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. 1969 Mar;16(3):381–386.

The relationship between lymphocyte transformation and immune response

I. Ratio of transforming cells to antibody forming cells

I Gery, D Benezra, A M Davies
PMCID: PMC1409625  PMID: 5770385

Abstract

A comparison was made between the number of cells forming or carrying antibody and those stimulated to transform in vitro by antigen, by using blood lymphocytes from rabbits immunized against sheep erythrocytes.

In blood samples collected 7–77 days after a single immunization, the number of transformed cells was always markedly higher than that of cells forming plaques or rosettes.

It is suggested that unsensitized cells are induced to transform by a stimulus transferred from other reacting cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker P. J., Landy M. Brevity of the inductive phase in the immune response of mice to capsular polysaccharide antigens. J Immunol. 1967 Oct;99(4):687–694. [PubMed] [Google Scholar]
  2. Benezra D., Gery I., Davies A. M. Transformation of rabbit lymphocytes by specific antigens. Proc Soc Exp Biol Med. 1967 Aug-Sep;125(4):1305–1308. doi: 10.3181/00379727-125-32342. [DOI] [PubMed] [Google Scholar]
  3. Berman L. Lymphocytes and macrophages in vitro. Their activties in relation to functions of small lymphocytes. Lab Invest. 1966 Jun;15(6):1084–1099. [PubMed] [Google Scholar]
  4. Bloom B. R., Bennett B. Migration inhibitory factor associated with delayed-type hypersensitivity. Fed Proc. 1968 Jan-Feb;27(1):13–15. [PubMed] [Google Scholar]
  5. Caron G. A. The effect of antigens in combination on lymphocyte transformation in vitro. Int Arch Allergy Appl Immunol. 1967;31(6):521–528. doi: 10.1159/000229897. [DOI] [PubMed] [Google Scholar]
  6. Chalmers D. G., Cooper E. H., Evans C., Topping N. E. Quantitation of the response of lymphocytes in culture to specific and non-specific stimulation. Int Arch Allergy Appl Immunol. 1967;32(2):117–130. doi: 10.1159/000229922. [DOI] [PubMed] [Google Scholar]
  7. Dutton R. W. In vitro studies of immunological responses of lymphoid cells. Adv Immunol. 1967;6:253–336. doi: 10.1016/s0065-2776(08)60524-6. [DOI] [PubMed] [Google Scholar]
  8. Gowans J. L., Uhr J. W. The carriage of immunological memory by small lymphocytes in the rat. J Exp Med. 1966 Nov 1;124(5):1017–1030. doi: 10.1084/jem.124.5.1017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Green I., Vassalli P., Nussenzweig V., Benacerraf B. Specificity of the antibodies produced by single cells following immunization with antigens bearing two types of antigenic determinants. J Exp Med. 1967 Mar 1;125(3):511–526. doi: 10.1084/jem.125.3.511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Johnson L. I., Chan P. C., Lobue J., Monette F. C., Gordon A. S. Cell cycle analysis of rat lymphocytes cultured with phytohemagglutinin in diffusion chambers. Exp Cell Res. 1967 Aug;47(1):201–208. doi: 10.1016/0014-4827(67)90223-6. [DOI] [PubMed] [Google Scholar]
  11. MCFARLAND W., HEILMAN D. H. LYMPHOCYTE FOOT APPENDAGE: ITS ROLE IN LYMPHOCYTE FUNCTION AND IN IMMUNOLOGICAL REACTIONS. Nature. 1965 Feb 27;205:887–888. doi: 10.1038/205887a0. [DOI] [PubMed] [Google Scholar]
  12. Mayron L. W., Baram P. The effect of antigen on the rate of guanosine-8-C14 incorporation by lymphocytes. J Immunol. 1967 Jun;98(6):1274–1285. [PubMed] [Google Scholar]
  13. Moorhead J. F., Connolly J. J., McFarland W. Factors affecting the reactivity of human lymphocytes in vitro. I. Cell number, duration of culture and surface area. J Immunol. 1967 Aug;99(2):413–419. [PubMed] [Google Scholar]
  14. Sasaki M. S., Norman A. Proliferation of human lymphocytes in culture. Nature. 1966 May 28;210(5039):913–914. doi: 10.1038/210913a0. [DOI] [PubMed] [Google Scholar]
  15. Sell S. Studies on rabbit lymphocytes in vitro. VI. The induction of blast transformation with sheep antisera to rabbit IgA and IgM. J Exp Med. 1967 Mar 1;125(3):393–400. doi: 10.1084/jem.125.3.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Vischer T. L., Stastny P. Time of appearance and distribution of cells capable of secondary immune response following primary immunization. Immunology. 1967 Jun;12(6):675–687. [PMC free article] [PubMed] [Google Scholar]
  17. Wortis H. H., Taylor R. B., Dresser D. W. Antibody production studied by means of the LHG assay. I. The splenic response of CBA mice to sheep erythrocytes. Immunology. 1966 Dec;11(6):603–616. [PMC free article] [PubMed] [Google Scholar]
  18. Zaalberg O. B., van der Meul V. A., van Twisk J. M. Antibody production by single spleen cells: a comparative study of the cluster and agar-plaque formation. Nature. 1966 Apr 30;210(5035):544–545. doi: 10.1038/210544a0. [DOI] [PubMed] [Google Scholar]
  19. Zaalberg O. B., van der Meul V. A., van Twisk M. J. Antibody production by isolated spleen cells: a study of the cluster and the plaque techniques. J Immunol. 1968 Feb;100(2):451–458. [PubMed] [Google Scholar]

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