Abstract
Interaction of normal and Salmonella typhi `H'-sensitized rat lymph node cells with two different types of anti-lymphocyte serum (ALS) has been studied. One type of ALS, named as ALS(N), was prepared by immunizing rabbits with normal non-sensitized lymph node cells while the other, ALS(I), was produced by injecting rabbits with sensitized cells.
The histogram of electrophoretic mobilities (EPM) of ALS(N)-treated normal cells resolves into two Gaussian components corresponding to Class `A' and Class `B' cells respectively. ALS(N) brings about a significant reduction in the mean EPM of both these components indicating that ALS(N) binds to both the functional cell types with equal avidity. Analysis of the histogram of EPM of ALS(N)-treated sensitized cells reveals that ALS(N) leaves the mean EPM of antibody-producing Class `C' cells unchanged. ALS(I), on the other hand, is effective in binding to Class `C' cells in addition to the other cell types. These investigations thus demonstrate that ALS(N) has a differential action on the antigen-sensitive cells as compared to the antibody-producing cells. Further, this lack of interaction with Class `C' cells appears to be a result of a radically different surface-antigenic configuration of these cells.
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Selected References
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- Barth R. F. Studies on heterologous antilymphocyte and antithymocyte sera. IV. Further evidence for a preferential effect on antigen-sensitive cells. J Immunol. 1969 Oct;103(4):648–654. [PubMed] [Google Scholar]
- James K. Effect of antilymphocyte antibody on humoral antibody formation. Fed Proc. 1970 Jan-Feb;29(1):160–166. [PubMed] [Google Scholar]
- Lance E. M. Mode of action of antilymphocyte serum. Fed Proc. 1970 Jan-Feb;29(1):209–211. [PubMed] [Google Scholar]
- Lance E. Erasure of immunological memory with anti-lymphocyte serum. Nature. 1968 Feb 10;217(5128):557–558. doi: 10.1038/217557a0. [DOI] [PubMed] [Google Scholar]
- Levey R. H. Influences of antilymphocyte serum on cell-mediated and antibody-mediated responses. Fed Proc. 1970 Jan-Feb;29(1):156–158. [PubMed] [Google Scholar]
- Levey R. H., Medawar P. B. Nature and mode of action of antilymphocytic antiserum. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1130–1137. doi: 10.1073/pnas.56.4.1130. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Martin W. J., Miller J. F. Cell to cell interaction in the immune response. IV. Site of action of antilymphocyte globulin. J Exp Med. 1968 Oct 1;128(4):855–874. doi: 10.1084/jem.128.4.855. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Monaco A. P., Wood M. L., Gray J. G., Russell P. S. Studies on heterologous anti-lymphocyte serum in mice. II. Effect on the immune response. J Immunol. 1966 Feb;96(2):229–238. [PubMed] [Google Scholar]
- Möller G., Zukoski C. Differential effect of heterologous anti-lymphocyte serum on antibody-producing cells and antigen-sensitive cells. J Immunol. 1968 Aug;101(2):325–332. [PubMed] [Google Scholar]
- Phondke G. P., Sundaram K. Cell electrophoretic studies on lymph node cells--possible existence of two populations. Immunology. 1971 Jul;21(1):1–9. [PMC free article] [PubMed] [Google Scholar]
- Phondke G. P., Sundaresan P., Sundaram K. Quantitative in vitro titration of anti-lymphocyte serum. Indian J Exp Biol. 1970 Oct;8(4):329–330. [PubMed] [Google Scholar]
- Sundaram K., Phondke G. P., Ambrose E. J. Electrophoretic mobilities of antigen-stimulated lymph node cells. Immunology. 1967 Jan;12(1):21–26. [PMC free article] [PubMed] [Google Scholar]
- Sundaram K., Phondke G. P., Sundaresan P. In vitro studies on antibody production by lymph node cells using cell electrophoresis. Immunology. 1967 Oct;13(4):433–439. [PMC free article] [PubMed] [Google Scholar]
- Taub R. N., Lance E. M. Histopathological effects in mice of heterologous antilymphocyte serum. J Exp Med. 1968 Dec 1;128(6):1281–1307. doi: 10.1084/jem.128.6.1281. [DOI] [PMC free article] [PubMed] [Google Scholar]