Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1967 Jul;13(1):63–69.

Immunogenicity of heterologous Fc and Fab immunoglobulin fragments in rabbits, guinea-pigs and rats

R A Binaghi, R Oriol, Yolande Boussac-Aron
PMCID: PMC1409151  PMID: 6027784

Abstract

Rabbits, guinea-pigs and rats were immunized with various heterologous 7S and 19S immunoglobulins from each other and man, and the antisera obtained were studied by immunoelectrophoresis.

Rabbits produced antibodies against the Fc and the Fab fragments of the immunoglobulin injected, while guinea-pigs and rats only produced anti-Fc antibodies.

The fact that guinea-pigs and rats only respond to the specific determinants of each class of immunoglobulin provides a simple method for the preparation of class-specific antisera.

Full text

PDF
69

Images in this article

66FIG. 1
on p.66
67FIG. 2
on p.67

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amkraut A. A., Garvey J. S., Campbell D. H. Competition of haptens. J Exp Med. 1966 Sep 1;124(3):293–306. doi: 10.1084/jem.124.3.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BINAGHI R. A., BENACERRAF B., BLOCH K. J., KOURILSKY F. M. PROPERTIES OF RAT ANAPHYLACTIC ANTIBODY. J Immunol. 1964 Jun;92:927–933. [PubMed] [Google Scholar]
  3. Binaghi R. A. Production of 7 S immunoglobulins in immunized guinea pigs. J Immunol. 1966 Aug;97(2):159–164. [PubMed] [Google Scholar]
  4. Binaghi R. A., Sarandon de Merlo E. Characterization of rat IgA and its non-identity with the anaphylactic antibody. Int Arch Allergy Appl Immunol. 1966;30(6):589–596. doi: 10.1159/000229845. [DOI] [PubMed] [Google Scholar]
  5. FAHEY J. L., MCLAUGHLIN C. PREPARATION OF ANTISERA SPECIFIC FOR 6.6 S GAMMA-GLOBULINS, BETA 2A-GLOBULINS, GAMMA-1.-MACROGLOBULINS, AND FOR TYPE I AND II COMMON GAMMA-GLOBULIN DETERMINANTS. J Immunol. 1963 Oct;91:484–497. [PubMed] [Google Scholar]
  6. HALPERN B. N., PACAUD A. Technique de prèlèvelent d'échantillons de sang chez les petitis animaux de laboratoire par ponction du plexus ophthalmique. C R Seances Soc Biol Fil. 1951 Oct;145(19-20):1465–1466. [PubMed] [Google Scholar]
  7. NISONOFF A., WISSLER F. C., LIPMAN L. N., WOERNLEY D. L. Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulfide bonds. Arch Biochem Biophys. 1960 Aug;89:230–244. doi: 10.1016/0003-9861(60)90049-7. [DOI] [PubMed] [Google Scholar]
  8. OETTGEN H. F., BINAGHI R. A., BENACERRAF B. HEXOSE CONTENT OF GUINEA PIG GAMMA-1 AND GAMMA-2 IMMUNOGLOBULINS. Proc Soc Exp Biol Med. 1965 Feb;118:336–342. [PubMed] [Google Scholar]
  9. Oettgen H. F., Binaghi R. A., Benacerraf B. The effect of various hormones on the serum level of anaphylactic antibody in the rat. Int Arch Allergy Appl Immunol. 1966;29(2):131–139. doi: 10.1159/000229694. [DOI] [PubMed] [Google Scholar]
  10. PORTER R. R. The hydrolysis of rabbit y-globulin and antibodies with crystalline papain. Biochem J. 1959 Sep;73:119–126. doi: 10.1042/bj0730119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES