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. 1972 Sep;13(9):726–731. doi: 10.1136/gut.13.9.726

Synthetic peptides in the diagnosis of exocrine pancreatic insufficiency in animals

A R Imondi, R P Stradley, R Wolgemuth
PMCID: PMC1412369  PMID: 4639406

Abstract

A new approach in the diagnosis of exocrine pancreatic insufficiency has been evaluated in animals. The method involves the oral administration of a chymotrypsin-labile peptide which contains p-aminobenzoic acid (PABA) as a tracer group. In the small bowel in the presence of chymotrypsin, the PABA is split from the peptide and is rapidly absorbed. The amount of PABA (as total aromatic amines) recovered in the urine during the six hours after the dose is used as an index of exocrine pancreatic function. The procedure has been shown to be reliable in detecting surgically induced pancreatic insufficiency in rats, swine, and dogs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ammann R. W., Tagwecher E., Kashiwagi H., Rosenmund H. Diagnostic value of fecal chymotrypsin and trypsin assessment for detection of pancreatic disease. A comparative study. Am J Dig Dis. 1968 Feb;13(2):123–146. doi: 10.1007/BF02232955. [DOI] [PubMed] [Google Scholar]
  2. Ammann R., Kashiwagi H. Pancreatic exocrine insufficiency and proteolytic enzymes in stool. A critical evaluation of a new diagnostic test in various forms of steatorrhea. Helv Med Acta. 1966 Oct;33(3):220–228. [PubMed] [Google Scholar]
  3. CHINN A. B., LAVIK P. S., STITT R. M., BUCKALOO G. W. Use of I131-labeled protein in the diagnosis of pancreatic insufficiency. N Engl J Med. 1952 Dec 4;247(23):877–880. doi: 10.1056/NEJM195212042472302. [DOI] [PubMed] [Google Scholar]
  4. COX A. G. Assessment of the radiotriolein test in steatorrhoea. Br Med J. 1961 Oct 7;2(5257):933–938. doi: 10.1136/bmj.2.5257.933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Christensen H. N., Shwachman H. DETERMINATION OF THE PLASMA GLYCINE AFTER GELATIN FEEDING AS A DIAGNOSTIC PROCEDURE FOR PANCREATIC FIBROSIS. J Clin Invest. 1949 Mar;28(2):319–321. doi: 10.1172/JCI102074. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GROSSMAN M. I. Fecal enzymes of dogs with pancreatic exclusion. Proc Soc Exp Biol Med. 1962 May;110:41–42. doi: 10.3181/00379727-110-27415. [DOI] [PubMed] [Google Scholar]
  7. GROSSMAN M. I. Pancreatic secretion in the rat. Am J Physiol. 1958 Sep;194(3):535–539. doi: 10.1152/ajplegacy.1958.194.3.535. [DOI] [PubMed] [Google Scholar]
  8. Goldberg D. M., Wormsley K. G. The interrelationships of pancreatic enzymes in human duodenal aspirate. Gut. 1970 Oct;11(10):859–866. doi: 10.1136/gut.11.10.859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KENNEDY J. A., KINLOCH J. D. THE IMPURITY OF RADIOIODINATED TRIOLEIN. J Clin Pathol. 1964 Mar;17:160–162. doi: 10.1136/jcp.17.2.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kaffarnik H., Meyer-Bertenrath J. G. Bestimmung von Esteraseaktivitäten in vivo mit dem neuen Substrat Fluoresceindilaurinsäureester. Arzneimittelforschung. 1970 Jun;20(6):754–756. [PubMed] [Google Scholar]
  11. PEKAS J. C., HAYS V. W., THOMPSON A. M. EXCLUSION OF THE EXOCRINE PANCREATIC SECRETION: EFFECT ON DIGESTIBILITY OF SOYBEAN AND MILK PROTEIN BY BABY PIGS AT VARIOUS AGES. J Nutr. 1964 Feb;82:277–286. doi: 10.1093/jn/82.2.277. [DOI] [PubMed] [Google Scholar]
  12. Smith H. W., Finkelstein N., Aliminosa L., Crawford B., Graber M. THE RENAL CLEARANCES OF SUBSTITUTED HIPPURIC ACID DERIVATIVES AND OTHER AROMATIC ACIDS IN DOG AND MAN. J Clin Invest. 1945 May;24(3):388–404. doi: 10.1172/JCI101618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. TUNA N., MANGOLD H. K., MOSSER D. G. Re-evaluation of the I-131-triolein absorption test. Analysis and purification of commercial radioiodinated triolein and clinical studies with pure preparations. J Lab Clin Med. 1963 Apr;61:620–628. [PubMed] [Google Scholar]
  14. Worsmley K. G. Tests of pancreatic function. Scand J Gastroenterol. 1970;5(1):1–3. [PubMed] [Google Scholar]

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