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. 1968 Feb;14(2):181–196.

Quantitative assay of the lytic action of immune lymphoid cells of 51Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs

K T Brunner, J Mauel, J-C Cerottini, B Chapuis
PMCID: PMC1409286  PMID: 4966657

Abstract

The in vitro cytotoxic effect of spleen cells of mice immunized by tumour allografts was studied by measuring target cell inactivation as a function of release of radioactive label (51Cr) or loss of cloning efficiency. When sensitized lymphoid cells were incubated with target cells at a ratio of 100:1, up to 90 per cent of the incorporated label was released within 6–9 hours, while the number of clone-forming cells was reduced by up to 99 per cent in the same time period. Isoantiserum from the graft recipients, as well as its 19S and 7S fractions, protected target cells against the toxic effect of the spleen cells, but a lipoprotein antigen isolated from the tumour cells failed to inhibit the cytotoxic reaction. Target cell lysis as measured by specific release of 51Cr was partially inhibited by actinomycin-D and by cycloheximide at concentrations which effectively blocked DNA-dependent RNA and protein synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AL ASKARI S., DAVID J. R., LAWRENCE H. S., THOMAS L. IN VITRO STUDIES ON HOMOGRAFT SENSITIVITY. Nature. 1965 Feb 27;205:916–917. doi: 10.1038/205916a0. [DOI] [PubMed] [Google Scholar]
  2. AMOS D. B. Isoantigens of mouse red cells. Ann N Y Acad Sci. 1962 May 3;97:69–82. doi: 10.1111/j.1749-6632.1962.tb34623.x. [DOI] [PubMed] [Google Scholar]
  3. BITENSKY L. CYTOTOXIC ACTION OF ANTIBODIES. Br Med Bull. 1963 Sep;19:241–244. doi: 10.1093/oxfordjournals.bmb.a070064. [DOI] [PubMed] [Google Scholar]
  4. Bloom B. R., Bennett B. Mechanism of a reaction in vitro associated with delayed-type hypersensitivity. Science. 1966 Jul 1;153(3731):80–82. doi: 10.1126/science.153.3731.80. [DOI] [PubMed] [Google Scholar]
  5. Brunner K. T., Mauel J., Schindler R. In vitro studies of cell-bound immunity; cloning assay of the cytotoxic action of sensitized lymphoid cells on allogeneic target cells. Immunology. 1966 Nov;11(5):499–506. [PMC free article] [PubMed] [Google Scholar]
  6. Cerottini J. C., Brunner K. T. Localization of mouse isoantigens on the cell surface as revealed by immunofluorescence. Immunology. 1967 Oct;13(4):395–403. [PMC free article] [PubMed] [Google Scholar]
  7. DUNN T. B., POTTER M. A transplantable mast-cell neoplasm in the mouse. J Natl Cancer Inst. 1957 Apr;18(4):587–601. [PubMed] [Google Scholar]
  8. Davies D. A. Mouse histocompatibility isoantigens derived from normal and from tumour cells. Immunology. 1966 Aug;11(2):115–125. [PMC free article] [PubMed] [Google Scholar]
  9. FRIEDMAN H. INHIBITION OF ANTIBODY PLAQUE FORMATION BY SENSITIZED LYMPHOID CELLS: RAPID INDICATOR OF TRANSPLANTATION IMMUNITY. Science. 1964 Aug 7;145(3632):607–609. doi: 10.1126/science.145.3632.607. [DOI] [PubMed] [Google Scholar]
  10. MANSON L. A., FOSCHI G. V., DOUGHERTY T., PALM J. MICROSOMAL LIPOPROTEINS AS TRANSPLANTATION ANTIGENS. Ann N Y Acad Sci. 1964 Nov 30;120:251–260. doi: 10.1111/j.1749-6632.1964.tb34723.x. [DOI] [PubMed] [Google Scholar]
  11. MOELLER E. ANTAGONISTIC EFFECTS OF HUMORAL ISOANTIBODIES ON THE IN VITRO CYTOTOXICITY OF IMMUNE LYMPHOID CELLS. J Exp Med. 1965 Jul 1;122:11–23. doi: 10.1084/jem.122.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rosenau W., Moon H. D. Studies on the mechanism of the cytolytic effect of sensitized lymphocytes. J Immunol. 1966 Jan;96(1):80–84. [PubMed] [Google Scholar]
  13. VAINIO T., KOSKIMIES O., PERLMANN P., PERLMANN H., KLEIN G. IN VITRO CYTOTOXIC EFFECT OF LYMPHOID CELLS FROM MICE IMMUNIZED WITH ALLOGENEIC TISSUE. Nature. 1964 Oct 31;204:453–455. doi: 10.1038/204453a0. [DOI] [PubMed] [Google Scholar]
  14. WILSON D. B. QUANTITATIVE STUDIES ON THE BEHAVIOR OF SENSITIZED LYMPHOCYTES IN VITRO. II. INHIBITORY INFLUENCE OF THE IMMUNE SUPPRESSOR, IMURAN, ON THE DESTRUCTIVE REACTION OF SENSITIZED LYMPHOID CELLS AGAINST HOMOLOGOUS TARGET CELLS. J Exp Med. 1965 Jul 1;122:167–172. doi: 10.1084/jem.122.1.167. [DOI] [PMC free article] [PubMed] [Google Scholar]

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