Abstract
Buoyant density centrifugation of linear albumin gradients was used to fractionate suspensions of guinea-pig spleen cells. The fractions obtained were studied with respect to morphology and function of their cells. Using this technique it was shown that cells responsible for the production of sheep cell haemolysin were located in fractions distinct from those which could be used for the passive transfer of delayed tuberculoprotein hypersensitivity.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dicke K. A., Tridente G., van Bekkum D. W. The selective elimination of immunologically competent cells from bone marrow and lymphocyte cell mixtures. 3. In vitro test for detection of immunocompetent cells in fractionated mouse spleen cell suspensions and primate bone marrow suspensions. Transplantation. 1969 Oct;8(4):422–434. doi: 10.1097/00007890-196910000-00014. [DOI] [PubMed] [Google Scholar]
- Gorczynski R. M., Miller R. G., Phillips R. A. Homogeneity of antibody-producing cells as analysed by their buoyant density in gradients of Ficoll. Immunology. 1970 Nov;19(5):817–829. [PMC free article] [PubMed] [Google Scholar]
- Haskill J. S. Density distribution analysis of antigen-sensitive cells in the rat. J Exp Med. 1969 Oct 1;130(4):877–893. doi: 10.1084/jem.130.4.877. [DOI] [PMC free article] [PubMed] [Google Scholar]
- L'age-Stehr J., Herzenberg L. A. Immunological memory in mice. I. Physical separation and partial characterization of memory cells for different immunoglobulin classes from each other and from antibody-producing cells. J Exp Med. 1970 Jun 1;131(6):1093–1108. doi: 10.1084/jem.131.6.1093. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Möller G., Hiesche K. Fractionation of immunocompetent spleen cells by albumin density gradient centrifugation. Immunology. 1970 Apr;18(4):585–594. [PMC free article] [PubMed] [Google Scholar]
- Shortman K. The separation of different cell classes from lymphoid organs. 1. The use of glass bead columns to separate small lymphocytes, remove damaged cells and fractionate cell suspensions. Aust J Exp Biol Med Sci. 1966 Jun;44(3):271–286. [PubMed] [Google Scholar]
- Shortman K. The separation of different cell classes from lymphoid organs. II. The purification and analysis of lymphocyte populations by equilibrium density gradient centrifugation. Aust J Exp Biol Med Sci. 1968 Aug;46(4):375–396. doi: 10.1038/icb.1968.32. [DOI] [PubMed] [Google Scholar]
- Sundaram K., Phondke G. P., Sundaresan P. In vitro studies on antibody production by lymph node cells using cell electrophoresis. Immunology. 1967 Oct;13(4):433–439. [PMC free article] [PubMed] [Google Scholar]