Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1971 Dec;21(6):1065–1071.

Molecular weight estimation of immunoglobulin subunits on polyacrylamide gel

C Ahmad-Zadeh, J D Piguet, Lorenza Colli
PMCID: PMC1408247  PMID: 5167037

Abstract

Molecular weights of immunoglobulin polypeptide chains can be accurately determined by dodecyl sulphate polyacrylamide gel electrophoresis. Non-alkylated immunoglobulin preparations can be used. Depending on the mobility of the polypeptide chain, a relatively narrow range of standard size should be used to calibrate the molecular weight plots; this is concluded from an evaluation of the molecular weight-mobility relationship of dodecyl sulphate-complexes of proteins in 10 per cent polyacrylamide gel.

Full text

PDF

Images in this article

1068FIG. 1
on p.1068

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avrameas S., Ternynck T. The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry. 1969 Jan;6(1):53–66. doi: 10.1016/0019-2791(69)90178-5. [DOI] [PubMed] [Google Scholar]
  2. Black L. W. Ahmad-Zadeh C,+AHMADAAZADEH C: Internal proteins of bacteriophage T4D: their characterization and relation to head structure and assembly. J Mol Biol. 1971 Apr 14;57(1):71–92. doi: 10.1016/0022-2836(71)90120-3. [DOI] [PubMed] [Google Scholar]
  3. Buxbaum J., Franklin E. C., Scharff M. D. Immunoglobulin M heavy chain disease: intracellular origin of the mu chain fragment. Science. 1970 Aug 21;169(3947):770–773. doi: 10.1126/science.169.3947.770. [DOI] [PubMed] [Google Scholar]
  4. Dickson R. C., Barnes S. L., Eiserling F. A. Structural proteins of bacteriophage T4. J Mol Biol. 1970 Nov 14;53(3):461–474. doi: 10.1016/0022-2836(70)90077-x. [DOI] [PubMed] [Google Scholar]
  5. Dorrington K. J., Tanford C. Molecular size and conformation of immunoglobulins. Adv Immunol. 1970;12:333–381. doi: 10.1016/s0065-2776(08)60173-x. [DOI] [PubMed] [Google Scholar]
  6. Maizel J. V., Jr Acrylamide-gel electrophorograms by mechanical fractionation: radioactive adenovirus proteins. Science. 1966 Feb 25;151(3713):988–990. doi: 10.1126/science.151.3713.988. [DOI] [PubMed] [Google Scholar]
  7. Maizel J. V., Jr, White D. O., Scharff M. D. The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12. Virology. 1968 Sep;36(1):115–125. doi: 10.1016/0042-6822(68)90121-9. [DOI] [PubMed] [Google Scholar]
  8. Parkhouse R. M., Askonas B. A., Dourmashkin R. R. Electron microscopic studies of mouse immunoglobulin M; structure and reconstitution following reduction. Immunology. 1970 Apr;18(4):575–584. [PMC free article] [PubMed] [Google Scholar]
  9. Reynolds J. A., Tanford C. Binding of dodecyl sulfate to proteins at high binding ratios. Possible implications for the state of proteins in biological membranes. Proc Natl Acad Sci U S A. 1970 Jul;66(3):1002–1007. doi: 10.1073/pnas.66.3.1002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. STRAUSS A. J., KEMP P. G., Jr, VANNIER W. E., GOODMAN H. C. PURIFICATION OF HUMAN SERUM GAMMA-GLOBULIN FOR IMMUNOLOGIC STUDIES: GAMMA-GLOBULIN FRAGMENTATION AFTER SULFATE PRECIPITATION AND PROLONGED DIALYSIS. J Immunol. 1964 Jul;93:24–34. [PubMed] [Google Scholar]
  11. Schubert D. Immunoglobulin biosynthesis. IV. Carbohydrate attachment to immunoglobulin subunits. J Mol Biol. 1970 Jul 28;51(2):287–301. doi: 10.1016/0022-2836(70)90143-9. [DOI] [PubMed] [Google Scholar]
  12. Shapiro A. L., Viñuela E., Maizel J. V., Jr Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967 Sep 7;28(5):815–820. doi: 10.1016/0006-291x(67)90391-9. [DOI] [PubMed] [Google Scholar]
  13. Strauss J. H., Jr, Burge B. W., Darnell J. E. Sindbis virus infection of chick and hamster cells: synthesis of virus-specific proteins. Virology. 1969 Mar;37(3):367–376. doi: 10.1016/0042-6822(69)90220-7. [DOI] [PubMed] [Google Scholar]
  14. Traut R. R., Delius H., Ahmad-Zadeh C., Bickle T. A., Pearson P., Tissières A. Ribosomal proteins of E. Coli: stoichiometry and implications for ribosome structure. Cold Spring Harb Symp Quant Biol. 1969;34:25–38. doi: 10.1101/sqb.1969.034.01.007. [DOI] [PubMed] [Google Scholar]
  15. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES