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. 1968 May;14(5):649–655.

Formation of C′6 by rabbit liver tissue in vitro

Ursula Rother, G Jeanette Thorbecke, Vera J Stecher-Levin, J Hurlimann, K Rother
PMCID: PMC1409416  PMID: 4173565

Abstract

Various rabbit tissues such as liver, spleen, peritoneal macrophages and kidney, were cultured in vitro at 37° for periods of up to 72 hours. The combined content of C′6 in tissue and culture medium was determined at the end of the incubation period. Production of C′6 activity was found in liver cultures and possibly in spleen and macrophage cultures. The rate of inactivation of serum C′6 at 37° in culture medium was also determined.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Colten H. R., Borsos T., Rapp H. J. In vitro synthesis of the first component of complement by guinea pig small intestine. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1158–1163. doi: 10.1073/pnas.56.4.1158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hurlimann J., Thorbecke G. J., Hochwald G. M. The liver as the site of C-reactive protein formation. J Exp Med. 1966 Feb 1;123(2):365–378. doi: 10.1084/jem.123.2.365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. LINSCOTT W. D., COCHRANE C. G. GUINEA PIG BETA-1C-GLOBULIN: ITS RELATIONSHIP TO THE THIRD COMPONENT OF COMPLEMENT AND ITS ALTERATION FOLLOWING INTERACTION WITH IMMUNE COMPLEXES. J Immunol. 1964 Dec;93:972–984. [PubMed] [Google Scholar]
  4. MARDINEY M. R., Jr, MUELLER-EBERHARD H. J. MOUSE BETA-1C-GLOBULIN: PRODUCTION OF ANTISERUM AND CHARACTERIZATION IN THE COMPLEMENT REACTION. J Immunol. 1965 Jun;94:877–882. [PubMed] [Google Scholar]
  5. MORSE J. H., CHRISTIAN C. L. IMMUNOLOGICAL STUDIES OF THE 11S PROTEIN COMPONENT OF THE HUMAN COMPLEMENT SYSTEM. J Exp Med. 1964 Feb 1;119:195–209. doi: 10.1084/jem.119.2.195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. MUELLER-EBERHARD H. J., BIRO C. E. ISOLATION AND DESCRIPTION OF THE FOURTH COMPONENT OF HUMAN COMPLEMENT. J Exp Med. 1963 Sep 1;118:447–466. doi: 10.1084/jem.118.3.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MULLER-EBERHARD H. J., KUNKEL H. G. Isolation of a thermolabile serum protein which precipitates gamma-globulin aggregates and participates in immune hemolysis. Proc Soc Exp Biol Med. 1961 Feb;106:291–295. doi: 10.3181/00379727-106-26313. [DOI] [PubMed] [Google Scholar]
  8. MULLER-EBERHARD H. J., NILSSON U., ARONSSON T. Isolation and characterization of two beta1-glycoproteins of human serum. J Exp Med. 1960 Feb 1;111:201–215. doi: 10.1084/jem.111.2.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. NISHIOKA K., LINSCOTT W. D. COMPONENTS OF GUINEA PIG COMPLEMENT. I. SEPARATION OF A SERUM FRACTION ESSENTIAL FOR IMMUNE HEMOLYSIS AND IMMUNE ADHERENCE. J Exp Med. 1963 Nov 1;118:767–793. doi: 10.1084/jem.118.5.767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rother K., Rother U., Müller-Eberhard H. J., Nilsson J. R. Deficiency of the sixth component of complement in rabbits with an inherited complement defect. J Exp Med. 1966 Oct 1;124(4):773–785. doi: 10.1084/jem.124.4.773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SIBOO R., VAS S. I. STUDIES ON IN VITRO ANTIBODY PRODUCTION. 3. PRODUCTION OF COMPLEMENT. Can J Microbiol. 1965 Jun;11:415–425. doi: 10.1139/m65-055. [DOI] [PubMed] [Google Scholar]
  12. Stecher V. J., Morse J. H., Thorbecke G. J. Sites of production of primate serum proteins associated with complement system. Proc Soc Exp Biol Med. 1967 Feb;124(2):433–438. doi: 10.3181/00379727-124-31758. [DOI] [PubMed] [Google Scholar]
  13. Stecher V. J., Thorbecke G. J. Beta-1C and immune globulin formation in vitro by tissues from germ-free and conventional rodents of various ages. Immunology. 1967 Apr;12(4):475–487. [PMC free article] [PubMed] [Google Scholar]
  14. Stecher V. J., Thorbecke G. J. Sites of synthesis of serum proteins. OI. Serum proteins produced by macrophages in vitro. J Immunol. 1967 Oct;99(4):643–652. [PubMed] [Google Scholar]
  15. WILLIAMS C. A., Jr, ASOFSKY R., THORBECKE G. J. PLASMA PROTEIN FORMATION IN VITRO BY TISSUES FROM MICE INFECTED WITH STAPHYLOCOCCI. J Exp Med. 1963 Aug 1;118:315–326. doi: 10.1084/jem.118.2.315. [DOI] [PMC free article] [PubMed] [Google Scholar]

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