Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1967 Jan;12(1):21–26.

Electrophoretic mobilities of antigen-stimulated lymph node cells

K Sundaram, G P Phondke, E J Ambrose
PMCID: PMC1409004  PMID: 6017283

Abstract

The effect of immunization against a bacterial antigen on electrophoretic mobility of lymph node cells has been studied.

Incubation of non-stimulated lymph node cells with antigens altered their electrophoretic mobility. However, on washing the antigen away, the original mobility was regained.

Antigen-stimulated lymph node cells had a lower electrophoretic mobility. It was further irreversibly reduced on incubation with the same antigen. This suggests the possibility of formation of firm antigen—antibody complexes at the cell surface.

Implications of these findings and feasibility of using the technique of cell electrophoresis for the study of immune response at the cellular level is discussed.

Full text

PDF
23

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BANGHAM A. D., HEARD D. H., FLEMANS R., SEAMAN G. V. An apparatus for microelectrophoresis of small particles. Nature. 1958 Sep 6;182(4636):642–644. doi: 10.1038/182642a0. [DOI] [PubMed] [Google Scholar]
  2. FORRESTER J. A., AMBROSE E. J., MACPHERSON J. A. Electrophoretic investigations of a clone of hamster fibroblasts and polyoma-transformed cells from the same population. Nature. 1962 Dec 15;196:1068–1070. doi: 10.1038/1961068a0. [DOI] [PubMed] [Google Scholar]
  3. FORRESTER J. A., AMBROSE E. J., STOKER M. G. MICROELECTROPHORESIS OF NORMAL AND TRANSFORMED CLONES OF HAMSTER KIDNEY FIBROBLASTS. Nature. 1964 Feb 29;201:945–946. doi: 10.1038/201945a0. [DOI] [PubMed] [Google Scholar]
  4. FORRESTER J. A., DUMONDE D. C., AMBROSE E. J. THE EFFECTS OF ANTIBODIES ON CELLS. II. CHANGES IN THE ELECTROPHORETIC MOBILITY OF ASCITES TUMOUR CELLS TREATED WITH ANTIBODIES AND COMPLEMENT. Immunology. 1965 Jan;8:37–48. [PMC free article] [PubMed] [Google Scholar]
  5. MAKELA O., NOSSAL G. J. Bacterial adherence: a method for detecting antibody production by single cells. J Immunol. 1961 Oct;87:447–456. [PubMed] [Google Scholar]
  6. NOSSAL G. J. Antibody production by single cells. Br J Exp Pathol. 1958 Oct;39(5):544–551. [PMC free article] [PubMed] [Google Scholar]
  7. NOSSAL G. J., LEDERBERG J. Antibody production by single cells. Nature. 1958 May 17;181(4620):1419–1420. doi: 10.1038/1811419a0. [DOI] [PubMed] [Google Scholar]
  8. REISS E., MERTENS E., EHRICH W. E. Agglutination of bacteria by lymphoid cell in vitro. Proc Soc Exp Biol Med. 1950 Aug;74(4):732–735. doi: 10.3181/00379727-74-18030. [DOI] [PubMed] [Google Scholar]
  9. RUHENSTROTH-BAUER G., STRAUB E., SACHTLEBEN P., FUHRMANN G. F. [Electrophoretic mobility of blood cells in normal and sick persons]. Munch Med Wochenschr. 1961 Apr 14;103:794–797. [PubMed] [Google Scholar]
  10. SEAMAN G. V., SWANK R. L. SURFACE CHARACTERISTICS OF DOG CHYLOMICRA AND SOME LIPID MODELS. J Physiol. 1963 Aug;168:118–128. doi: 10.1113/jphysiol.1963.sp007181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. STEIN G., SEAMAN G. V., HEARD D. H. Action of low doses of ionizing radiations on the electrophoretic mobility of Ehrlich ascites tumour cells. Nature. 1962 Jan 20;193:238–239. doi: 10.1038/193238a0. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES