Skip to main content
PMC home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1970 Sep;210(2):421–432.3. doi: 10.1113/jphysiol.1970.sp009218

Properties of kallikrein-containing granules isolated from the submaxillary gland of the guinea-pig

K D Bhoola, P F Heap
PMCID: PMC1395569  PMID: 5501268

Abstract

1. Granular fractions of high purity consisting of subcellular kallikrein- and amylase-storing organelles have been isolated from homogenates of guinea-pig submaxillary gland.

2. The isolated kallikrein- and amylase-containing granules closely resembled secretory granules observed in situ in serous acinar cells in intra-granular appearance, size and histochemical reaction.

3. The subcellular, histochemical and ultrastructural studies indicate that the serine protease, kallikrein, is like amylase an exocrine enzyme with a functional role in saliva.

Full text

PDF
421

Images in this article

432-1A
on p.432-1
432-1B
on p.432-1
432-2A
on p.432-2
432-2B
on p.432-2
432-3Plate 3
on p.432-3

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bhoola K. D., Heap P. F. Electron microscopy and histochemistry of isolated kallikrein granules. Br J Pharmacol. 1970 Feb;38(2):435P–436P. [PMC free article] [PubMed] [Google Scholar]
  2. Bhoola K. D. Intracellular distribution of submaxillary kallikrein. J Physiol. 1968 May;196(2):431–445. doi: 10.1113/jphysiol.1968.sp008516. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bhoola K. D., Ogle C. W. The subcellular localization of kallikrein, amylase and acetylcholine in the submaxillary gland of the guinea-pig. J Physiol. 1966 Jun;184(3):663–672. doi: 10.1113/jphysiol.1966.sp007939. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ekfors T. O., Hopsu-Havu V. K., Malniharju T. Increases vascular permeability caused by the trypsin-like enzymes purified from rat submandibular gland. Acta Physiol Scand. 1969 Jan-Feb;75(1):157–160. doi: 10.1111/j.1748-1716.1969.tb04367.x. [DOI] [PubMed] [Google Scholar]
  5. Feldberg W., Guimarāis J. A. Some observations on salivary secretion. J Physiol. 1935 Aug 22;85(1):15–36. doi: 10.1113/jphysiol.1935.sp003299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Heap P. F., Bhoola K. D. Histochemical localization of kallikrein granules in the submaxillary gland of the guinea-pig. J Anat. 1969 Nov;105(Pt 3):525–532. [PMC free article] [PubMed] [Google Scholar]
  7. Maunsbach A. B. Isolation and purification of acid phosphatase-containing autofluorescent granules from homogenates of rat kidney cortex. J Ultrastruct Res. 1966 Sep;16(1):13–34. doi: 10.1016/s0022-5320(66)80020-5. [DOI] [PubMed] [Google Scholar]
  8. RICHARDSON K. C., JARETT L., FINKE E. H. Embedding in epoxy resins for ultrathin sectioning in electron microscopy. Stain Technol. 1960 Nov;35:313–323. doi: 10.3109/10520296009114754. [DOI] [PubMed] [Google Scholar]
  9. Riekkinen P. J., Ekfors T. O., Hopsu V. K. Purification and characteristics of an alkaline protease from rat-submandibular gland. Biochim Biophys Acta. 1966 Jun 15;118(3):604–620. doi: 10.1016/s0926-6593(66)80101-7. [DOI] [PubMed] [Google Scholar]
  10. SLATER T. F., PLANTEROSE D. N. An assay procedure for a succinate-neotetrazolium-reductase system. Biochem J. 1960 Mar;74:591–596. doi: 10.1042/bj0740591. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schachter M. Kallikreins and kinins. Physiol Rev. 1969 Jul;49(3):509–547. doi: 10.1152/physrev.1969.49.3.509. [DOI] [PubMed] [Google Scholar]
  12. Searcy R. L., Hayashi S., Hardy E. M., Berk J. E. The interaction of human serum protein fractions with the starch-iodine complex. Clin Chim Acta. 1965 Dec;12(6):631–638. doi: 10.1016/0009-8981(65)90145-2. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES