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. 1974 Dec;15(12):947–952. doi: 10.1136/gut.15.12.947

Gluten-sensitive enteropathy: Synthesis of antigliadin antibody in vitro 1

Z Myron Falchuk, Warren Strober
PMCID: PMC1413076  PMID: 4448409

Abstract

The intestinal mucosa of patients with gluten-sensitive enteropathy responds to gluten challenge in vivo with a striking increase in IgA and IgM synthesis. Whether this increase in immunoglobulin synthesis is due in some part to the production of antigliadin antibodies is examined. Using an affinity chromatography technique it has been demonstrated that in six of seven patients with gluten-sensitive enteropathy approximately half of the net increase in IgA and IgM synthesis occurring after gluten challenge can be attributed to the synthesis of antigliadin antibody. These data strengthen the hypothesis that immunological phenomena are related to the pathogenesis of glutensensitive enteropathy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALARCON SEGOVIA D., HERSKOVIC T., WAKIM K. G., GREEN P. A., SCUDAMORE H. H. PRESENCE OF CIRCULATING ANTIBODIES TO GLUTEN AND MILK FRACTIONS IN PATIENTS WITH NONTROPICAL SPRUE. Am J Med. 1964 Apr;36:485–499. doi: 10.1016/0002-9343(64)90098-1. [DOI] [PubMed] [Google Scholar]
  2. Axén R., Porath J., Ernback S. Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides. Nature. 1967 Jun 24;214(5095):1302–1304. doi: 10.1038/2141302a0. [DOI] [PubMed] [Google Scholar]
  3. BRANDBORG L. L., RUBIN G. E., QUINTON W. E. A multipurpose instrument for suction biopsy of the esophagus, stomach, small bowel, and colon. Gastroenterology. 1959 Jul;37(1):1–16. [PubMed] [Google Scholar]
  4. Cuatrecasas P. Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads. J Biol Chem. 1970 Jun;245(12):3059–3065. [PubMed] [Google Scholar]
  5. FRAZER A. C., FLETCHER R. F., ROSS C. A., SHAW B., SAMMONS H. G., SCHNEIDER R. Gluten-induced enteropathy: the effect of partially digested gluten. Lancet. 1959 Sep 5;2(7097):252–255. doi: 10.1016/s0140-6736(59)92051-3. [DOI] [PubMed] [Google Scholar]
  6. Falchuk Z. M., Gebhard R. L., Sessoms C., Strober W. An in vitro model of gluten-sensitive enteropathy. Effect of gliadin on intestinal epithelial cells of patients with gluten-sensitive enteropathy in organ culture. J Clin Invest. 1974 Feb;53(2):487–500. doi: 10.1172/JCI107582. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Falchuk Z. M., Rogentine G. N., Strober W. Predominance of histocompatibility antigen HL-A8 in patients with gluten-sensitive enteropathy. J Clin Invest. 1972 Jun;51(6):1602–1605. doi: 10.1172/JCI106958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Falchuk Z. M., Strober W. Increased jejunal immunoglobulin synthesis in patients with nontropical sprue as measured by a solid phase immunoadsorption technique. J Lab Clin Med. 1972 Jun;79(6):1004–1013. [PubMed] [Google Scholar]
  9. Ferguson A., Carswell F. Precipitins to dietary proteins in serum and upper intestinal secretions of coeliac children. Br Med J. 1972 Jan 8;1(5792):75–77. doi: 10.1136/bmj.1.5792.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Katz J., Kantor F. S., Herskovic T. Intestinal antibodies to wheat fractions in celiac disease. Ann Intern Med. 1968 Dec;69(6):1149–1153. doi: 10.7326/0003-4819-69-6-1149. [DOI] [PubMed] [Google Scholar]
  12. Kenrick K. G., Walker-Smith J. A. Immunoglobulins and dietary protein antibodies in childhood coeliac disease. Gut. 1970 Aug;11(8):635–640. doi: 10.1136/gut.11.8.635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Loeb P. M., Strober W., Falchuk Z. M., Laster L. Incorporation of L-leucine-14C into immunoglobulins by jejunal biopsies of patients with celiac sprue and other gastrointestinal diseases. J Clin Invest. 1971 Mar;50(3):559–569. doi: 10.1172/JCI106525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stokes P. L., Asquith P., Holmes G. K., Mackintosh P., Cooke W. T. Histocompatibility antigens associated with adult coeliac disease. Lancet. 1972 Jul 22;2(7769):162–164. doi: 10.1016/s0140-6736(72)91330-x. [DOI] [PubMed] [Google Scholar]
  16. TAYLOR K. B., TRUELOVE S. C., THOMSON D. L., WRIGHT R. An immunological study of coeliac disease and idiopathic steatorrhoea. Serological reactions to gluten and milk proteins. Br Med J. 1961 Dec 30;2(5269):1727–1731. doi: 10.1136/bmj.2.5269.1727. [DOI] [PMC free article] [PubMed] [Google Scholar]

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