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. 1974 Feb;15(2):102–111. doi: 10.1136/gut.15.2.102

Immunoreactive gastrin components in human serum

Jens F Rehfeld, Flemming Stadil, Jørgen Vikelsøe
PMCID: PMC1412905  PMID: 4820633

Abstract

The apparent molecular size and charge of immunoreactive gastrin components were studied in sera from patients with pernicious anaemia or gastrinomas (the Zollinger-Ellison syndrome) by Sephadex gel filtration and aminoethylcellulose chromatography. The following serum components were distinguished: (1) a monophasic component I similar in size to proinsulin which was converted into `little' gastrin I by trypsin digestion; (2) a biphasic component II, corresponding to `big' gastrins I and II (Gregory and Tracy); (3) a biphasic component III corresponding to `little' gastrins I and II (Gregory and Tracy); and (4) a biphasic component IV, corresponding to `minigastrins' I and II (Gregory and Tracy). `Big, big' gastrin, a plasma component found in the void volume of the Sephadex G-50 column by Yalow and Berson (1972) was undetectable in the sera investigated. A component in gastrinoma and antral mucosa extracts corresponding in size to `big big' gastrin was detectable by the assay; the `big big' gastrin fraction from gastrinoma tissue was heterogenous, with components of apparent MW 30 000-100 000. It is concluded that serum gastrin circulates in the form of at least four components, of which the three smaller ones are in pairs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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