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. 1973 Aug;14(8):616–626. doi: 10.1136/gut.14.8.616

Studies on the quantitation of immunoglobulin in human intestinal secretions

R R Samson, D B L McClelland, D J C Shearman
PMCID: PMC1412759  PMID: 4582728

Abstract

There is increasing evidence for the importance of the secretory immune system in the gut. In studies of local antibody production it is important to have satisfactory methods for measuring immunoglobulin concentrations and to be aware of the errors which may occur. Studies on immunoglobulin measurement in intestinal secretion by the radial immunodiffusion method are reported, showing the effects of proteolytic digestion, IgA molecular size, and sampling and storage conditions. Because of the presence of monomeric IgA in addition to secretory IgA, there is no satisfactory standard for IgA in gastrointestinal secretions, and only semi-quantitative results can be given. With radial immunodiffusion, IgG and IgM when subjected to tryptic digestion, and IgA when subjected to peptic digestion, may be overestimated because of the presence of fragments of immunoglobulins. In addition, pepsin rapidly destroys IgM and IgG. Both IgM and IgG are unstable in storage. The findings suggest that immunoglobulin concentration measurements in small intestinal aspirates should be interpreted with caution. These problems are also relevant to the detection of specific antibodies in gastrointestinal secretions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown W. R., Savage D. C., Dubois R. S., Alp M. H., Mallory A., Kern F., Jr Intestinal microflora of immunoglobulin-deficient and normal human subjects. Gastroenterology. 1972 Jun;62(6):1143–1152. [PubMed] [Google Scholar]
  2. Douglas A. P., Crabbé P. A., Hobbs J. R. Immunochemical studies on the serum, intestinal secretions and intestinal mucosa in patients with adult celiac disease and other forms of the celiac syndrome. Gastroenterology. 1970 Sep;59(3):414–425. [PubMed] [Google Scholar]
  3. FAHEY J. L., MCKELVEY E. M. QUANTITATIVE DETERMINATION OF SERUM IMMUNOGLOBULINS IN ANTIBODY-AGAR PLATES. J Immunol. 1965 Jan;94:84–90. [PubMed] [Google Scholar]
  4. Falchuk Z. M., Strober W. Increased jejunal immunoglobulin synthesis in patients with nontropical sprue as measured by a solid phase immunoadsorption technique. J Lab Clin Med. 1972 Jun;79(6):1004–1013. [PubMed] [Google Scholar]
  5. Hanson L. A., Holmgren J., Wadsworth C. A radial immune-gel filtration method for characterization and quantitation of macromolecules. Int Arch Allergy Appl Immunol. 1971;40(6):806–819. doi: 10.1159/000230464. [DOI] [PubMed] [Google Scholar]
  6. Loeb P. M., Strober W., Falchuk Z. M., Laster L. Incorporation of L-leucine-14C into immunoglobulins by jejunal biopsies of patients with celiac sprue and other gastrointestinal diseases. J Clin Invest. 1971 Mar;50(3):559–569. doi: 10.1172/JCI106525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McClelland D. B., Finlayson N. D., Samson R. R., Nairn I. M., Shearman D. J. Quantitation of immunoglobulins in gastric juice by electroimmunodiffusion. Gastroenterology. 1971 Apr;60(4):509–514. [PubMed] [Google Scholar]
  8. McClelland D. B., Parkin D. M., Heading R. C., Barnetson R. S., Warwick R. R., Shearman D. J. Small-intestinal immunoglobulin levels in dermatitis herpetiformis. Lancet. 1972 Nov 25;2(7787):1108–1110. doi: 10.1016/s0140-6736(72)92715-8. [DOI] [PubMed] [Google Scholar]
  9. McClelland D. B., Parkin D. M., Samson R. R., Shearman D. J., Reed W. P., Williams R. C., Jr Intestinal immunoglobulins in shigellosis. Gastroenterology. 1972 Apr;62(4):693–695. [PubMed] [Google Scholar]
  10. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  11. Newcomb R. W., Normansell D., Stanworth D. R. A structural study of human exocrine IgA globulin. J Immunol. 1968 Nov;101(5):905–914. [PubMed] [Google Scholar]
  12. Nimmo J., Finlayson N. D., Smith A. F., Shearman D. J. The production of calcium and magnesium during pancreatic function tests in health and disease. Gut. 1970 Feb;11(2):163–166. doi: 10.1136/gut.11.2.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Plaut A. G., Keonil P. Immunoglobulins in human small intestinal fluid. Gastroenterology. 1969 Mar;56(3):522–530. [PubMed] [Google Scholar]
  14. SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]
  15. TOMASI T. B., Jr, ZIGELBAUM S. THE SELECTIVE OCCURENCE OF GAMMA-1A GLOBULINS IN CERTAIN BODY FLUIDS. J Clin Invest. 1963 Oct;42:1552–1560. doi: 10.1172/JCI104840. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tomasi T. B., Jr, Bienenstock J. Secretory immunoglobulins. Adv Immunol. 1968;9:1–96. doi: 10.1016/s0065-2776(08)60441-1. [DOI] [PubMed] [Google Scholar]
  17. Tomasi T. B., Jr Structure and function of mucosal antibodies. Annu Rev Med. 1970;21:281–298. doi: 10.1146/annurev.me.21.020170.001433. [DOI] [PubMed] [Google Scholar]

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