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. 1963 Sep;4(3):254–260. doi: 10.1136/gut.4.3.254

The absorption of oleic acid in the bile fistula rat

D R Saunders, A M Dawson
PMCID: PMC1413438  PMID: 14058267

Abstract

A technique is described for collecting thoracic duct lymph in a portable glass saddle from an unrestrained rat.

Uniformly labelled oleic acid in various physical states was given to rats with thoracic duct and bile fistulae to study the influence of bile salts on the amount absorbed and on the route of transport and esterification after absorption. It is suggested that in addition to their emulsifying action bile salts may stimulate the esterification of absorbed oleic acid by the intestinal mucosa.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAKER R. D., SEARLE G. W. Bile salt absorption at various levels of rat small intenstine. Proc Soc Exp Biol Med. 1960 Dec;105:521–523. doi: 10.3181/00379727-105-26163. [DOI] [PubMed] [Google Scholar]
  2. BERGSTROM S., BLOMSTRAND R., BORGSTROM B. Route of absorption and distribution of oleic acid and triolein in the rat. Biochem J. 1954 Dec;58(4):600–604. doi: 10.1042/bj0580600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BLOMSTRAND R., DAHLBACK O. L. The fatty acid composition of human thoracic duct lymph lipids. J Clin Invest. 1960 Jul;39:1185–1191. doi: 10.1172/JCI104133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BLOOM B., CHAIKOFF I. L., REINHARDT W. O., ENTENMAN C., DAUBEN W. G. The quantitative significance of the lymphatic pathway in transport of absorbed fatty acids. J Biol Chem. 1950 May;184(1):1–8. [PubMed] [Google Scholar]
  5. BORGSTROM B., DAHLQVIST A., LUNDH G., SJOVALL J. Studies of intestinal digestion and absorption in the human. J Clin Invest. 1957 Oct;36(10):1521–1536. doi: 10.1172/JCI103549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. BORGSTROM B. Investigation on lipid separation methods. Separation of cholesterol esters, glycerides and free fatty acids. Acta Physiol Scand. 1952 Jun 6;25(2-3):111–119. doi: 10.1111/j.1748-1716.1952.tb00863.x. [DOI] [PubMed] [Google Scholar]
  7. BORGSTROM B., TRYDING N. Free fatty acid content of rat thoracic duct lymph during fat absorption. Acta Physiol Scand. 1956 Sep 26;37(2-3):127–133. doi: 10.1111/j.1748-1716.1956.tb01348.x. [DOI] [PubMed] [Google Scholar]
  8. CHAIKOFF I. L., BLOOM B., STEVENS B. P., REINHARDT W. O., DAUBEN W. G. Pentadecanoic acid-5-C14; its absorption and lymphatic transport. J Biol Chem. 1951 Jun;190(2):431–435. [PubMed] [Google Scholar]
  9. DAWSON A. M., ISSELBACHER K. J. Studies on lipid metabolism in the small intestine with observations on the role of bile salts. J Clin Invest. 1960 May;39:730–740. doi: 10.1172/JCI104090. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  11. Frazer A. C. Differentiation in the absorption of olive oil and oleic acid in the rat. J Physiol. 1943 Dec 31;102(3):306–312. doi: 10.1113/jphysiol.1943.sp004036. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HASLEWOOD G. A. Recent developments in our knowledge of bile salts. Physiol Rev. 1955 Jan;35(1):178–196. doi: 10.1152/physrev.1955.35.1.178. [DOI] [PubMed] [Google Scholar]
  13. KIYASU J. Y., BLOOM B., CHAIKOFF I. L. The portal transport of absorbed fatty acids. J Biol Chem. 1952 Nov;199(1):415–419. [PubMed] [Google Scholar]
  14. National Academy of Sciences: Abstracts of Papers Presented at the 96th Annual Meeting, 27-29 April 1959, Washington, D.C. Science. 1959 May 8;129(3358):1282–1290. doi: 10.1126/science.129.3358.1282. [DOI] [PubMed] [Google Scholar]
  15. OLSON J. A. The conversion of radioactive beta-carotene into vitamin A by the rat intestine in vivo. J Biol Chem. 1961 Feb;236:349–356. [PubMed] [Google Scholar]
  16. PALAY S. L., KARLIN L. J. An electron microscopic study of the intestinal villus. II. The pathway of fat absorption. J Biophys Biochem Cytol. 1959 May 25;5(3):373–384. doi: 10.1083/jcb.5.3.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. PESSOA V. C., KIM K. S., IVY A. C. Fat absorption in absence of bile and pancreatic juice. Am J Physiol. 1953 Aug;174(2):209–218. doi: 10.1152/ajplegacy.1953.174.2.209. [DOI] [PubMed] [Google Scholar]
  18. SENIOR J. R., ISSELBACHER K. J. Formation of higher glycerides from monopalmitin and palmityl-CoA by microsomes of rat intestinal mucosa. Biochem Biophys Res Commun. 1961 Nov 29;6:274–278. doi: 10.1016/0006-291x(61)90377-1. [DOI] [PubMed] [Google Scholar]
  19. VAHOUNY G. V., WOO C. H., TREADWELL C. R. Quantitative effects of bile salt and fatty acid on cholesterol absorption in the rat. Am J Physiol. 1958 Apr;193(1):41–46. doi: 10.1152/ajplegacy.1958.193.1.41. [DOI] [PubMed] [Google Scholar]
  20. VAN ZYL A. Note on the effects of thyroidectomy and thyroid hormone administration on the concentration of bile cholesterol and cholic acid. J Endocrinol. 1957 Dec;16(2):213–216. doi: 10.1677/joe.0.0160213. [DOI] [PubMed] [Google Scholar]
  21. WEINER I. M., LACK L. Absorption of bile salts from the small intestine in vivo. Am J Physiol. 1962 Jan;202:155–157. doi: 10.1152/ajplegacy.1962.202.1.155. [DOI] [PubMed] [Google Scholar]

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