Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1989 Oct;68(2):233–239.

Some properties of dendritic macrophages from peripheral lymph.

R Barfoot 1, S Denham 1, L A Gyure 1, J G Hall 1, S M Hobbs 1, L E Jackson 1, D Robertson 1
PMCID: PMC1385423  PMID: 2807381

Abstract

Peripheral lymph was collected from the skin and liver of sheep, and from the intestine of rats. The dendritic macrophages contained in it were isolated by centrifuging the lymph over a layer of 'Nycodenz'. Similar cells were produced by culturing mononuclear cells from venous blood, but the yields were very small. The numbers of dendritic cells in the lymph from the legs of sheep increased five-fold after xylene had been applied to the skin. Dendritic macrophages displayed abundant class II histocompatibility antigens on their surfaces, as well as immunoglobulins. Although the latter were probably acquired passively, they remained present for several days on cells cultured in vitro. When in vitro, dendritic cells could be shown to phagocytose marker particles, such as latex beads, but their performance was unimpressive compared to macrophages from the peritoneal cavities of rats. In contrast, their ability to phagocytose rapidly T4 phage or influenza viruses unequivocal and striking.

Full text

PDF
236

Images in this article

235Figure 2
on p.235
236Figure 3
on p.236
236Figure 4
on p.236
236Figure 5
on p.236
236Figure 6
on p.236
237Figure 7
on p.237
237Figure 8
on p.237

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Austyn J. M. Lymphoid dendritic cells. Immunology. 1987 Oct;62(2):161–170. [PMC free article] [PubMed] [Google Scholar]
  2. Chain B. M., Kay P. M., Feldmann M. The cellular pathway of antigen presentation: biochemical and functional analysis of antigen processing in dendritic cells and macrophages. Immunology. 1986 Jun;58(2):271–276. [PMC free article] [PubMed] [Google Scholar]
  3. Crow M. K., Kunkel H. G. Human dendritic cells: major stimulators of the autologous and allogeneic mixed leucocyte reactions. Clin Exp Immunol. 1982 Aug;49(2):338–346. [PMC free article] [PubMed] [Google Scholar]
  4. De Martini J. C., Fiscus S. A., Pearson L. D. Macrophages in efferent lymph of sheep and their role in lectin-induced lymphocyte blastogenesis. Int Arch Allergy Appl Immunol. 1983;72(2):110–115. doi: 10.1159/000234851. [DOI] [PubMed] [Google Scholar]
  5. Emery D. L., MacHugh N. D., Ellis J. A. The properties and functional activity of non-lymphoid cells from bovine afferent (peripheral) lymph. Immunology. 1987 Oct;62(2):177–183. [PMC free article] [PubMed] [Google Scholar]
  6. HALL J. G., MORRIS B. The output of cells in lymph from the popliteal node of sheep. Q J Exp Physiol Cogn Med Sci. 1962 Oct;47:360–369. doi: 10.1113/expphysiol.1962.sp001620. [DOI] [PubMed] [Google Scholar]
  7. Hall J. G., Birbeck M. S., Robertson D., Peppard J., Orlans E. The use of detergents and immunoperoxidase reagents for the ultrastructural demonstration of internal immunoglobulin in lymph cells. J Immunol Methods. 1978;19(4):351–359. doi: 10.1016/0022-1759(78)90019-4. [DOI] [PubMed] [Google Scholar]
  8. Hall J. G. Effect of skin painting with oxazolone on the local extravasation of mononuclear cells in sheep. Ciba Found Symp. 1980;71:197–209. doi: 10.1002/9780470720547.ch11. [DOI] [PubMed] [Google Scholar]
  9. Hall J. G., Hopkins J., Orlans E. Studies on the lymphocytes of sheep. III. Destination of lymph-borne immunoblasts in relation to their tissue of origin. Eur J Immunol. 1977 Jan;7(1):30–37. doi: 10.1002/eji.1830070108. [DOI] [PubMed] [Google Scholar]
  10. Hall J. G., Robertson D. Phagocytosis, in vivo, of immune complexes by dendritic cells in the lymph of sheep. Int Arch Allergy Appl Immunol. 1984;73(2):155–161. doi: 10.1159/000233457. [DOI] [PubMed] [Google Scholar]
  11. Hall J. G. Studies of the cells in the afferent and efferent lymph of lymph nodes draining the site of skin homografts. J Exp Med. 1967 May 1;125(5):737–754. doi: 10.1084/jem.125.5.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hall J. G. Studies on the adjuvant action of beryllium. I. Effects on individual lymph nodes. Immunology. 1984 Sep;53(1):105–113. [PMC free article] [PubMed] [Google Scholar]
  13. Hall J. Lymphocyte recirculation and the gut: the cellular basis of humoral immunity in the intestine. Blood Cells. 1979 Aug;5(3):479–497. [PubMed] [Google Scholar]
  14. Knight S. C., Farrant J., Bryant A., Edwards A. J., Burman S., Lever A., Clarke J., Webster A. D. Non-adherent, low-density cells from human peripheral blood contain dendritic cells and monocytes, both with veiled morphology. Immunology. 1986 Apr;57(4):595–603. [PMC free article] [PubMed] [Google Scholar]
  15. LASCELLES A. K., MORRIS B. Surgical techniques for the collection of lymph from unanaesthetized sheep. Q J Exp Physiol Cogn Med Sci. 1961 Jul;46:199–205. doi: 10.1113/expphysiol.1961.sp001536. [DOI] [PubMed] [Google Scholar]
  16. McFarlin D. E., Balfour B. Contact sensitivity in the pig. Immunology. 1973 Dec;25(6):995–1009. [PMC free article] [PubMed] [Google Scholar]
  17. Morris B., Salami M. A. The blood and lymphatic capillaries of lymph nodes in the sheep foetus and their involvement in cell traffic. Lymphology. 1987 Dec;20(4):244–251. [PubMed] [Google Scholar]
  18. Pedersen N. C., Morris B. The role of the lymphatic system in the rejection of homografts: a study of lymph from renal transplants. J Exp Med. 1970 May 1;131(5):936–969. doi: 10.1084/jem.131.5.936. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pugh C. W., MacPherson G. G., Steer H. W. Characterization of nonlymphoid cells derived from rat peripheral lymph. J Exp Med. 1983 Jun 1;157(6):1758–1779. doi: 10.1084/jem.157.6.1758. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Scollay R., Hall J., Orlans E. Studies on the lymphocytes of sheep. II. Some properties of cells in various compartments of the recirculating lymphocyte pool. Eur J Immunol. 1976 Feb;6(2):121–125. doi: 10.1002/eji.1830060210. [DOI] [PubMed] [Google Scholar]
  21. Smith J. B., McIntosh G. H., Morris B. The traffic of cells through tissues: a study of peripheral lymph in sheep. J Anat. 1970 Jul;107(Pt 1):87–100. [PMC free article] [PubMed] [Google Scholar]
  22. Spry C. J., Pflug A. J., Janossy G., Humphrey J. H. Large mononuclear (veiled) cells like 'Ia-like' membrane antigens in human afferent lympn. Clin Exp Immunol. 1980 Mar;39(3):750–755. [PMC free article] [PubMed] [Google Scholar]
  23. Steinman R. M., Nussenzweig M. C. Dendritic cells: features and functions. Immunol Rev. 1980;53:127–147. doi: 10.1111/j.1600-065x.1980.tb01042.x. [DOI] [PubMed] [Google Scholar]
  24. Styles J. M., Dean C. J., Gyure L. A., Hobbs S. M., Hall J. G. The production of hybridomas from the gut associated lymphoid tissue of tumour bearing rats. II. Peripheral intestinal lymph as a source of IgA producing cells. Clin Exp Immunol. 1984 Aug;57(2):365–370. [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES