Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1995 Mar;84(3):355–359.

Major histocompatibility complex control of the class of the immune response to the hapten trinitrophenyl.

F Dieli 1, G L Asherson 1, C T Bonanno 1, G Sireci 1, A Salerno 1
PMCID: PMC1415122  PMID: 7751016

Abstract

This paper investigates major histocompatibility complex (MHC) regulation of the class of the immune response given in vitro and in vivo following immunization of the congenic BALB/k (H-2k) and BALB/c (H-2d) mice with the hapten trinitrophenyl (TNP). TNP-immune lymph node cells from BALB/k mice produced high levels of interferon-gamma (IFN-gamma), interleukin-5 (IL-5) and IL-2 when stimulated with TNP-antigen-presenting cells (APC) in vitro, while TNP-immune lymph node cells from BALB/c mice produced very low levels of these cytokines. No significant difference was found in antigen-specific production of IL-3, IL-4 and tumour necrosis factor-alpha (TNF-alpha). There was a strong correlation between the pattern of cytokine production in vitro and the secondary antibody production in vivo. Sera from BALB/k mice had anti-TNP IgG2a, IgG2b and IgG3 levels threefold greater, and anti-TNP IgA levels eightfold greater, than BALB/c mice. The level of specific IgG1 and IgE was only marginally raised in BALB/k mice. In contrast to these strain differences in cytokine and antibody production, there was no difference in two measures of cellular immunity: contact sensitivity in vivo and antigen-specific lymphocyte response in vitro. Our results suggest that there is a good correlation between the production of cytokines in vitro and antibody response in vivo, but not with measures of cellular immunity. Moreover, this MHC control of the class of the immune response to TNP does not fit into the T-helper type-1 (Th1)-Th2 paradigm.

Full text

PDF
359

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asherson G. L., Dieli F., Gautam Y., Siew L. K., Zembala M. Major histocompatibility complex regulation of the class of the immune response: the H-2d haplotype determines poor interferon-gamma response to several antigens. Eur J Immunol. 1990 Jun;20(6):1305–1310. doi: 10.1002/eji.1830200616. [DOI] [PubMed] [Google Scholar]
  2. Askenase P. W., Asherson G. L. Contact sensitivity to oxazolone in the mouse. VIII. Demonstration of several classes of antibody in the sera of contact sensitized and unimmunized mice by a simplified antiglobulin assay. Immunology. 1972 Sep;23(3):289–298. [PMC free article] [PubMed] [Google Scholar]
  3. Burstein H. J., Tepper R. I., Leder P., Abbas A. K. Humoral immune functions in IL-4 transgenic mice. J Immunol. 1991 Nov 1;147(9):2950–2956. [PubMed] [Google Scholar]
  4. Dieli F., Sireci G., Lio D., Bonanno C. T., Salerno A. Major histocompatibility complex regulation of interleukin-5 production in the mouse. Eur J Immunol. 1993 Nov;23(11):2897–2902. doi: 10.1002/eji.1830231125. [DOI] [PubMed] [Google Scholar]
  5. Ellis J., Chain B. M., Davies D. H., Ibrahim M. A., Katz D. R., Kaye P. M., Lightstone E. Antigen presentation by dendritic cells provides optimal stimulation for the production of interleukin (IL) 2, IL 4 and interferon-gamma by allogeneic T cells. Eur J Immunol. 1991 Nov;21(11):2803–2809. doi: 10.1002/eji.1830211123. [DOI] [PubMed] [Google Scholar]
  6. Finkelman F. D., Holmes J., Katona I. M., Urban J. F., Jr, Beckmann M. P., Park L. S., Schooley K. A., Coffman R. L., Mosmann T. R., Paul W. E. Lymphokine control of in vivo immunoglobulin isotype selection. Annu Rev Immunol. 1990;8:303–333. doi: 10.1146/annurev.iy.08.040190.001511. [DOI] [PubMed] [Google Scholar]
  7. Fong T. A., Mosmann T. R. The role of IFN-gamma in delayed-type hypersensitivity mediated by Th1 clones. J Immunol. 1989 Nov 1;143(9):2887–2893. [PubMed] [Google Scholar]
  8. Janeway C. A., Jr, Carding S., Jones B., Murray J., Portoles P., Rasmussen R., Rojo J., Saizawa K., West J., Bottomly K. CD4+ T cells: specificity and function. Immunol Rev. 1988 Jan;101:39–80. doi: 10.1111/j.1600-065x.1988.tb00732.x. [DOI] [PubMed] [Google Scholar]
  9. Mohler K. M., Butler L. D. Differential production of IL-2 and IL-4 mRNA in vivo after primary sensitization. J Immunol. 1990 Sep 15;145(6):1734–1739. [PubMed] [Google Scholar]
  10. Mosmann T. R., Cherwinski H., Bond M. W., Giedlin M. A., Coffman R. L. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol. 1986 Apr 1;136(7):2348–2357. [PubMed] [Google Scholar]
  11. Piguet P. F., Grau G. E., Hauser C., Vassalli P. Tumor necrosis factor is a critical mediator in hapten induced irritant and contact hypersensitivity reactions. J Exp Med. 1991 Mar 1;173(3):673–679. doi: 10.1084/jem.173.3.673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schwartz R. H. Immune response (Ir) genes of the murine major histocompatibility complex. Adv Immunol. 1986;38:31–201. doi: 10.1016/s0065-2776(08)60006-1. [DOI] [PubMed] [Google Scholar]
  13. Schwartz R. H. T-lymphocyte recognition of antigen in association with gene products of the major histocompatibility complex. Annu Rev Immunol. 1985;3:237–261. doi: 10.1146/annurev.iy.03.040185.001321. [DOI] [PubMed] [Google Scholar]
  14. Shimonkevitz R., Kappler J., Marrack P., Grey H. Antigen recognition by H-2-restricted T cells. I. Cell-free antigen processing. J Exp Med. 1983 Aug 1;158(2):303–316. doi: 10.1084/jem.158.2.303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Skoglund C., Scheynius A., Holmdahl R., Van der Meide P. H. Enhancement of DTH reaction and inhibition of the expression of class II transplantation antigens by in vivo treatment with antibodies against gamma-interferon. Clin Exp Immunol. 1988 Mar;71(3):428–432. [PMC free article] [PubMed] [Google Scholar]
  16. Snapper C. M., Paul W. E. Interferon-gamma and B cell stimulatory factor-1 reciprocally regulate Ig isotype production. Science. 1987 May 22;236(4804):944–947. doi: 10.1126/science.3107127. [DOI] [PubMed] [Google Scholar]
  17. Thomas J. W., Danho W., Bullesbach E., Föhles J., Rosenthal A. S. Immune response gene control of determinant selection. III. Polypeptide fragments of insulin are differentially recognized by T but not by B cells in insulin immune guinea pigs. J Immunol. 1981 Mar;126(3):1095–1100. [PubMed] [Google Scholar]
  18. Thomas W. R., Asherson G. L., Watkins M. C. Reaginic antibody produced in mice with contact sensitivity. J Exp Med. 1976 Nov 2;144(5):1386–1390. doi: 10.1084/jem.144.5.1386. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Thomson J. A., Troutt A. B., Kelso A. Contact sensitization to oxazolone: involvement of both interferon-gamma and interleukin-4 in oxazolone-specific Ig and T-cell responses. Immunology. 1993 Feb;78(2):185–192. [PMC free article] [PubMed] [Google Scholar]
  20. Yewdell J. W., Bennink J. R. The binary logic of antigen processing and presentation to T cells. Cell. 1990 Jul 27;62(2):203–206. doi: 10.1016/0092-8674(90)90356-j. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES