Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1967 Aug;13(2):147–157.

A study of the `termination' of tolerance to BSA with DNP—BSA in rabbits: relative affinities of the antibodies for the immunizing and the paralysing antigens

W E Paul, G W Siskind, B Benacerraf
PMCID: PMC1409317  PMID: 4952101

Abstract

Rabbits rendered immunologically tolerant to BSA produced antibodies capable of precipitating BSA following immunization with DNP—BSA. Antigen binding studies using purified `anti-BSA' antibodies produced by tolerant animals indicated that these antibodies bound DNP—BSA with far greater avidity than BSA. It was concluded that a true termination of tolerance had not occurred. The implications of the results with regard to induction of antibody formation and of tolerance are discussed.

Full text

PDF
148

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENACERRAF B., LEVINE B. B. Immunological specificity of delayed and immediate hypersensitivity reactions. J Exp Med. 1962 May 1;115:1023–1036. doi: 10.1084/jem.115.5.1023. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CINADER B., DUBERT J. M. Acquired immune tolerance to human albumin and the response to subsequent injections of diazo human albumin. Br J Exp Pathol. 1955 Oct;36(5):515–529. [PMC free article] [PubMed] [Google Scholar]
  3. EISEN H. N., CARSTEN M. E., BELMAN S. Studies of hypersensitivity to low molecular weight substances. III. The 2,4-dinitrophenyl group as a determinant in the preciptin reaction. J Immunol. 1954 Nov;73(5):296–308. [PubMed] [Google Scholar]
  4. EISEN H. N. EQUILIBRIUM DIALYSIS FOR MEASUREMENT OF ANTIBODY-HAPTEN AFFINITIES. Methods Med Res. 1964;10:106–114. [PubMed] [Google Scholar]
  5. FARR R. S. A quantitative immunochemical measure of the primary interaction between I BSA and antibody. J Infect Dis. 1958 Nov-Dec;103(3):239–262. doi: 10.1093/infdis/103.3.239. [DOI] [PubMed] [Google Scholar]
  6. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HUMPHREY J. H. IMMUNOLOGICAL UNRESPONSIVENESS TO PROTEIN ANTIGENS IN RABBITS. I. THE DURATION OF UNRESPONSIVENESS FOLLOWING A SINGLE INJECTION AT BIRTH. Immunology. 1964 Jul;7:449–461. [PMC free article] [PubMed] [Google Scholar]
  8. Linscott W. D., Weigle W. O. Anti-bovine serum albumin specificity and binding affinity after termination of tolerance to bovine serum albumin. J Immunol. 1965 Sep;95(3):546–558. [PubMed] [Google Scholar]
  9. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  10. Nachtigel D., Eschel-Zussman R., Feldman M. Restoration of the specific immunological reactivity of tolerant rabbits by conjugated antigens. Immunology. 1965 Dec;9(6):543–551. [PMC free article] [PubMed] [Google Scholar]
  11. OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. II. Prog Allergy. 1962;6:30–154. doi: 10.1159/000313795. [DOI] [PubMed] [Google Scholar]
  12. SCHECHTER I., BAUMINGER S., SELA M., NACHTIGAL D., FELDMAN M. IMMUNE RESPONSE TO POLYPEPTIDYL PROTEINS IN RABBITS TOLERANT TO THE PROTEIN CARRIERS. Immunochemistry. 1964 Dec;1:249–265. doi: 10.1016/0019-2791(64)90026-6. [DOI] [PubMed] [Google Scholar]
  13. Siskind G. W., Paul W. E., Benacerraf B. Studies on the effect of the carrier molecule on antihapten antibody synthesis. I. Effect of carrier on the nature of the antibody synthesized. J Exp Med. 1966 Apr 1;123(4):673–688. doi: 10.1084/jem.123.4.673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Thorbecke G. J., Benacerraf B. Tolerance in adult rabbits by repeated non-immunogenic doses of bovine serum albumin. Immunology. 1967 Aug;13(2):141–145. [PMC free article] [PubMed] [Google Scholar]
  15. WEIGLE W. O. THE IMMUNE RESPONSE OF BSA TOLERANT RABBITS TO INJECTIONS OF BSA FOLLOWING THE TERMINATION OF THE TOLERANT STATE. J Immunol. 1964 May;92:791–797. [PubMed] [Google Scholar]
  16. WEIGLE W. O. Termination of acquired immunological tolerance to protein antigens following immunization with altered protein antigens. J Exp Med. 1962 Dec 1;116:913–928. doi: 10.1084/jem.116.6.913. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. WEIGLE W. O. The immune response of rabbits tolerant to bovine serum albumin to the injection of other heterologous serum albumins. J Exp Med. 1961 Jul 1;114:111–125. doi: 10.1084/jem.114.1.111. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES