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. 1970 Aug;209(2):487–511. doi: 10.1113/jphysiol.1970.sp009176

Observations on the change in shape of blood platelets brought about by adenosine diphosphate

G V R Born
PMCID: PMC1395740  PMID: 5499539

Abstract

1. An optical method used for measuring platelet aggregation was adapted for measurements of the change in shape of platelets that rapidly follows the addition of the aggregating agent adenosine diphosphate (ADP).

2. Measurements were made using dilute suspension of platelets with sufficient EDTA to prevent their aggregation.

3. Measurements of the velocity and magnitude of the optical effects of the shape change were highly reproducible.

4. Volumetric measurements showed that the shape change is not associated with an increase in mean platelet volume.

5. The velocity of the shape change had a temperature coefficient of about 4·5.

6. The velocity and magnitude of the shape change were not affected by pH between 5·8 and 9·2.

7. The dependence of the velocity of the shape change on the ADP concentration was in accordance with Michaelis—Menton kinetics. The Km was about 7·2 × 10-7M.

8. The velocity of the shape change was inhibited competitively by ATP, adenosine and 2-chloroadenosine but not by AMP. When the inhibitors were added after the maximum of the shape change they caused a concentration-dependent diminution in the record of the change.

9. The results suggest that the shape change is initiated by reaction of the agonist ADP with specific receptor sites on the platelet membrane which leads to energy-requiring changes in the structures responsible for maintaining the disk shape of normal platelets.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BORN G. V. Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature. 1962 Jun 9;194:927–929. doi: 10.1038/194927b0. [DOI] [PubMed] [Google Scholar]
  2. BORN G. V., BRICKNELL J. The uptake of 5-hydroxytryptamine by blood platelets in the cold. J Physiol. 1959 Jun 23;147(1):153–161. doi: 10.1113/jphysiol.1959.sp006230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BORN G. V., CROSS M. J. THE AGGREGATION OF BLOOD PLATELETS. J Physiol. 1963 Aug;168:178–195. doi: 10.1113/jphysiol.1963.sp007185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BORN G. V., GILLSON R. E. Studies on the uptake of 5-hydroxytryptamine by blood platelets. J Physiol. 1959 Jun 11;146(3):472–491. doi: 10.1113/jphysiol.1959.sp006206. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. BORN G. V., HASLAM R. J., GOLDMAN M. COMPARATIVE EFFECTIVENESS OF ADENOSINE ANALOGUES AS INHIBITORS OF BLOOD-PLATELET AGGREGATION AND AS VASODILATORS IN MAN. Nature. 1965 Feb 13;205:678–680. doi: 10.1038/205678a0. [DOI] [PubMed] [Google Scholar]
  6. BORN G. V. STRONG INHIBITION BY 2-CHLOROADENOSINE OF THE AGGREGATION OF BLOOD PLATELETS BY ADENOSINE DIPHOSPHATE. Nature. 1964 Apr 4;202:95–96. doi: 10.1038/202095b0. [DOI] [PubMed] [Google Scholar]
  7. Behnke O. Further studies on microtubules. A marginal bundle in human and rat thrombocytes. J Ultrastruct Res. 1965 Dec;13(5):469–477. doi: 10.1016/s0022-5320(65)90009-2. [DOI] [PubMed] [Google Scholar]
  8. Born G. V., Day M., Stockbridge A. The uptake of amines by human erythrocytes in vitro. J Physiol. 1967 Nov;193(2):405–418. doi: 10.1113/jphysiol.1967.sp008366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Born G. V., Hume M. Effects of the numbers and sizes of platelet aggregates on the optical density of plasma. Nature. 1967 Sep 2;215(5105):1027–1029. doi: 10.1038/2151027a0. [DOI] [PubMed] [Google Scholar]
  10. Born G. V., Mills D. C. Potentiation of the inhibitory effect of adenosine on platelet aggregation by drugs that prevent its uptake. J Physiol. 1969 May;202(1):41P–42P. [PubMed] [Google Scholar]
  11. Born G. V. Quantitative investigations of the rapid swelling reaction of blood platelets. J Physiol. 1969 Jun;202(2):93P–94P. [PubMed] [Google Scholar]
  12. Bull B. S., Zucker M. B. Changes in platelet volume produced by temperature, metabolic inhibitors, and aggregating agents. Proc Soc Exp Biol Med. 1965 Nov;120(2):296–301. doi: 10.3181/00379727-120-30516. [DOI] [PubMed] [Google Scholar]
  13. DAVIES R. E. A MOLECULAR THEORY OF MUSCLE CONTRACTION: CALCIUM-DEPENDENT CONTRACTIONS WITH HYDROGEN BOND FORMATION PLUS ATP-DEPENDENT EXTENSIONS OF PART OF THE MYOSIN-ACTIN CROSS-BRIDGES. Nature. 1963 Sep 14;199:1068–1074. doi: 10.1038/1991068a0. [DOI] [PubMed] [Google Scholar]
  14. GORDON G., PHILLIPS C. G. Slow and rapid components in a flexor muscle. Q J Exp Physiol Cogn Med Sci. 1953;38(1):35–45. doi: 10.1113/expphysiol.1953.sp001005. [DOI] [PubMed] [Google Scholar]
  15. Jahn W. Kreislaufwirkungen 5'-substituierter Adenosinderivate. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1965 Jun 1;251(1):95–104. [PubMed] [Google Scholar]
  16. MACMILLAN D. C., OLIVER M. F. THE INITIAL CHANGES IN PLATELET MORPHOLOGY FOLLOWING THE ADDITION OF ADENOSINE DIPHOSPHATE. J Atheroscler Res. 1965 Jul-Aug;5(4):440–444. doi: 10.1016/s0368-1319(65)80081-3. [DOI] [PubMed] [Google Scholar]
  17. Maguire M. H., Michal F. Powerful new aggregator of blood platelets--2-chloroadenosine-5'-diphosphate. Nature. 1968 Feb 10;217(5128):571–573. doi: 10.1038/217571a0. [DOI] [PubMed] [Google Scholar]
  18. Mannucci P. M., Sharp A. A. Platelet volume and shape in relation to aggregation and adhesion. Br J Haematol. 1967 Jul;13(4):604–617. doi: 10.1111/j.1365-2141.1967.tb00768.x. [DOI] [PubMed] [Google Scholar]
  19. McLean J. R., Veloso H. Change of shape without aggregation caused by ADP in rabbit platelets at low pH. Life Sci. 1967 Sep 15;6(18):1983–1986. doi: 10.1016/0024-3205(67)90259-7. [DOI] [PubMed] [Google Scholar]
  20. Mills D. C., Roberts G. C. Effects of adrenaline on human blood platelets. J Physiol. 1967 Nov;193(2):443–453. doi: 10.1113/jphysiol.1967.sp008369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. O'Brien J. R., Heywood J. B. Effect of aggregating agents and their inhibitors on the mean platelet shape. J Clin Pathol. 1966 Mar;19(2):148–153. doi: 10.1136/jcp.19.2.148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Pringle J. W. Mechano-chemical transformation in striated muscle. Symp Soc Exp Biol. 1968;22:67–86. [PubMed] [Google Scholar]
  23. Sattin A., Rall T. W. The effect of adenosine and adenine nucleotides on the cyclic adenosine 3', 5'-phosphate content of guinea pig cerebral cortex slices. Mol Pharmacol. 1970 Jan;6(1):13–23. [PubMed] [Google Scholar]
  24. Silver M. D. Cytoplasmic microtubules in rabbit platelets. Z Zellforsch Mikrosk Anat. 1965 Nov 15;68(4):474–480. doi: 10.1007/BF00347711. [DOI] [PubMed] [Google Scholar]
  25. Szent-Györgyi A. G. The role of actin-myosin interaction in contraction. Symp Soc Exp Biol. 1968;22:17–42. [PubMed] [Google Scholar]
  26. Voth D., Schäfer A. Vergleichende photometriche und elektronmikroskopische Untersuchungen an isolierten Rattenhirn-Mitochondrien und-Mikrosomen in vitro unter den bedingungen der osmotischen schwellung und ATP-induzierten Kontraktion. Brain Res. 1968 Sep;10(3):322–341. doi: 10.1016/0006-8993(68)90203-5. [DOI] [PubMed] [Google Scholar]
  27. Wolfe S. M., Shulman N. R. Adenyl cyclase activity in human platelets. Biochem Biophys Res Commun. 1969 Apr 29;35(2):265–272. doi: 10.1016/0006-291x(69)90277-0. [DOI] [PubMed] [Google Scholar]
  28. ZUCKER M. B., BORRELLI J. Reversible alterations in platelet morphology produced by anticoagulants and by cold. Blood. 1954 Jun;9(6):602–608. [PubMed] [Google Scholar]
  29. Zieve P. D., Greenough W. B., 3rd Adenyl cyclase in human platelets: activity and responsiveness. Biochem Biophys Res Commun. 1969 May 22;35(4):462–466. doi: 10.1016/0006-291x(69)90368-4. [DOI] [PubMed] [Google Scholar]

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