Abstract
Lymphocytes from patients with chronic active hepatitis have been found to be cytotoxic for isolated rabbit hepatocytes. Although this reaction has been shown to be of the antibody-dependent type, no autologous serum was added to the assay system and in the present experiments the source of the antibody has been sought. The failure of puromycin to block the reaction argued against a role for antibody synthesised during the culture period, and the demonstration that normal mononuclear cells could become cytotoxic when preincubated in chronic active hepatitis sera was more in favour of the passive acquisition of an antibody from the circulation. Evidence for the existence of free antibody in the sera reacting with hepatocyte surface antigens came from an additional series of experiments in which it was shown that preincubation of hepatocytes in chronic active hepatitis sera rendered them susceptible to damage by normal mononuclear cells. This effect was almost completely abolished by adding a membrane lipoportein fraction of human liver (LSP) to the sera during the preincubation step, suggesting that the LSP contained those antigens on the hepatocyte surface against which the majority of the antibodies were directed.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cochrane A. M., Moussouros A., Portmann B., McFarlane I. G., Thomson A. D., Eddleston, Williams R. Lymphocyte cytotoxicity for isolated hepatocytes in alcoholic liver disease. Gastroenterology. 1977 May;72(5 Pt 1):918–923. [PubMed] [Google Scholar]
- Cochrane A. M., Moussouros A., Thomsom A. D., Eddleston A. L., Wiiliams R. Antibody-dependent cell-mediated (K cell) cytotoxicity against isolated hepatocytes in chronic active hepatitis. Lancet. 1976 Feb 28;1(7957):441–444. doi: 10.1016/s0140-6736(76)91472-0. [DOI] [PubMed] [Google Scholar]
- David J. R. Suppression of delayed hypersensitivity in vitro by inhibition of protein synthesis. J Exp Med. 1965 Dec 1;122(6):1125–1134. doi: 10.1084/jem.122.6.1125. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Geubel A. P., Keller R. H., Summerskill W. H., Dickson E. R., Tomasi T. B., Shorter R. G. Lymphocyte cytotoxicity and inhibition studied with autologous liver cells: observations in chronic active liver disease and the primary biliary cirrhosis syndrome. Gastroenterology. 1976 Sep;71(3):450–456. [PubMed] [Google Scholar]
- Hughes R. D., Cochrane A. M., Thomson A. D., Murray-Lyon I. M., Williams R. The cytotoxicity of plasma from patients with acute hepatic failure to isolated rabbit hepatocytes. Br J Exp Pathol. 1976 Jun;57(3):348–353. [PMC free article] [PubMed] [Google Scholar]
- Jacques E., De Villers D., Thomas H. C., Sherlock S. Proceedings: Lymphocyte mediated cytotoxicity for Chang liver cells in acute and chronic liver disease. Gut. 1976 May;17(5):389–389. [PubMed] [Google Scholar]
- Jensen D. M., McFarlane I. G., Portmann B. S., Eddleston A. L., Williams R. Detection of antibodies directed against a liver-specific membrane lipoprotein in patients with acute and chronic active hepatitis. N Engl J Med. 1978 Jul 6;299(1):1–7. doi: 10.1056/NEJM197807062990101. [DOI] [PubMed] [Google Scholar]
- McFarlane I. G., Wojcicka B. M., Zucker G. M., Eddleston A. L., Williams R. Purification and characterization of human liver-specific membrane lipoprotein (LSP). Clin Exp Immunol. 1977 Mar;27(3):381–390. [PMC free article] [PubMed] [Google Scholar]
- Rocklin R. E., Meyers O. L., David J. R. An in vitro assay for cellular hypersensitivity in man. J Immunol. 1970 Jan;104(1):95–102. [PubMed] [Google Scholar]
- Thomson A. D., Cochrane M. A., McFarlane I. G., Eddleston A. L., Williams R. Lymphocyte cytotoxicity to isolated hepatocytes in chronic active hepatitis. Nature. 1974 Dec 20;252(5485):721–722. doi: 10.1038/252721a0. [DOI] [PubMed] [Google Scholar]
- Wands J. R., Perrotto J. L., Alpert E., Isselbacher K. J. Cell-mediated immunity in acute and chronic hepatitis. J Clin Invest. 1975 May;55(5):921–929. doi: 10.1172/JCI108021. [DOI] [PMC free article] [PubMed] [Google Scholar]