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. 1967 Jun;12(6):689–699.

Antibodies of IgA and IgG class in normal human urine

M W Turner, D S Rowe
PMCID: PMC1409171  PMID: 4165392

Abstract

Urine collected and concentrated from three individuals after tetanus toxoid immunization has been studied for antibody activity. Gel-filtration on Sephadex G-200 was used to fractionate the urine concentrates and antibody activity in the eluates was assessed by a passive haemagglutination test. Haemagglutinating activity was demonstrated by inhibition experiments in the IgG and IgA classes of immunoglobulin but not in the IgM or IgD classes. In no case could antibody activity be ascribed to molecules smaller than intact IgG.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERGGARD I., EDELMAN G. M. Normal counterparts to Bence-Jones proteins: free L polypeptide chains of human gamma-globulin. Proc Natl Acad Sci U S A. 1963 Mar 15;49:330–337. doi: 10.1073/pnas.49.3.330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DANIEL T. M., WEYAND J. G., Jr, STAVITSKY A. B. MICROMETHODS FOR THE STUDY OF PROTEINS AND ANTIBODIES. IV. FACTORS INVOLVED IN THE PREPARATION AND USE OF A STABLE PREPARATION OF FORMALINIZED, TANNIC ACID-TREATED, PROTEIN-SENSITIZED ERYTHROCYTES FOR DETECTION OF ANTIGEN AND ANTIBODY. J Immunol. 1963 May;90:741–750. [PubMed] [Google Scholar]
  3. FAHEY J. L., MCLAUGHLIN C. PREPARATION OF ANTISERA SPECIFIC FOR 6.6 S GAMMA-GLOBULINS, BETA 2A-GLOBULINS, GAMMA-1.-MACROGLOBULINS, AND FOR TYPE I AND II COMMON GAMMA-GLOBULIN DETERMINANTS. J Immunol. 1963 Oct;91:484–497. [PubMed] [Google Scholar]
  4. GRABAR P., WILLIAMS C. A. Méthode permettant l'étude conjuguée des proprietés électrophorétiques et immunochimiques d'un mélange de protéines; application au sérum sanguin. Biochim Biophys Acta. 1953 Jan;10(1):193–194. doi: 10.1016/0006-3002(53)90233-9. [DOI] [PubMed] [Google Scholar]
  5. HANSON L. A., TAN E. M. CHARACTERIZATION OF ANTIBODIES IN HUMAN URINE. J Clin Invest. 1965 May;44:703–715. doi: 10.1172/JCI105183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. MERLER E., REMINGTON J. S., FINLAND M., GITLIN D. CHARACTERIZATION OF ANTIBODIES IN NORMAL HUMAN URINE. J Clin Invest. 1963 Aug;42:1340–1352. doi: 10.1172/JCI104818. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Merler E. The properties of isolated serum and urinary antibodies to a single antigen. Immunology. 1966 Mar;10(3):249–258. [PMC free article] [PubMed] [Google Scholar]
  8. OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. Prog Allergy. 1958;5:1–78. [PubMed] [Google Scholar]
  9. PRAGER M. D., BEARDEN J. BLOOD GROUP ANTIBODIES IN HUMAN URINE. Transfusion. 1965 May-Jun;5:240–244. doi: 10.1111/j.1537-2995.1965.tb01164.x. [DOI] [PubMed] [Google Scholar]
  10. ROWE D. S., FAHEY J. L. A NEW CLASS OF HUMAN IMMUNOGLOBULINS. I. A UNIQUE MYELOMA PROTEIN. J Exp Med. 1965 Jan 1;121:171–184. doi: 10.1084/jem.121.1.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. ROWE D. S., FAHEY J. L. A NEW CLASS OF HUMAN IMMUNOGLOBULINS. II. NORMAL SERUM IGD. J Exp Med. 1965 Jan 1;121:185–199. doi: 10.1084/jem.121.1.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. STEVENSON G. T. Further studies of the gamma-related proteins of normal urine. J Clin Invest. 1962 May;41:1190–1198. doi: 10.1172/JCI104572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. TURNER M. W., ROWE D. S. CHARACTERIZATION OF HUMAN ANTIBODIES TO SALMONELLA TYPHI BY GEL-FILTRATION AND ANTIGENIC ANALYSIS. Immunology. 1964 Nov;7:639–656. [PMC free article] [PubMed] [Google Scholar]
  14. Turner M. W., Rowe D. S. A naturally occurring fragment related to the heavy chains of immunoglobulin G in normal human urine. Nature. 1966 Apr 9;210(5032):130–132. doi: 10.1038/210130a0. [DOI] [PubMed] [Google Scholar]

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