Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1968 May;14(5):725–734.

Immunoglobulin classes in antibody responses in mice

I. Analysis by biological properties

N L Warner, N M Vaz, Z Ovary
PMCID: PMC1409398  PMID: 4172979

Abstract

The formation of different immunoglobulin classes of mouse antibodies to dinitrophenol (DNP) has been qualitatively determined by biological assays which discriminate between some immunoglobulin classes. Antibody responses to two other antigens were compared in five mouse strains. Mice of five different strains immunized with DNP—haemocyanin (DNP—Hcy) in complete Freund's adjuvant made both γG1 and γG2a antibodies. The amounts of antibody are less in DBA and C57 mice and at 80 days after primary immunization a 2-mercaptoethanol sensitive (2MES) antibody is still present in these strains (but not in A, AKR and C3H). When DNP—Hcy is given in Al(OH)3, very little γG2a is produced in any strain, although γG1 antibodies are produced as in mice given DNP—Hcy in complete Freund's adjuvant. Possible interpretations of these observations are discussed.

Full text

PDF
734

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENACERRAF B., OVARY Z., BLOCH K. J., FRANKLIN E. C. Properties of guinea pig 7S antibodies. I. Electrophoretic separation of two types of guinea pig 7S antibodies. J Exp Med. 1963 Jun 1;117:937–949. doi: 10.1084/jem.117.6.937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BLOCH K. J., KOURILSKY F. M., OVARY Z., BENACERRAF B. Properties of guinea pig 7S antibodies. III. Identification of antibodies involved in complement fixation and hemolysis. J Exp Med. 1963 Jun 1;117:965–981. doi: 10.1084/jem.117.6.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BOYDEN S. V. The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. J Exp Med. 1951 Feb;93(2):107–120. doi: 10.1084/jem.93.2.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BULLOCK W. E., KANTOR F. S. HEMAGGLUTINATION REACTIONS OF HUMAN ERYTHROCYTES CONJUGATED COVALENTLY WITH DINITROPHENYL GROUPS. J Immunol. 1965 Mar;94:317–322. [PubMed] [Google Scholar]
  5. Barth W. F., Fahey J. L. Heterologous and homologous skin-sensitizing activity of mouse 7S gamma 1- and 7S gamma 2-globulins. Nature. 1965 May 15;206(985):730–731. doi: 10.1038/206730a0. [DOI] [PubMed] [Google Scholar]
  6. Barth W. F., McLaughlin C. L., Fahey J. L. The immunoglobulins of mice. VI. Response to immunization. J Immunol. 1965 Nov;95(5):781–790. [PubMed] [Google Scholar]
  7. Borsos T., Rapp H. J. Complement fixation on cell surfaces by 19S and 7S antibodies. Science. 1965 Oct 22;150(3695):505–506. doi: 10.1126/science.150.3695.505. [DOI] [PubMed] [Google Scholar]
  8. Claflin A. J., Smithies O., Meyer R. K. Antibody responses in bursa-deficient chickens. J Immunol. 1966 Nov;97(5):693–699. [PubMed] [Google Scholar]
  9. Coe J. E. 7Sgamma-1 and 7Sgamma-2 antibody response in the mouse. I. Influence of strain, antigen and adjuvant. J Immunol. 1966 Apr;96(4):744–753. [PubMed] [Google Scholar]
  10. FAHEY J. L., WUNDERLICH J., MISHELL R. THE IMMUNOGLOBULINS OF MICE. I. FOUR MAJOR CLASSES OF IMMUNOGLOBULINS: 7S GAMMA-2-, 7S GAMMA-1-, GAMMA-1A (BETA-2A)-, AND 18S GAMMA-1M-GLOBULINS. J Exp Med. 1964 Aug 1;120:223–242. doi: 10.1084/jem.120.2.223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. FAHEY J. L., WUNDERLICH J., MISHELL R. THE IMMUNOGLOBULINS OF MICE. II. TWO SUBCLASSES OF MOUSE 7S GAMMA-2-GLOBULINS: GAMMA-2A- AND GAMMA-2B-GLOBULINS. J Exp Med. 1964 Aug 1;120:243–251. doi: 10.1084/jem.120.2.243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. FINK M. A., QUINN V. A. Antibody production in inbred strains of mice. J Immunol. 1953 Jan;70(1):61–67. [PubMed] [Google Scholar]
  13. Fudenberg H. H. The immune globulins. Annu Rev Microbiol. 1965;19:301–338. doi: 10.1146/annurev.mi.19.100165.001505. [DOI] [PubMed] [Google Scholar]
  14. HERZENBERG L. A., WARNER N. L., HERZENBERG L. A. IMMUNOGLOBULIN ISOANTIGENS (ALLOTYPES) IN THE MOUSE. I. GENETICS AND CROSS-REACTIONS OF THE 7S GAMMA-2A-ISOANTIGENS CONTROLLED BY ALLELES AT THE IG-1 LOCUS. J Exp Med. 1965 Mar 1;121:415–438. doi: 10.1084/jem.121.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LEVINE B. B., OJEDA A., BENACERRAF B. STUDIES ON ARTIFICIAL ANTIGENS. III. THE GENETIC CONTROL OF THE IMMUNE RESPONSE TO HAPTEN-POLY-L-LYSINE CONJUGATES IN GUINEA PIGS. J Exp Med. 1963 Dec 1;118:953–957. doi: 10.1084/jem.118.6.953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lennox E. S. The genetics of the immune response. Proc R Soc Lond B Biol Sci. 1966 Nov 22;166(1003):222–231. doi: 10.1098/rspb.1966.0095. [DOI] [PubMed] [Google Scholar]
  17. McDevitt H. O., Sela M. Genetic control of the antibody response. I. Demonstration of determinant-specific differences in response to synthetic polypeptide antigens in two strains of inbred mice. J Exp Med. 1965 Sep 1;122(3):517–531. doi: 10.1084/jem.122.3.517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. NOSSAL G. J., SZENBERG A., ADA G. L., AUSTIN C. M. SINGLE CELL STUDIES ON 19S ANTIBODY PRODUCTION. J Exp Med. 1964 Mar 1;119:485–502. doi: 10.1084/jem.119.3.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. NUSSENZWEIG R. S., MERRYMAN C., BENACERRAF B. ELECTROPHORETIC SEPARATION AND PROPERTIES OF MOUSE ANTIHAPTEN ANTIBODIES INVOLVED IN PASSIVE CUTANEOUS ANAPHYLAXIS AND PASSIVE HEMOLYSIS. J Exp Med. 1964 Aug 1;120:315–328. doi: 10.1084/jem.120.2.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. OVARY Z., BARTH W. F., FAHEY J. L. THE IMMUNOGLOBULINS OF MICE. 3. SKIN SENSITIZING ACTIVITY OF MOUSE IMMUNOGLOBULINS. J Immunol. 1965 Mar;94:410–415. [PubMed] [Google Scholar]
  21. OVARY Z., BENACERRAF B., BLOCH K. J. Properties of guinea pig 7S antibodies. II. Identification of antibodies involved in passive cutaneous and systemic anaphylaxis. J Exp Med. 1963 Jun 1;117:951–964. doi: 10.1084/jem.117.6.951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. OVARY Z., BENACERRAF B. IMMUNOLOGICAL SPECIFICITY OF THE SECONDARY RESPONSE WITH DINITROPHENYLATED PROTEINS. Proc Soc Exp Biol Med. 1963 Oct;114:72–76. doi: 10.3181/00379727-114-28589. [DOI] [PubMed] [Google Scholar]
  23. ROTHBERG R., TALMAGE D. W. Circulating antibody and anaphylaxis in mice. J Immunol. 1961 Mar;86:302–306. [PubMed] [Google Scholar]
  24. ROWLEY D., JENKIN C. R. Antigenic cross-reaction between host and parasite as a possible cause of pathogenicity. Nature. 1962 Jan 13;193:151–154. doi: 10.1038/193151a0. [DOI] [PubMed] [Google Scholar]
  25. Rapaport F. T., Chase R. M., Jr The bacterial induction of homograft sensitivity. II. Effects of sensitization with staphylococci and other microoorganisms. J Exp Med. 1965 Oct 1;122(4):733–744. doi: 10.1084/jem.122.4.733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. SZENBERG A., LIND P., CLARKE K. IGG AND IGM ANTIBODIES IN FOWL SERUM. Aust J Exp Biol Med Sci. 1965 Jul;43:451–454. doi: 10.1038/icb.1965.79. [DOI] [PubMed] [Google Scholar]
  27. WHITE R. G., JENKINS G. C., WILKINSON P. C. The production of skin-sensitizing antibody in the guinea-pig. Int Arch Allergy Appl Immunol. 1963;22:156–165. doi: 10.1159/000229362. [DOI] [PubMed] [Google Scholar]
  28. Warner N. L., Herzenberg L. A., Goldstein G. Immunoglobulin isoantigens (allotypes) in the mouse. II. Allotypic analysis of three gammaG2-myeloma proteins from (NZB x BALB/c)F1 hybrids and of normal gammaG2-globulins. J Exp Med. 1966 Apr 1;123(4):707–721. doi: 10.1084/jem.123.4.707. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES