Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1972 Feb;22(2):199–210.

The cellular transfer of immunity to Nippostrongylus brasiliensis in inbred rats (Lewis strain)

J D Kelly, J K Dineen
PMCID: PMC1408193  PMID: 5062015

Abstract

Mesenteric lymph node cells obtained from highly inbred donor rats (Lewis strain), resistant to Nippostrongylus brasiliensis infection, were syngeneically transferred by intravenous injection into previously uninfected recipients. The adoptively immunized recipients were then challenged with either 1500 or 3000 third stage N. brasiliensis larvae on the day of cell transfer. The degree of resistance transferred was assessed by monitoring daily faecal egg output, differential worm burdens on days 6 and 10 of infection and the number of eggs per uterus in gravid worms.

The syngeneic transfer of 100 × 106 immune mesenteric lymph node cells invariably resulted in suppression of egg production, a two- to four-fold reduction in the number of eggs per uterus in gravid females and rejection of at least 75 per cent of adult worms by days 6 and 10 of infection.

It was also noted that mesenteric lymph node cells obtained from donors on day 15 of a primary infection were more effective than those obtained from donors immunized by multiple infections.

Immune cells transferred from donors on day 4 of infection were equally effective with those transferred on day 0. However, immune cells transferred on or after day 10 of infection had little or no effect and this shows that the parasite is less susceptible to an attack mounted by the transferred cells during the later stages of infection.

Full text

PDF
200

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AGLAND B. M., DINEEN J. K. THE CELLULAR TRANSFER OF IMMUNITY TO TRICHOSTRONGYLUS COLUBRIFORMIS IN AN ISOGENIC STRAIN OF GUINES-PIG. Aust J Exp Biol Med Sci. 1965 Jul;43:429–438. doi: 10.1038/icb.1965.77. [DOI] [PubMed] [Google Scholar]
  2. Dhar D. N., Singh K. S. Attempt at passive immunization against the nematode, Oesophagostomum columbianum. Indian J Exp Biol. 1970 Apr;8(2):149–150. [PubMed] [Google Scholar]
  3. Dineen J. K., Wagland B. M., Ronai P. M. The cellular transfer of immunity to Trichostrongylus colubriformis in an isogenic strain of guinea-pig. 3. The localization and functional activity of immune lymph node cells following syngeneic and allogeneic transfer. Immunology. 1968 Sep;15(3):335–341. [PMC free article] [PubMed] [Google Scholar]
  4. Dineen J. K., Wagland B. M. The cellular transfer of immunity to Trichostrongylus colubriformis in an isogenic strain of guinea-pig. II. The relative susceptibility of the larval and adult stages of the parasite to immunological attack. Immunology. 1966 Jul;11(1):47–57. [PMC free article] [PubMed] [Google Scholar]
  5. Friedberg W., Neas B. R., Faulkner D. N., Friedberg M. H. Immunity to Hymenolepis nana: transfer by spleen cells. J Parasitol. 1967 Aug;53(4):895–896. [PubMed] [Google Scholar]
  6. Friedberg W., Neas B. R., Friedberg M. H., Faulkner D. N. Syngeneic marrow graft: effect on persistence of preirradiation immunity to Hymenolepis nana in mice. Proc Soc Exp Biol Med. 1967 Mar;124(3):792–793. doi: 10.3181/00379727-124-31854. [DOI] [PubMed] [Google Scholar]
  7. HUNTER G. C., LEIGH L. C. Studies on the resistance of rats to the nematode Nippostrongylus muris (Yokogawa, 1920). III. Attempt to transfer immunity adoptively. Parasitology. 1961 Nov;51:357–366. doi: 10.1017/s0031182000070621. [DOI] [PubMed] [Google Scholar]
  8. Jarrett E. E., Jarrett W. F., Urquhart G. M. Quantitative studies on the kinetics of establishment and expulsion of intestinal nematode populations in susceptible and immune hosts. Nippostrongylus brasiliensis in the rat. Parasitology. 1968 Aug;58(3):625–639. doi: 10.1017/s0031182000028924. [DOI] [PubMed] [Google Scholar]
  9. Jarrett E. E., Urquhart G. M., Douthwaite R. M. Immunological unresponsiveness to helminth parasites. II. Antibody response and hypersensitivity reaction associated with Nippostrongylus brasiliensis infection in young rats. Exp Parasitol. 1969 Apr;24(2):270–278. doi: 10.1016/0014-4894(69)90165-9. [DOI] [PubMed] [Google Scholar]
  10. Jones V. E., Edwards A. J., Ogilvie B. M. The circulating immunoglobulins involved in protective immunity to the intestinal stage of Nippostrongylus brasiliensis in the rat. Immunology. 1970 May;18(5):621–633. [PMC free article] [PubMed] [Google Scholar]
  11. Kassai T., Szepes G. Experiments on adoptive transfer of immunological tolerance and of immunity to Nippostrongylus brasiliensis in rats. Acta Vet Acad Sci Hung. 1970;20(2):207–217. [PubMed] [Google Scholar]
  12. LARSH J. E., Jr, GOULSON H. T., WEATHERLY N. F. STUDIES ON DELAYED (CELLULAR) HYPERSENSITIVITY IN MICE INFECTED WITH TRICHINELLA SPIRALIS. II. TRANSFER OF PERITONEAL EXUDATE CELLS. J Parasitol. 1964 Aug;50:496–498. [PubMed] [Google Scholar]
  13. Lang B. Z. Host-parasite relationships of Fasciola hepatica in the white mouse. II. Studies on acquired immunity. J Parasitol. 1967 Feb;53(1):21–30. [PubMed] [Google Scholar]
  14. Lang B. Z., Larsh J. E., Jr, Weatherly N. F., Goulson H. T. Demonstration of immunity to Fasciola hepatica in recipient mice given peritoneal exudate cells. J Parasitol. 1967 Feb;53(1):208–209. [PubMed] [Google Scholar]
  15. MULLIGAN W., URQUHART G. M., JENNINGS F. W., NEILSON J. T. IMMUNOLOGICAL STUDIES ON NIPPOSTRONGYLUS BRASILIENSIS INFECTION IN THE RAT: "SELF-CURE" PHENOMENON. Exp Parasitol. 1965 Jun;16:341–347. doi: 10.1016/0014-4894(65)90056-1. [DOI] [PubMed] [Google Scholar]
  16. Ogilvie B. M., Jones V. E. Passive protection with cells or antiserum against Nippostronglylus brasiliensis in the rat. Parasitology. 1968 Nov;58(4):939–949. doi: 10.1017/s0031182000069705. [DOI] [PubMed] [Google Scholar]
  17. Okamoto K. Hymenolepis nana: depression and restoration of acquired immunity in neonatally thymectomized mice. Exp Parasitol. 1970 Feb;27(1):28–32. doi: 10.1016/s0014-4894(70)80006-6. [DOI] [PubMed] [Google Scholar]
  18. Panter H. C. The mechanism of immunity of mice to Nematospiroides dubius. J Parasitol. 1969 Feb;55(1):38–43. [PubMed] [Google Scholar]
  19. THORSON R. E. Absorption of protective antibodies from serum of rats immune to the nematode, Nippostrongylus muris. J Parasitol. 1954 Jun;40(3):300–303. [PubMed] [Google Scholar]
  20. THORSON R. E. Studies on the mechanism of immunity in the rat to the nematode, Nippostrongylus muris. Am J Hyg. 1953 Jul;58(1):1–15. doi: 10.1093/oxfordjournals.aje.a119584. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES