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. 1977 Feb;18(2):156–160. doi: 10.1136/gut.18.2.156

Transmission of rotavirus gastroenteritis from children to a monkey.

J D Mitchell, L A Lambeth, L Sosula, A Murphy, M Albrey
PMCID: PMC1411306  PMID: 192637

Abstract

A pooled suspension of rotavirus was prepared from the stools of eight children with acute non-bacterial gastroenteritis. The suspension was infused into the duodenum and stomach of an infant monkey (Nemestrina macaque). Biopsy samples of duodenal mucosa were taken at several intervals after inoculation, examined by light and electron microscopy, and assayed for lysosomal activity. Virus-like particles were seen within and around microvilli and intracellularly within vesicles as early as 20 minutes after the infusion. On the fourth and fifth days, large lysosomal bodies containing numerous virus-like particles were found within epithelial cells of the duodenal villi. No such particles were seen in the pre-inoculation sample or at days 16 or 25 after infection. The present study would appear to be the first demonstration of the transmission of this human virus to another species.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banfield W. G., Kasnic G., Blackwell J. H. Further observations on the virus of epizootic diarrhea of infant mice. An electron microscopic study. Virology. 1968 Nov;36(3):411–421. doi: 10.1016/0042-6822(68)90166-9. [DOI] [PubMed] [Google Scholar]
  2. Bishop R. F., Davidson G. P., Holmes I. H., Ruck B. J. Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis. Lancet. 1973 Dec 8;2(7841):1281–1283. doi: 10.1016/s0140-6736(73)92867-5. [DOI] [PubMed] [Google Scholar]
  3. Flewett T. H., Bryden A. S., Davies H. Letter: Virus particles in gastroenteritis. Lancet. 1973 Dec 29;2(7844):1497–1497. doi: 10.1016/s0140-6736(73)92760-8. [DOI] [PubMed] [Google Scholar]
  4. Kapikian A. Z., Cline W. L., Mebus C. A., Wyatt R. G., Kalica A. R., James H. D., Jr, VanKirk D., Chanock R. M. New complement-fixation test for the human reovirus-like agent of infantile gastroenteritis. Nebraska calf diarrhea virus used as antigen. Lancet. 1975 May 10;1(7915):1056–1061. doi: 10.1016/s0140-6736(75)91827-9. [DOI] [PubMed] [Google Scholar]
  5. Mitchell J. D., Mitchell J., Peters T. J. Enzyme changes in human small bowel mucosa during culture in vitro. Gut. 1974 Oct;15(10):805–811. doi: 10.1136/gut.15.10.805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Parker J. W., Wakasa H., Lukes R. J. The morphologic and cytochemical demonstration of lysosomes in lymphocytes incubated with phytohemagglutinin by electron microscopy. Lab Invest. 1965 Oct;14(10):1736–1743. [PubMed] [Google Scholar]
  7. Stair E. L., Mebus C. A., Twiehaus M. J., Underdahl N. R. Neonatal calf diarrhea. Electron microscopy of intestines infected with a reovirus-like agent. Vet Pathol. 1973;10(2):155–170. doi: 10.1177/030098587301000208. [DOI] [PubMed] [Google Scholar]
  8. Suzuki H., Konno T. Reovirus-like particles in jejunal mucosa of a Japanese infant with acute infectious non-bacterial gastroenteritis. Tohoku J Exp Med. 1975 Mar;115(3):199–211. doi: 10.1620/tjem.115.199. [DOI] [PubMed] [Google Scholar]
  9. Wyatt R. G., Kapikian A. Z., Thornhill T. S., Sereno M. M., Kim H. W., Chanock R. M. In vitro cultivation in human fetal intestinal organ culture of a reovirus-like agent associated with nonbacterial gastroenteritis in infants and children. J Infect Dis. 1974 Nov;130(5):523–528. doi: 10.1093/infdis/130.5.523. [DOI] [PubMed] [Google Scholar]

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