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. 1994 Dec;83(4):513–516.

Murine B-cell activation via CD38 and protein tyrosine phosphorylation.

P A Kirkham 1, L Santos-Argumedo 1, M M Harnett 1, R M Parkhouse 1
PMCID: PMC1415074  PMID: 7875731

Abstract

CD38 has been implicated in the regulation of both proliferation and rescue from apoptosis of B cells. The signalling events associated with CD38-mediated activation of murine B cells are, as yet, not well defined but it is clear that ligation of CD38 by a mitogenic antibody, NIMR-5, induces a calcium influx in resting B cells. Interestingly, however, cross-linking of CD38 does not mobilize intracellular stores of calcium. We now provide a rationale for these findings by demonstrating that CD38 is not coupled to the generation of inositol phosphates in resting B cells. We do, however, show that CD38 ligation stimulates one, or more, protein tyrosine kinase activities which may play a central role in the transduction of CD38-mediated signals leading to B-cell activation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akiyama T., Ishida J., Nakagawa S., Ogawara H., Watanabe S., Itoh N., Shibuya M., Fukami Y. Genistein, a specific inhibitor of tyrosine-specific protein kinases. J Biol Chem. 1987 Apr 25;262(12):5592–5595. [PubMed] [Google Scholar]
  2. Baixeras E., Kroemer G., Cuende E., Márquez C., Boscá L., Alés Martínez J. E., Martínez C. Signal transduction pathways involved in B-cell induction. Immunol Rev. 1993 Apr;132:5–47. doi: 10.1111/j.1600-065x.1993.tb00836.x. [DOI] [PubMed] [Google Scholar]
  3. Burkhardt A. L., Brunswick M., Bolen J. B., Mond J. J. Anti-immunoglobulin stimulation of B lymphocytes activates src-related protein-tyrosine kinases. Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7410–7414. doi: 10.1073/pnas.88.16.7410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Campbell M. A., Sefton B. M. Association between B-lymphocyte membrane immunoglobulin and multiple members of the Src family of protein tyrosine kinases. Mol Cell Biol. 1992 May;12(5):2315–2321. doi: 10.1128/mcb.12.5.2315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gelman L., Deterre P., Gouy H., Boumsell L., Debré P., Bismuth G. The lymphocyte surface antigen CD38 acts as a nicotinamide adenine dinucleotide glycohydrolase in human T lymphocytes. Eur J Immunol. 1993 Dec;23(12):3361–3364. doi: 10.1002/eji.1830231245. [DOI] [PubMed] [Google Scholar]
  6. Harada N., Santos-Argumedo L., Chang R., Grimaldi J. C., Lund F. E., Brannan C. I., Copeland N. G., Jenkins N. A., Heath A. W., Parkhouse R. M. Expression cloning of a cDNA encoding a novel murine B cell activation marker. Homology to human CD38. J Immunol. 1993 Sep 15;151(6):3111–3118. [PubMed] [Google Scholar]
  7. Harnett M. M., Klaus G. G. G protein coupling of antigen receptor-stimulated polyphosphoinositide hydrolysis in B cells. J Immunol. 1988 May 1;140(9):3135–3139. [PubMed] [Google Scholar]
  8. Hutchcroft J. E., Harrison M. L., Geahlen R. L. B lymphocyte activation is accompanied by phosphorylation of a 72-kDa protein-tyrosine kinase. J Biol Chem. 1991 Aug 15;266(23):14846–14849. [PubMed] [Google Scholar]
  9. Jongstra J., Misener V. Developmental maturation of the B-cell antigen receptor. Immunol Rev. 1993 Apr;132:107–123. doi: 10.1111/j.1600-065x.1993.tb00839.x. [DOI] [PubMed] [Google Scholar]
  10. Justement L. B., Wienands J., Hombach J., Reth M., Cambier J. C. Membrane IgM and IgD molecules fail to transduce Ca2+ mobilizing signals when expressed on differentiated B lineage cells. J Immunol. 1990 May 1;144(9):3272–3280. [PubMed] [Google Scholar]
  11. Khan A. A., Steiner J. P., Klein M. G., Schneider M. F., Snyder S. H. IP3 receptor: localization to plasma membrane of T cells and cocapping with the T cell receptor. Science. 1992 Aug 7;257(5071):815–818. doi: 10.1126/science.1323146. [DOI] [PubMed] [Google Scholar]
  12. Mire-Sluis A. R., Thorpe R. Interleukin-4 proliferative signal transduction involves the activation of a tyrosine-specific phosphatase and the dephosphorylation of an 80-kDa protein. J Biol Chem. 1991 Sep 25;266(27):18113–18118. [PubMed] [Google Scholar]
  13. Rawlings D. J., Saffran D. C., Tsukada S., Largaespada D. A., Grimaldi J. C., Cohen L., Mohr R. N., Bazan J. F., Howard M., Copeland N. G. Mutation of unique region of Bruton's tyrosine kinase in immunodeficient XID mice. Science. 1993 Jul 16;261(5119):358–361. doi: 10.1126/science.8332901. [DOI] [PubMed] [Google Scholar]
  14. Santos-Argumedo L., Teixeira C., Preece G., Kirkham P. A., Parkhouse R. M. A B lymphocyte surface molecule mediating activation and protection from apoptosis via calcium channels. J Immunol. 1993 Sep 15;151(6):3119–3130. [PubMed] [Google Scholar]
  15. Sorrentino V., Volpe P. Ryanodine receptors: how many, where and why? Trends Pharmacol Sci. 1993 Mar;14(3):98–103. doi: 10.1016/0165-6147(93)90072-r. [DOI] [PubMed] [Google Scholar]
  16. Takasawa S., Nata K., Yonekura H., Okamoto H. Cyclic ADP-ribose in insulin secretion from pancreatic beta cells. Science. 1993 Jan 15;259(5093):370–373. doi: 10.1126/science.8420005. [DOI] [PubMed] [Google Scholar]
  17. Takata M., Sabe H., Hata A., Inazu T., Homma Y., Nukada T., Yamamura H., Kurosaki T. Tyrosine kinases Lyn and Syk regulate B cell receptor-coupled Ca2+ mobilization through distinct pathways. EMBO J. 1994 Mar 15;13(6):1341–1349. doi: 10.1002/j.1460-2075.1994.tb06387.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tsukada S., Saffran D. C., Rawlings D. J., Parolini O., Allen R. C., Klisak I., Sparkes R. S., Kubagawa H., Mohandas T., Quan S. Deficient expression of a B cell cytoplasmic tyrosine kinase in human X-linked agammaglobulinemia. Cell. 1993 Jan 29;72(2):279–290. doi: 10.1016/0092-8674(93)90667-f. [DOI] [PubMed] [Google Scholar]
  19. Yamanashi Y., Kakiuchi T., Mizuguchi J., Yamamoto T., Toyoshima K. Association of B cell antigen receptor with protein tyrosine kinase Lyn. Science. 1991 Jan 11;251(4990):192–194. doi: 10.1126/science.1702903. [DOI] [PubMed] [Google Scholar]
  20. Yellen A. J., Glenn W., Sukhatme V. P., Cao X. M., Monroe J. G. Signaling through surface IgM in tolerance-susceptible immature murine B lymphocytes. Developmentally regulated differences in transmembrane signaling in splenic B cells from adult and neonatal mice. J Immunol. 1991 Mar 1;146(5):1446–1454. [PubMed] [Google Scholar]
  21. Zocchi E., Franco L., Guida L., Benatti U., Bargellesi A., Malavasi F., Lee H. C., De Flora A. A single protein immunologically identified as CD38 displays NAD+ glycohydrolase, ADP-ribosyl cyclase and cyclic ADP-ribose hydrolase activities at the outer surface of human erythrocytes. Biochem Biophys Res Commun. 1993 Nov 15;196(3):1459–1465. doi: 10.1006/bbrc.1993.2416. [DOI] [PubMed] [Google Scholar]

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