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. 1968 Nov;15(5):697–705.

Notes on the preparatio and assay of anti-lymphocyte serum for use in mice

Somarie V Jooste, E M Lance, R H Levey, P B Medawar, M Ruszkiewicz, Ruth Sharman, R N Taub
PMCID: PMC1409531  PMID: 4880356

Abstract

The `activity' of rabbit anti-mouse ALS was measured in terms of its power to prolong the life of A-strain homografts on CBA mice. Antisera raised by lymphocytes from mice of other strains were active in CBA mice, and conversely antisera raised by CBA lymphocytes were active in mice of other strains. All active sera were cytotoxic to mouse lymphocytes in the presence of mouse complement, but not all cytotoxic sera were active. ALS raised in species (chicken, duck) of which the sera do not bind with mouse complement were inactive in mice. Neither cytotoxicity nor the power to circumvent graft-versus-host reactions give adequate measures of the activity of impure antisera.

Good antiserum (`two-pulse' ALS) for use in mice could be raised in New Zealand White rabbits by the intravenous injection of 109 mouse thymocytes on two occasions 14 days apart, followed by bleeding out 7 days after the second injection. Further injections of antigen increased the titre of undesirable contaminants in ALS (e.g. red cell agglutinins) but usually did not increase (and sometimes lowered) activity. ALS raised with adjuvants, though powerful, was highly toxic and required extensive absorption.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASHWOOD-SMITH M. J. LOW TEMPERATURE PRESERVATION OF MOUSE LYMPHOCYTES WITH DIMETHYL SULFOXIDE. Blood. 1964 Apr;23:494–501. [PubMed] [Google Scholar]
  2. Boak J. L., Fox M., Wilson R. E. Activity of lymphoid tissues from antilymphocyte-serum-treated mice. Lancet. 1967 Apr 8;1(7493):750–752. doi: 10.1016/s0140-6736(67)91366-9. [DOI] [PubMed] [Google Scholar]
  3. GOODMAN H. S. A general method for the quantitation of immune cyotysis. Nature. 1961 Apr 15;190:269–270. doi: 10.1038/190269a0. [DOI] [PubMed] [Google Scholar]
  4. GRAY J. G., MONACO A. P., RUSSELL P. S. HETEROLOGOUS MOUSE ANTI-LYMPHOCYTE SERUM TO PROLONG SKIN HOMOGRAFTS. Surg Forum. 1964;15:142–144. [PubMed] [Google Scholar]
  5. James K. Anti-lymphocytic antibody--a review. Clin Exp Immunol. 1967 Nov;2(6):615–631. [PMC free article] [PubMed] [Google Scholar]
  6. James K., Medawar P. B. Characterization of antilymphocytic antibody. Nature. 1967 Jun 3;214(5092):1052–1053. doi: 10.1038/2141052b0. [DOI] [PubMed] [Google Scholar]
  7. Jeejeebhoy H. F. Immunological studies on the rat thymectomized in adult life. Immunology. 1965 Nov;9(5):417–425. [PMC free article] [PubMed] [Google Scholar]
  8. KALISS N. Immunological enhancement of tumor homografts in mice: a review. Cancer Res. 1958 Oct;18(9):992–1003. [PubMed] [Google Scholar]
  9. Lance E. M., Batchelor J. R. Selective suppression of cellular immunity by antilymphocyte serum. Transplantation. 1968 May;6(3):490–491. doi: 10.1097/00007890-196805000-00027. [DOI] [PubMed] [Google Scholar]
  10. Lance E. M., Dresser D. W. Antigenicity in mice of antilymphocyte gamma-globulin. Nature. 1967 Jul 29;215(5100):488–490. doi: 10.1038/215488a0. [DOI] [PubMed] [Google Scholar]
  11. Levey R. H., Medawar P. B. Further experiments on the action of antilymphocytic antiserum. Proc Natl Acad Sci U S A. 1967 Aug;58(2):470–477. doi: 10.1073/pnas.58.2.470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Levey R. H., Medawar P. B. Nature and mode of action of antilymphocytic antiserum. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1130–1137. doi: 10.1073/pnas.56.4.1130. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mosedale B., Felstead K. J., Parke J. A. The effect of anti-lymphocyte serum on the response of human and mouse lymphocytes to PHA. Nature. 1968 Jun 8;218(5145):983–984. doi: 10.1038/218983a0. [DOI] [PubMed] [Google Scholar]
  14. Nagaya H., Sieker H. O. Lymphopenic effect of anti-lymphocyte serum. Proc Soc Exp Biol Med. 1966 Mar;121(3):722–725. doi: 10.3181/00379727-121-30870. [DOI] [PubMed] [Google Scholar]
  15. SANDERSON A. R. CYTOTOXIC REACTIONS OF MOUSE ISO-ANTISERA: PRELIMINARY CONSIDERATIONS. Br J Exp Pathol. 1964 Aug;45:398–408. [PMC free article] [PubMed] [Google Scholar]
  16. Taub R. N., Lance E. M. Effects of heterologous anti-lymphocyte serum on the distribution of 51-Cr-labelled lymph node cells in mice. Immunology. 1968 Nov;15(5):633–642. [PMC free article] [PubMed] [Google Scholar]
  17. WIGZELL H. QUANTITATIVE TITRATIONS OF MOUSE H-2 ANTIBODIES USING CR-51-LABELLED TARGET CELLS. Transplantation. 1965 May;3:423–431. doi: 10.1097/00007890-196505000-00011. [DOI] [PubMed] [Google Scholar]
  18. WOODRUFF M. F., ANDERSON N. A. EFFECT OF LYMPHOCYTE DEPLETION BY THORACIC DUCT FISTULA AND ADMINISTRATION OF ANTILYMPHOCYTIC SERUM ON THE SURVIVAL OF SKIN HOMOGRAFTS IN RATS. Nature. 1963 Nov 16;200:702–702. doi: 10.1038/200702a0. [DOI] [PubMed] [Google Scholar]

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