Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1972 Mar;22(3):319–331.

Specific antibody-mediated effect on the immune response

suppression and augmentation of the primary immune response in mice by different classes of antibodies

R A Murgita, S I Vas
PMCID: PMC1407808  PMID: 4554743

Abstract

The effect of prior administration of two classes of 7S mouse anti-sheep red blood cell (SRBC) antibodies on the primary immune response to SRBC was studied. Mouse γG1 and γG2 immunoglobulins were isolated from serum by zone electrophoresis and density gradient isolectric focusing. The immunoglobulins were defined by qualitative and quantitative studies, and their effects on the primary response were determined by administering doses ranging from 0.5 to 0.001 mg of immunoglobulin 2 hours before injection of antigen. Gamma G1 antibody suppressed the response to SRBC at all concentrations. High doses of γG2 antibody partially suppressed 19S plaque-forming cells (PFC), but had no significant effect on serum haemagglutinin (HA) levels. Low doses of γG2 antibody specifically augmented both the 19S PFC and serum HA levels. It is concluded that the specific suppressing and augmenting influences of antibodies on the primary response are a function of class. In addition, it is proposed that γG1 and γG2 antibodies can act as specific regulatory elements during the primary response by exerting these two competing biological effects on antibody synthesis.

Full text

PDF
322

Images in this article

323FIG. 2
on p.323

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chan P. L., Sinclair N. R. Regulation of the immune response. V. An analysis of the function of the Fc portion of antibody in suppression of an immune response with respect to interaction with components of the lymphoid system. Immunology. 1971 Dec;21(6):967–981. [PMC free article] [PubMed] [Google Scholar]
  2. Dennert G. The mechanism of antibody-induced stimulation and inhibition of the immune response. J Immunol. 1971 Apr;106(4):951–955. [PubMed] [Google Scholar]
  3. FAHEY J. L., MCKELVEY E. M. QUANTITATIVE DETERMINATION OF SERUM IMMUNOGLOBULINS IN ANTIBODY-AGAR PLATES. J Immunol. 1965 Jan;94:84–90. [PubMed] [Google Scholar]
  4. Feldmann M., Diener E. Antibody-mediated suppression of the immune response in vitro. I. Evidence for a central effect. J Exp Med. 1970 Feb;131(2):247–274. doi: 10.1084/jem.131.2.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. MOLLER G., WIGZELL H. ANTIBODY SYNTHESIS AT THE CELLULAR LEVEL. ANTIBODY-INDUCED SUPPRESSION OF 19S AND 7S ANTIBODY RESPONSE. J Exp Med. 1965 Jun 1;121:969–989. doi: 10.1084/jem.121.6.969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Murgita R. A., Tokuda S. The specific suppression of the early immune response in mice by "slow" and "fast" migrating 7S mouse antibody fractions. Life Sci. 1967 Oct 15;6(20):2185–2189. doi: 10.1016/0024-3205(67)90241-x. [DOI] [PubMed] [Google Scholar]
  7. Murgita R. A., Vas S. I. Isoelectric separation of mouse immunoglobulins. J Immunol. 1970 Feb;104(2):514–517. [PubMed] [Google Scholar]
  8. Nash D. R., Heremans J. F. A quantitative antibody-binding method for the determination of specific antibody within different immunoglobulin classes. Application to four Ig classes in the mouse. Immunology. 1969 Nov;17(5):685–694. [PMC free article] [PubMed] [Google Scholar]
  9. Nicholas R., Sinclair S. C. Regulation of the immune response. I. Reduction in ability of specific antibody to inhibit long-lasting IgG immunological priming after removal of the Fc fragment. J Exp Med. 1969 Jun 1;129(6):1183–1201. doi: 10.1084/jem.129.6.1183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. OVARY Z., BARTH W. F., FAHEY J. L. THE IMMUNOGLOBULINS OF MICE. 3. SKIN SENSITIZING ACTIVITY OF MOUSE IMMUNOGLOBULINS. J Immunol. 1965 Mar;94:410–415. [PubMed] [Google Scholar]
  11. Pierce C. W. Immune responses in vitro. II. Suppression of the immune response in vitro by specific antibody. J Exp Med. 1969 Aug 1;130(2):365–379. doi: 10.1084/jem.130.2.365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rowley D. A., Fitch F. W., Axelrad M. A., Pierce C. W. The immune response suppressed by specific antibody. Immunology. 1969 Apr;16(4):549–559. [PMC free article] [PubMed] [Google Scholar]
  13. Ryder R. J., Schwartz R. S. Immunosuppression by antibody: localization of site of action. J Immunol. 1969 Nov;103(5):970–978. [PubMed] [Google Scholar]
  14. Siskind G. W., Paul W. E., Benacerraf B. Studies on the effect of the carrier molecule on antihapten antibody synthesis. I. Effect of carrier on the nature of the antibody synthesized. J Exp Med. 1966 Apr 1;123(4):673–688. doi: 10.1084/jem.123.4.673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Takasugi M., Hildemann W. H. Regulation of immunity toward allogeneic tumors in mice. I. Effect of antiserum fractions on tumor growth. J Natl Cancer Inst. 1969 Oct;43(4):843–856. [PubMed] [Google Scholar]
  16. Tokuda S., McEntee F. Immunologic enhancement of sarcoma I by mouse gamma-globulin fractions. Transplantation. 1967 Jul;5(4):606–614. doi: 10.1097/00007890-196707000-00005. [DOI] [PubMed] [Google Scholar]
  17. Uhr J. W., Möller G. Regulatory effect of antibody on the immune response. Adv Immunol. 1968;8:81–127. doi: 10.1016/s0065-2776(08)60465-4. [DOI] [PubMed] [Google Scholar]
  18. Wigzell H. Antibody synthesis at the cellular level. Antibody-induced suppression of 7S antibody synthesis. J Exp Med. 1966 Nov 1;124(5):953–969. doi: 10.1084/jem.124.5.953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wortis H. H., Taylor R. B., Dresser D. W. Antibody production studied by means of the localized haemolysis in gel (LHG) assay. II. Assay procedure. Immunology. 1968 Jan;14(1):69–79. [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES