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. 1968 May;14(5):621–635.

Graft versus host reaction in tissue culture

I. Lysis of monolayers of embryo mouse cells from strains differing in the H-2 histocompatability locus by rat lymphocytes sensitized in vitro

H Ginsburg
PMCID: PMC1409407  PMID: 5656875

Abstract

Rat lymphocytes cultured on mouse embryo cell monolayers produced large pyroninophilic cells (LPC) which lysed the mouse cells. The LPC that developed on monolayers of any particular strain of mouse (originator monolayers) were tested, by transfer, for their ability to lyse monolayers of other mouse strains. The distribution of lysis among the various strains of mouse revealed a definite pattern of specificity. Analysis of the H-2 allelic complement of the mouse strains tested suggests that the lymphocytes were sensitized upon exposure to the mouse embryo monolayers against one or more of the antigens determined by the H-2 locus. The presence or absence of one or all of the antigens in other strains determined whether monolayers of these strains were lysed completely, partially, or not at all. It was concluded that the cultures obtained are an in vitro reflection of the graft versus host immune reaction. It was produced in the tissue culture as a primary response by normal lymphocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. GESNER B. M., GOWANS J. L. The fate of lethally irradiated mice given isologous and heterologous thoracic duct lymphocytes. Br J Exp Pathol. 1962 Aug;43:431–440. [PMC free article] [PubMed] [Google Scholar]
  2. GOWANS J. L. The fate of parental strain small lymphocytes in F1 hybrid rats. Ann N Y Acad Sci. 1962 Oct 24;99:432–455. doi: 10.1111/j.1749-6632.1962.tb45326.x. [DOI] [PubMed] [Google Scholar]
  3. Ginsburg H., Lagunoff D. The in vitro differentiation of mast cells. Cultures of cells from immunized mouse lymph nodes and thoracic duct lymph on fibroblast monolayers. J Cell Biol. 1967 Dec;35(3):685–697. doi: 10.1083/jcb.35.3.685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ginsburg H., Sachs L. Destruction of mouse and rat embryo cells in tissue culture by lymph node cells from unsensitized rats. J Cell Physiol. 1965 Oct;66(2):199–219. doi: 10.1002/jcp.1030660207. [DOI] [PubMed] [Google Scholar]
  5. SACHS L., FELDMAN M. Cytotoxic antibodies in the homograft reaction. J Natl Cancer Inst. 1958 Sep;21(3):563–578. [PubMed] [Google Scholar]
  6. SNELL G. D., HOECKER G., AMOS D. B., STIMPFLING J. H. A REVISED NOMENCLATURE FOR THE HISTOCOMPATIBILITY-2 LOCUS OF THE MOUSE. Transplantation. 1964 Nov;2:777–784. doi: 10.1097/00007890-196411000-00010. [DOI] [PubMed] [Google Scholar]
  7. WAYMOUTH C. Rapid proliferation of sublines of NCTC clone 929 (strain L) mouse cells in a simple chemically defined medium (MB 752/1). J Natl Cancer Inst. 1959 May;22(5):1003–1017. doi: 10.1093/jnci/22.5.1003. [DOI] [PubMed] [Google Scholar]

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