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. 1969 Apr;16(4):447–461.

Cytolysis of mouse lymph node cells by alloantibody: a comparison of guinea-pig and rabbit complements

G Haughton, M Patricia McGehee
PMCID: PMC1409635  PMID: 4181540

Abstract

Rabbit serum obtained from selected animals, and absorbed with mouse liver and spleen, was used as a source of complement in the 51Cr monitored cytolysis of mouse lymph node cells by H-2 alloantibody. Antibody was more efficiently cytolytic in the presence of rabbit complement (RC′) than in the presence of guinea-pig complement (GPC′). Addition of rabbit or goat anti-mouse globulin to GPC′ caused a modest increase in the cytolytic efficiency of the alloantibody. Addition of antiglobulin to RC′ reduced the cytolytic efficiency of alloantibody. The slope of the dilution curve of H-2 antibody was less steep in the presence of RC′ than in the presence of GPC′. The apparent order of reaction with respect to antibody was lower with RC′ than with GPC′. Mouse heteroantibody against sheep erythrocytes was slightly more haemolytically efficient in the presence of GPC′ than in the presence of RC′. The dilution curves of heteroantibody in the presence of RC′ or GPC′ had the same slope, and the apparent orders of reaction with respect to antibody were similar. A greater concentration of GPC was required to complement the alloantibody than the heteroantibody whereas a similar concentration of RC′ sufficed in the two systems. Possible interpretations of these results are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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