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. 1969 Jun;16(6):737–747.

The antibody activities of 19S and 7S fractions from rabbit antisera to Bordetella pertussis

Jean M Dolby, D E Dolby
PMCID: PMC1409663  PMID: 4306784

Abstract

Bordetella pertussis antisera were fractionated on Sephadex G-200 to give 19S and 7S globulins, the latter refractionated on DEAE-cellulose, and the antibody properties of the two purified materials compared.

The minimal weights of 19S and 7S globulins required to neutralize haemagglutinin and to protect mice against an intranasal challenge causing lung infection were similar. In agglutination 7S globulin was 1.5–100 times more effective than 19S, the ratio depending on strain and serum, but there was no apparent correlation with specific agglutinogens.

7S globulin was at least 100 times more effective than 19S both in the complement-dependent bactericidal reaction in vitro and in the protection of mice against an intracerebral challenge. It is suggested that the two reactions measure the same antibody.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSEN E. K. Serological studies on H. pertussis H. parapertussis and H. bronchisepticus. Acta Pathol Microbiol Scand. 1953;33(2):202–224. doi: 10.1111/j.1699-0463.1953.tb01512.x. [DOI] [PubMed] [Google Scholar]
  2. Bladen H. A., Evans R. T., Mergenhagen S. E. Lesions in Escherichia coli membranes after action of antibody and complement. J Bacteriol. 1966 Jun;91(6):2377–2381. doi: 10.1128/jb.91.6.2377-2381.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Borsos T., Rapp H. J. Complement fixation on cell surfaces by 19S and 7S antibodies. Science. 1965 Oct 22;150(3695):505–506. doi: 10.1126/science.150.3695.505. [DOI] [PubMed] [Google Scholar]
  4. Daguillard F., Edsall G. The agglutinating and bactericidal activity of IgM and IgG antibodies to the 9 and 12 factors of Salmonella typhi O 901. J Immunol. 1968 May;100(5):1112–1120. [PubMed] [Google Scholar]
  5. Dolby J. M. The antibacterial effect of Bordetella pertussis antisera. Immunology. 1965 May;8(5):484–498. [PMC free article] [PubMed] [Google Scholar]
  6. Dolby J. M., Vincent W. A. Characterization of the antibodies responsible for the 'bactericidal activity patterns' of antisera to Bordetella pertussis. Immunology. 1965 May;8(5):499–510. [PMC free article] [PubMed] [Google Scholar]
  7. ELDERING G., HORNBECK C., BAKER J. Serological study of Bordetella pertussis and related species. J Bacteriol. 1957 Aug;74(2):133–136. doi: 10.1128/jb.74.2.133-136.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Frommel D., Grob P. J., Masouredis S. P., Isliker H. C. Studies on the mechanism of immunoglobulin binding to red cells. Immunology. 1967 Nov;13(5):501–508. [PMC free article] [PubMed] [Google Scholar]
  9. HEREMANS J. F., VAERMAN J. P., VAERMAN C. STUDIES ON THE IMMUNE GLOBULINS OF HUMAN SERUM. II. A STUDY OF THE DISTRIBUTION OF ANTI-BRUCELLA AND ANTI-DIPHTHERIA ANTIBODY ACTIVITIES AMONG GAMMA-SS, GAMMA-IM AND GAMMA-1A-GLOBULIN FRACTIONS. J Immunol. 1963 Jul;91:11–17. [PubMed] [Google Scholar]
  10. HIRSCHFELD J. Individual precipitation patterns of normal rabbit sera. A preliminary report. Acta Pathol Microbiol Scand. 1959;46:229–238. doi: 10.1111/j.1699-0463.1959.tb00334.x. [DOI] [PubMed] [Google Scholar]
  11. HOLT L. B., SPASOJEVIC V., DOLBY J. M., STANDFAST A. F. Immunity in mice to an intracerebral challenge of Bordetella pertussis. J Hyg (Lond) 1961 Sep;59:373–378. doi: 10.1017/s0022172400039036. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Herzberg M., Kenny K. V., Robbins J. B. Nonabsorbable rabbit anti-Salmonella typhimurium antibody as detected by the complement-mediated bactericidal reaction. J Bacteriol. 1966 Apr;91(4):1548–1555. doi: 10.1128/jb.91.4.1548-1555.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Humphrey J. H. Haemolytic efficiency of rabbit IgG anti-Forssman antibody and its augmentation by anti-rabbit IgG. Nature. 1967 Dec 30;216(5122):1295–1296. doi: 10.1038/2161295a0. [DOI] [PubMed] [Google Scholar]
  14. Landy M., Sanderson R. P., Jackson A. L. Humoral and cellular aspects of the immune response to the somatic antigen of Salmonella enteritidis. J Exp Med. 1965 Sep 1;122(3):483–504. doi: 10.1084/jem.122.3.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pike R. M. Antibody heterogeneity and serological reactions. Bacteriol Rev. 1967 Jun;31(2):157–174. doi: 10.1128/br.31.2.157-174.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pike R. M., Schulze M. L., Chandler C. H. Agglutinating and precipitating capacity of rabbit anti-Salmonella typhosa gamma G and gamma M-antibodies during prolonged immunization. J Bacteriol. 1966 Oct;92(4):880–886. doi: 10.1128/jb.92.4.880-886.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. ROBBINS J. B., KENNY K., SUTER E. THE ISOLATION AND BIOLOGICAL ACTIVITIES OF RABBIT GAMMA M- AND GAMMA G-ANTI-SALMONELLA TYPHIMURIUM ANTIBODIES. J Exp Med. 1965 Aug 1;122:385–402. doi: 10.1084/jem.122.2.385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tada T., Ishizaka K. Arthus type inflammation with rabbit gammam antibody. J Immunol. 1966 Jan;96(1):112–118. [PubMed] [Google Scholar]
  19. VACCINATION against whooping-cough; relation between protection in children and results of laboratory tests; a report to the Whooping-cough Immunization Committee of the Medical Research Council and to the medical officers of health for Cardiff, Leeds, Leyton, Manchester, Middlesex, Oxford, Poole, Tottenham, Walthamstow, and Wembley. Br Med J. 1956 Aug 25;2(4990):454–462. [PMC free article] [PubMed] [Google Scholar]
  20. Wardlaw A. C., Jakus C. M. Intracerebral mouse protection test for pertussis vaccine. I. Apparent absence of humoral protective antibody under the usual test conditions. Can J Microbiol. 1968 Sep;14(9):989–997. doi: 10.1139/m68-164. [DOI] [PubMed] [Google Scholar]

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