Abstract
A new technique for measuring changes in the architecture of the small intestinal mucosa is described using a modification of the technique of television image analysis to measure the areas occupied by the surface and crypt epithelium in proximal jejunal biopsies. The ratios of these two areas to each other were then calculated. In 12 control mucosae, the mean ratio (±SD) was 2·27 ± 0·41. In 12 untreated flat mucosae, by contrast, the mean ratio was only 0·24 ± 0·07. This ten-fold difference, which was highly significant (p < 0·001), was greater than that for any similar measurements previously reported. This technique is intended for the measurement of changes in the mucosa too small for existing techniques to detect reliably rather than for the diagnosis of coeliac disease.
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- Astaldi G., Conrad M., Ratto L., Costa G. Measurement and features of normal jejunal mucosa by peroral biopsy. Panminerva Med. 1965 Oct;7(10):404–408. [PubMed] [Google Scholar]
- BOLT R. J., PARRISH J. A., FRENCH A. B., POLLARD H. M. ADULT COELIAC DISEASE. HISTOLOGIC RESULTS OF LONG-TERM LOW GLUTEN DIET. Ann Intern Med. 1964 Apr;60:581–586. doi: 10.7326/0003-4819-60-4-581. [DOI] [PubMed] [Google Scholar]
- BUTTERWORTH C. E., Jr, PEREZ-SANTIAGO E. Jejunal biopsies in sprue. Ann Intern Med. 1958 Jan;48(1):8–29. doi: 10.7326/0003-4819-48-1-8. [DOI] [PubMed] [Google Scholar]
- CAMERON A. H., ASTLEY R., HALLOWELL M., RAWSON A. B., MILLER C. G., FRENCH J. M., HUBBLE D. V. Duodeno-jejunal biopsy in the investigation of children with coeliac disease. Q J Med. 1962 Jan;31:125–140. [PubMed] [Google Scholar]
- CHACKO C. J., JOB C. K., JOHNSON S., BAKER S. J. Histopathological changes in the upper jejunum in tropical malabsorption syndrome studied by transoral biopsy. Indian J Pathol Bacteriol. 1961 Oct;4:203–213. [PubMed] [Google Scholar]
- CROSBY W. H., KUGLER H. W. Intraluminal biopsy of the small intestine; the intestinal biopsy capsule. Am J Dig Dis. 1957 May;2(5):236–241. doi: 10.1007/BF02231100. [DOI] [PubMed] [Google Scholar]
- DONIACH I., SHINER M. Duodenal and jejunal biopsies. II. Histology. Gastroenterology. 1957 Jul;33(1):71–86. [PubMed] [Google Scholar]
- Dickson R. A., Paice F., Calnan J. S. The quantitative colour television image analyser. A new method of measuring bone density. J Bone Joint Surg Br. 1973 May;55(2):359–368. [PubMed] [Google Scholar]
- Dunnill M. S., Whitehead R. A method for the quantitation of small intestinal biopsy specimens. J Clin Pathol. 1972 Mar;25(3):243–246. doi: 10.1136/jcp.25.3.243. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans N., Farrow L. J., Harding A., Stewart J. S. New techniques for speeding small intestinal biopsy. Gut. 1970 Jan;11(1):88–89. doi: 10.1136/gut.11.1.88. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FRIC P., LEPSIK J. USE OF ODMAN-LEDIN CATHETER AND SELDINGER WIRE WITH CROSBY CAPSULE. Gut. 1965 Feb;6:101–101. doi: 10.1136/gut.6.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOLMES R., HOURIHANE D. O., BOOTH C. C. Dissecting-microscope appearances of jejunal biopsy specimens from patients with "idiopathic steatorrhoea". Lancet. 1961 Jan 14;1(7168):81–83. doi: 10.1016/s0140-6736(61)92123-7. [DOI] [PubMed] [Google Scholar]
- Hamilton J. R., Lynch M. J., Reilly B. J. Active coeliac disease in childhood. Clinical and laboratory findings of forty-two cases. Q J Med. 1969 Apr;38(150):135–158. [PubMed] [Google Scholar]
- MADANAGOPALAN N., SHINER M., ROWE B. MEASUREMENTS OF SMALL INTESTINAL MUCOSA OBTAINED BY PERORAL BIOPSY. Am J Med. 1965 Jan;38:42–53. doi: 10.1016/0002-9343(65)90158-0. [DOI] [PubMed] [Google Scholar]
- Polak J. M., Stagg B., Pearse A. G. Two types of Zollinger-Ellison syndrome: immunofluorescent, cytochemical and ultrastructural studies of the antral and pancreatic gastrin cells in different clinical states. Gut. 1972 Jul;13(7):501–512. doi: 10.1136/gut.13.7.501. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pollock D. J., Nagle R. E., Jeejeebhoy K. N., Coghill N. F. The effect on jejunal mucosa of withdrawing and adding dietary gluten in cases of idiopathic steatorrhoea. Gut. 1970 Jul;11(7):567–575. doi: 10.1136/gut.11.7.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RUBIN C. E., BRANDBORG L. L., PHELPS P. C., TAYLOR H. C., Jr, MURRAY C. V., STEMLER R., HOWRY C., VOLWILER W. Studies of celiac disease. II. The apparent irreversibility of the proximal intestinal pathology in celiac disease. Gastroenterology. 1960 Apr;38:517–532. [PubMed] [Google Scholar]
- RUBIN C. E., BRANDBORG L. L., PHELPS P. C., TAYLOR H. C., Jr Studies of celiac disease. I. The apparent identical and specific nature of the duodenal and proximal jejunal lesion in celiac disease and idiopathic sprue. Gastroenterology. 1960 Jan;38:28–49. [PubMed] [Google Scholar]
- SHINER M., DONIACH I. Histopathologic studies in steatorrhea. Gastroenterology. 1960 Mar;38:419–440. [PubMed] [Google Scholar]
- SWANSON V. L., THOMASSEN R. W. PATHOLOGY OF THE JEJUNAL MUCOSA IN TROPICAL SPRUE. Am J Pathol. 1965 Apr;46:511–551. [PMC free article] [PubMed] [Google Scholar]
- Stewart J. S., Pollock D. J., Hoffbrand A. V., Mollin D. L., Booth C. C. A study of proximal and distal intestinal structure and absorptive function in idiopathic steatorrhoea. Q J Med. 1967 Jul;36(143):425–444. [PubMed] [Google Scholar]
- THURLBECK W. M., BENSON J. A., Jr, DUDLEY H. R., Jr The histopathologic changes of sprue and their significance. Am J Clin Pathol. 1960 Aug;34:108–117. doi: 10.1093/ajcp/34.2.108. [DOI] [PubMed] [Google Scholar]
- Williams E. D. Automated histoquantitation studies of bone. Proc R Soc Med. 1972 Jun;65(6):539–541. [PMC free article] [PubMed] [Google Scholar]
- YARDLEY J. H., BAYLESS T. M., NORTON J. H., HENDRIX T. R. Celiac disease. A study of the jejunal epithelium before and after a gluten-free diet. N Engl J Med. 1962 Dec 6;267:1173–1179. doi: 10.1056/NEJM196212062672303. [DOI] [PubMed] [Google Scholar]