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. 1972 Mar;22(3):475–484.

Measurement of antibody-producing capacity in man

V. Immunoglobulin classes of antibodies to flagellin

Merrill J Rowley, R Wistar, I R Mackay
PMCID: PMC1407786  PMID: 4623760

Abstract

The immunoglobulin classes of antibody to flagellin were determined in twenty-eight persons injected with flagellin from Salmonella adelaide. Sera were examined from non-immunized persons and from persons given primary and secondary immunization. Results obtained by titration before and after treatment of whole serum with 2-mercaptoethanol (ME) were compared with those obtained after fractionation of serum by gel filtration on Sephadex G-200 and by radio-immunoelectrophoresis (RIEP). The ME procedure, whilst not fully reliable for determining class of specific antibody in individual sera, is suitable for routine analyses of many sera. After gel filtration, ME-sensitive antibodies were mostly contained in the IgM protein peak whereas ME-resistant antibodies were mainly associated with the IgG protein peak. Using RIEP, specific antigen binding to the IgM, IgG and IgA precipitin lines was demonstrated. Binding of flagellin to the IgM precipitin line correlated with the presence in whole serum of ME-sensitive antibody and binding to the IgG line with ME-resistant antibody. Antigen binding to the IgA line did not correlate with results from ME-sensitivity studies using whole sera.

Natural antibody in serum before immunization was entirely IgM. The antibody after primary immunization was mainly IgM but varying amounts of IgG were also produced; a change to the synthesis of entirely IgG antibody during the primary response was rare. The antibody after secondary immunization was mostly IgG; there was no evidence of increased IgM synthesis. IgA antibodies to flagellin were detected during both primary and secondary responses in about half of the subjects tested, but were not quantified.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADA G. L., NOSSAL G. J., PYE J. ANTIGENS IN IMMUNITY. III. DISTRIBUTION OF IODINATED ANTIGENS FOLLOWING INJECTION INTO RATS VIA THE HIND FOOTPADS. Aust J Exp Biol Med Sci. 1964 Jun;42:295–310. [PubMed] [Google Scholar]
  2. Bandilla K. K., McDuffie F. C., Gleich G. J. Immunoglobulin classes of antibodies produced in the primary and secondary responses in man. Clin Exp Immunol. 1969 Dec;5(6):627–641. [PMC free article] [PubMed] [Google Scholar]
  3. CONE L., UHR J. W. IMMUNOLOGICAL DEFICIENCY DISORDERS ASSOCIATED WITH CHRONIC LYMPHOCYTIC LEUKEMIA AND MULTIPLE MYELOMA. J Clin Invest. 1964 Dec;43:2241–2248. doi: 10.1172/JCI105098. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cohen I. R., Norins L. C. Antibiodies of the IgG, IgM, and IgA classes in newborn and adult sera reactive with gram-negative bacteria. J Clin Invest. 1968 May;47(5):1053–1062. doi: 10.1172/JCI105795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gleich G. J., Uhr J. W., Vaughan J. H., Swedlund H. A. Antibody formation in dysgammaglobulinemia. J Clin Invest. 1966 Aug;45(8):1334–1340. doi: 10.1172/JCI105440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LOSPALLUTO J., MILLER W., Jr, DORWARD B., FINK C. W. The formation of macroglobulin antibodies. I. Studies on adult humans. J Clin Invest. 1962 Jul;41:1415–1421. doi: 10.1172/JCI104596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lee A. K., Mackay I. R., Rowley M. J., Yap C. Y. Measurement of antibody-producing capacity to flagellin in man. IV. Studies in autoimmune disease, allergy and after azathioprine treatment. Clin Exp Immunol. 1971 Oct;9(4):507–518. [PMC free article] [PubMed] [Google Scholar]
  8. Lee A. K., Mackay I. R., Rowley M. Measurement of antibody-producing capacity in man. 3. The response to flagellin from Salmonella adelaide in chronic diseases. Int Arch Allergy Appl Immunol. 1971;41(2):358–369. doi: 10.1159/000230531. [DOI] [PubMed] [Google Scholar]
  9. Lee A. K., Rowley M., Mackay I. R. Antibody-producing capacity in human cancer. Br J Cancer. 1970 Sep;24(3):454–463. doi: 10.1038/bjc.1970.54. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MICHAEL J. G., ROSEN F. S. ASSOCIATION OF "NATURAL" ANTIBODIES TO GRAM-NEGATIVE BACTERIA WITH THE GAMMA-1-MACROGLOBULINS. J Exp Med. 1963 Oct 1;118:619–626. doi: 10.1084/jem.118.4.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. NOSSAL G. J., ADA G. L., AUSTIN C. M. ANTIGENS IN IMMUNITY. II. IMMUNOGENIC PROPERTIES OF FLAGELLA, POLYMERIZED FLAGELLIN AND FLAGELLIN IN THE PRIMARY RESPONSE. Aust J Exp Biol Med Sci. 1964 Jun;42:283–294. [PubMed] [Google Scholar]
  12. Newcomb R. W., Ishizaka K. Human diphtheria antitoxin in immunoglobulin classes IgG and IgA. J Immunol. 1967 Jul;99(1):40–48. [PubMed] [Google Scholar]
  13. Rowley M. J. "Natural" antibody in man to flagellar antigens of Salmonella adelaide. Aust J Exp Biol Med Sci. 1970 Apr;48(2):249–252. doi: 10.1038/icb.1970.26. [DOI] [PubMed] [Google Scholar]
  14. Rowley M. J., Mackay I. R., McKenzie I. F. Antibody production in immunosuppressed recipients of renal allografts. Lancet. 1969 Oct 4;2(7623):708–710. doi: 10.1016/s0140-6736(69)90425-5. [DOI] [PubMed] [Google Scholar]
  15. SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]
  16. Southam C. M. The immunologic status of patients with nonlymphomatous cancer. Cancer Res. 1968 Jul;28(7):1433–1440. [PubMed] [Google Scholar]
  17. Swanson M. A., Schwartz R. S. Immunosuppressive therapy. The relation between clinical response and immunologic competence. N Engl J Med. 1967 Jul 27;277(4):163–170. doi: 10.1056/NEJM196707272770401. [DOI] [PubMed] [Google Scholar]
  18. UHR J. W., DANCIS J., FRANKLIN E. C., FINKELSTEIN M. S., LEWIS E. W. The antibody response to bacteriophage phi-X 174 in newborn premature infants. J Clin Invest. 1962 Jul;41:1509–1513. doi: 10.1172/JCI104606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wistar R., Jr Serum antibody to Salmonella flagellar antigens. I. Methods of antibody assay. Aust J Exp Biol Med Sci. 1968 Dec;46(6):769–777. doi: 10.1038/icb.1968.183. [DOI] [PubMed] [Google Scholar]

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