68(2):185–189.

Antigen-specific inhibition of IL-2 and IL-3 production in contact sensitivity to TNP.

J Marcinkiewicz 1, B Chain 1
PMCID: PMC1385414  PMID: 2530159

Abstract

The production of IL-2 and IL-3 by T cells from mice which had been contact sensitized to TNP and/or tolerized by intravenous injections of TNBS was assayed. Contact sensitization rapidly primes T cells, so that they respond to in vitro restimulation with haptenated syngeneic cells by producing IL-2 and IL-3. This production is strongly inhibited, in an antigen-specific manner, in tolerized mice. At least part of this inhibition can be attributed to the action of suppressor T cells that act by preventing the activation of lymphokine production in vitro. Lymphokine production thus closely parallels the in vivo delayed-type hypersensitivity (DTH) reaction in this system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asherson G. L., Colizzi V., Zembala M. An overview of T-suppressor cell circuits. Annu Rev Immunol. 1986;4:37–68. doi: 10.1146/annurev.iy.04.040186.000345. [DOI] [PubMed] [Google Scholar]
  2. Cillari E., Liew F. Y., Lelchuk R. Suppression of interleukin-2 production by macrophages in genetically susceptible mice infected with Leishmania major. Infect Immun. 1986 Nov;54(2):386–394. doi: 10.1128/iai.54.2.386-394.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Davignon J. L., Kimoto M., Kindler V., De Kossodo S., Vassalli P., Izui S. Selective production of interleukin 3 (IL3) and granulocyte-macrophage colony-stimulating factor (GM-CSF) in vitro by murine L3T4+ T cells: lack of spontaneous IL3 and GM-CSF production by Ly-2-/L3T4- lpr subset. Eur J Immunol. 1988 Sep;18(9):1367–1372. doi: 10.1002/eji.1830180910. [DOI] [PubMed] [Google Scholar]
  4. Dwyer J. M., Kantor F. S. Regulation of delayed hypersensitivity. Failure to transfer delayed hypersensitivity to desensitized guinea pigs. J Exp Med. 1973 Jan 1;137(1):32–41. doi: 10.1084/jem.137.1.32. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jenkins M. K., Schwartz R. H. Antigen presentation by chemically modified splenocytes induces antigen-specific T cell unresponsiveness in vitro and in vivo. J Exp Med. 1987 Feb 1;165(2):302–319. doi: 10.1084/jem.165.2.302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kimber I., Pierce B. B., Mitchell J. A., Kinnaird A. Depression of lymph node cell proliferation induced by oxazolone. Int Arch Allergy Appl Immunol. 1987;84(3):256–262. doi: 10.1159/000234432. [DOI] [PubMed] [Google Scholar]
  7. Lamb J. R., Skidmore B. J., Green N., Chiller J. M., Feldmann M. Induction of tolerance in influenza virus-immune T lymphocyte clones with synthetic peptides of influenza hemagglutinin. J Exp Med. 1983 May 1;157(5):1434–1447. doi: 10.1084/jem.157.5.1434. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lefkovits L., Aarden L. A., Corley R. B. Ratio-dominance model of suppression: an analysis by limiting dilution. Immunology. 1980 Oct;41(2):407–413. [PMC free article] [PubMed] [Google Scholar]
  9. Macatonia S. E., Edwards A. J., Knight S. C. Dendritic cells and the initiation of contact sensitivity to fluorescein isothiocyanate. Immunology. 1986 Dec;59(4):509–514. [PMC free article] [PubMed] [Google Scholar]
  10. Marcinkiewicz J., Bereta M., Malinowski J., Ptak W. The induction of oxazolone-specific T suppressor afferent cells in mice by hapten-modified isologous IgG. Eur J Immunol. 1984 Aug;14(8):759–762. doi: 10.1002/eji.1830140818. [DOI] [PubMed] [Google Scholar]
  11. Mosmann T. R., Cherwinski H., Bond M. W., Giedlin M. A., Coffman R. L. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol. 1986 Apr 1;136(7):2348–2357. [PubMed] [Google Scholar]
  12. Schmitt J., Wagner H., Heeg K. Reactivity of Ly-2+ T cells against 2,4,6-trinitrophenyl (TNP)-modified syngeneic stimulator cells: specificity, frequency of interleukin 2-producing Ly-2+ helper T cells and clonal segregation from Ly-2+ cytotoxic T lymphocytes. Eur J Immunol. 1988 Mar;18(3):325–332. doi: 10.1002/eji.1830180302. [DOI] [PubMed] [Google Scholar]
  13. Tada H., Shiho O., Kuroshima K., Koyama M., Tsukamoto K. An improved colorimetric assay for interleukin 2. J Immunol Methods. 1986 Nov 6;93(2):157–165. doi: 10.1016/0022-1759(86)90183-3. [DOI] [PubMed] [Google Scholar]
  14. Van Loveren H., Askenase P. W. Delayed-type hypersensitivity is mediated by a sequence of two different T cell activities. J Immunol. 1984 Nov;133(5):2397–2401. [PubMed] [Google Scholar]
  15. Wagner H., Heeg K. Two distinct signals regulate induction of IL-2 responsiveness in CD8+ murine T cells. Immunology. 1988 Jul;64(3):433–438. [PMC free article] [PubMed] [Google Scholar]
  16. Zöller M., Andrighetto G. Induction of tolerance towards TNP entails down-regulation of an autoimmune attack. Immunology. 1988 Feb;63(2):205–212. [PMC free article] [PubMed] [Google Scholar]

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