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. 1971 Dec;21(6):1065–1071.

Molecular weight estimation of immunoglobulin subunits on polyacrylamide gel

C Ahmad-Zadeh, J D Piguet, Lorenza Colli
PMCID: PMC1408247  PMID: 5167037

Abstract

Molecular weights of immunoglobulin polypeptide chains can be accurately determined by dodecyl sulphate polyacrylamide gel electrophoresis. Non-alkylated immunoglobulin preparations can be used. Depending on the mobility of the polypeptide chain, a relatively narrow range of standard size should be used to calibrate the molecular weight plots; this is concluded from an evaluation of the molecular weight-mobility relationship of dodecyl sulphate-complexes of proteins in 10 per cent polyacrylamide gel.

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Selected References

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