Skip to main content
PMC home page
British Journal of Clinical Pharmacology logoLink to British Journal of Clinical Pharmacology
. 1981 Jan;11(1):85–88. doi: 10.1111/j.1365-2125.1981.tb01107.x

Radioimmunoassay for perphenazine in human plasma.

K K Midha, C Mackonka, J K Cooper, J W Hubbard, P K Yeung
PMCID: PMC1401697  PMID: 7213513

Abstract

1 A new sensitive, specific and rapid radioimmunoassay procedure for the determination of plasma concentrations of the neuroleptic drug perphenazine is described. 2 The antiserum developed for perphenazine did not cross-react with most of the major metabolites of perphenazine nor the tricyclic antidepressants and antianxiety agents commonly co-administered with the drug. 3 The assay, based on the above antiserum, enabled the quantitation of 50 pg of the drug in 200 microliters of plasma with a coefficient of variation of about 8% and therefore should be applicable for single dose pharmacokinetic studies, as well as therapeutic monitoring of the drug in patients.

Full text

PDF
87

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abraham G. E. Solid-phase radioimmunoassay of estradiol-17 beta. J Clin Endocrinol Metab. 1969 Jun;29(6):866–870. doi: 10.1210/jcem-29-6-866. [DOI] [PubMed] [Google Scholar]
  2. Breyer U., Gaertner H. J., Prox A. Formation of identical metabolites from piperazine- and dimethylamino-substituted phenothiazine drugs in man, rat and dog. Biochem Pharmacol. 1974 Jan 15;23(2):313–322. doi: 10.1016/0006-2952(74)90422-5. [DOI] [PubMed] [Google Scholar]
  3. Cooper S., Albert J. M., Dugal R., Bertrand M., Elie R. Gas chromatographic determination of amitriptyline, nortriptyline and perphenazine in plasma of schizophrenic patients after administration of the combination of amitriptyline with perphenazine. Arzneimittelforschung. 1979;29(1):158–161. [PubMed] [Google Scholar]
  4. ERLANGER B. F., BOREK F., BEISER S. M., LIEBERMAN S. Steroid-protein conjugates. I. Preparation and characterization of conjugates of bovine serum albumin with testosterone and with cortisone. J Biol Chem. 1957 Oct;228(2):713–727. [PubMed] [Google Scholar]
  5. ERLANGER B. F., BOREK F., BEISER S. M., LIEBERMAN S. Steroid-protein conjugates. II. Preparation and characterization of conjugates of bovine serum albumin with progesterone, deoxycorticosterone, and estrone. J Biol Chem. 1959 May;234(5):1090–1094. [PubMed] [Google Scholar]
  6. Eggert Hansen C., Rosted Christensen T., Elley J., Bolvig Hansen L., Kragh-Sorensen P., Larsen N. E., Naestoft J., Hvidberg E. F. Clinical pharmacokinetic studies of perphenazine. Br J Clin Pharmacol. 1976 Oct;3(5):915–923. doi: 10.1111/j.1365-2125.1976.tb00647.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hansen L. B., Larsen N. E. Plasma concentrations of perphenazine and its sulphoxide metabolite during continuous oral treatment. Psychopharmacology (Berl) 1977 Jul 18;53(2):127–130. doi: 10.1007/BF00426481. [DOI] [PubMed] [Google Scholar]
  8. Jørgensen A. A sensitive and specific radioimmunoassay for cis (Z)-flupenthixol in human serum. Life Sci. 1978 Oct 16;23(15):1533–1542. doi: 10.1016/0024-3205(78)90580-5. [DOI] [PubMed] [Google Scholar]
  9. Kawashima K., Dixon R., Spector S. Development of radioimmunoassay for chlorpromazine. Eur J Pharmacol. 1975 Jun-Jul;32(02):195–202. doi: 10.1016/0014-2999(75)90283-6. [DOI] [PubMed] [Google Scholar]
  10. Larsen N. E., Naestoft J. Determination of perphenazine and fluphenazine in whole blood by gas chromatography. Med Lab Technol. 1973 Apr;30(2):129–132. [PubMed] [Google Scholar]
  11. Larsen N. E., Naestoft J. Determination of perphenazine and its sulphoxide metabolite in human plasma after therapeutic doses by gas chromatography. J Chromatogr. 1975 Jun 18;109(2):259–264. doi: 10.1016/s0021-9673(01)91797-2. [DOI] [PubMed] [Google Scholar]
  12. Midha K. K., Loo J. C., Hubbard J. W., Rowe M. L., McGilveray I. J. Radioimmunoassay for chlorpromazine in plasma. Clin Chem. 1979 Jan;25(1):166–168. [PubMed] [Google Scholar]
  13. Tjaden U. R., Lankelma J., Poppe H. Anodic coulometric detection with a glassy carbon electrode in combination with reversed-phase high-performance liquid chromatography. Determination of blood levels of perphenazine and fluphenazine. J Chromatogr. 1976 Sep 29;125(1):275–286. doi: 10.1016/s0021-9673(00)93825-1. [DOI] [PubMed] [Google Scholar]
  14. Wiles D. H., Franklin M. Radioimmunoassay for fluphenazine in human plasma. Br J Clin Pharmacol. 1978 Mar;5(3):265–268. doi: 10.1111/j.1365-2125.1978.tb01636.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from British Journal of Clinical Pharmacology are provided here courtesy of British Pharmacological Society

RESOURCES