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. 1968 Jan;14(1):7–20.

A kinetic study of antibody producing cells in the spleen of mice immunized intravenously with sheep erythrocytes

G Biozzi, C Stiffel, D Mouton, Y Bouthillier, C Decreusefond
PMCID: PMC1409259  PMID: 5635755

Abstract

The number of antibody producing cells, i.e. rosette forming cells (RFC) has been studied in the spleen of mice injected intravenously with a full immunizing dose of sheep RBC.

The spleen of a non-immunized mouse contains a background of about 70,000 RFC (normal RFC), which do not appear to be the `target cells' for antigens of sheep RBC. Our findings suggest that the spleen of a mouse contains about 4000 `target cells' which initiate the immune response to sheep RBC.

In the primary response, the rise of RFC is exponential for about 96 hours with a doubling time of 13 hours involving seven to eight consecutive doubling periods. The peak value of RFC is 1·6 × 106 per spleen.

In the secondary response, the doubling time of RFC is 6–7 hours. The exponential rise lasts 72 hours and includes nine to eleven doubling periods leading to a peak of 3·5 × 106 RFC/spleen.

Adjuvant in the primary response leaves the doubling time of RFC unaltered but prolongs the exponential rise until the 120th hour leading to a peak of 6·3 × 106 RFC/spleen after ten doubling periods. The effects of priming and adjuvant are not fully additive.

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Selected References

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