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. 1980 Aug;21(8):675–682. doi: 10.1136/gut.21.8.675

Lymphocyte subpopulations of intestinal mucosa in inflammatory bowel disease.

O E Eade, S S Andre-Ukena, C Moulton, B MacPherson, W L Beeken
PMCID: PMC1419116  PMID: 6968706

Abstract

Lymphocyte subpopulations in peripheral blood (PBL) and intestinal mucosa (IML) of 10 patients with inflammatory bowel disease (IBD) were compared with those of 11 non-IBD controls. PBL were separated on Ficoll/hypaque gradients, and IML were isolated by incubation in dithiothreitol, EDTA, and collagenase. These methods yielded cells of good viability and with intact HLA A and B-antigens. T-cells, identified by neuraminidase-treated sheep RBC rosettes and non-specific esterase staining, comprised approximately 91% of the IML from normal mucosa of all groups. B-cells, identified by erythrocyte-antibody-complement rosettes and surface immunoglobulins, were only 7% of these IML populations. Cell yields were two-fold or more greater from abnormal IBD mucosa, with T-cells ranging from 55 to 95% and B-cells from 2 to 36%. The percentage of Fc receptor bearing cells was low in all specimens. By these methods, T-lymphocytes predominated in intestinal mucosa of both IBD and non-IBD patients, but there is marked increase in the percentage of B-cells isolated from abnormal mucosa in IBD.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amos D. B., Bashir H., Boyle W., MacQueen M., Tiilikainen A. A simple micro cytotoxicity test. Transplantation. 1969 Mar;7(3):220–223. doi: 10.1097/00007890-196903000-00023. [DOI] [PubMed] [Google Scholar]
  2. Arnaud-Battandier F., Bundy B. M., O'Neill M., Bienenstock J., Nelson D. L. Cytotoxic activities of gut mucosal lymphoid cells in guinea pigs. J Immunol. 1978 Sep;121(3):1059–1065. [PubMed] [Google Scholar]
  3. Baklien K., Brandtzaeg P. Comparative mapping of the local distribution of immunoglobulin-containing cells in ulcerative colitis and Crohn's disease of the colon. Clin Exp Immunol. 1975 Nov;22(2):197–209. [PMC free article] [PubMed] [Google Scholar]
  4. Barr R. D., Allardyce M. Cytochemical identification of human T-lymphocytes. Can Med Assoc J. 1978 May 20;118(10):1279-80, 1285. [PMC free article] [PubMed] [Google Scholar]
  5. Bookman M. A., Bull D. M. Characteristics of isolated intestinal mucosal lymphoid cells in inflammatory bowel disease. Gastroenterology. 1979 Sep;77(3):503–510. [PubMed] [Google Scholar]
  6. Brandtzaeg P., Baklien K. Immunohistochemical studies of the formation and epithelial transport of immunoglobulins in normal and diseased human intestinal mucosa. Scand J Gastroenterol Suppl. 1976;36:1–45. [PubMed] [Google Scholar]
  7. Breucha G., Riethmüller G. Letter: Intestinal lymphocytes in Crohn's disease. Lancet. 1975 Apr 26;1(7913):976–976. doi: 10.1016/s0140-6736(75)92037-1. [DOI] [PubMed] [Google Scholar]
  8. Breucha G., Riethmüller G., Rieber E. P. Isolierung und immunologische Charakterisierung von Lymphocyten aus humanen Appendices. Klin Wochenschr. 1975 Dec 15;53(24):1155–1159. doi: 10.1007/BF01476455. [DOI] [PubMed] [Google Scholar]
  9. Bull D. M., Bookman M. A. Isolation and functional characterization of human intestinal mucosal lymphoid cells. J Clin Invest. 1977 May;59(5):966–974. doi: 10.1172/JCI108719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Clancy R. Isolation and kinetic characteristics of mucosal lymphocytes in Crohn's Disease. Gastroenterology. 1976 Feb;70(2):177–180. [PubMed] [Google Scholar]
  11. Clancy R., Pucci A. Absence of K cells in human gut mucosa. Gut. 1978 Apr;19(4):273–276. doi: 10.1136/gut.19.4.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gelfand E. W., Shore A., Green B., Lin M. T., Dosch H. M. The E-rosette assay: a cautionary note. Clin Immunol Immunopathol. 1979 Jan;12(1):119–123. doi: 10.1016/0090-1229(79)90117-x. [DOI] [PubMed] [Google Scholar]
  13. Goodacre R., Davidson R., Singal D., Bienenstock J. Morphologic and functional characteristics of human intestinal lymphoid cells isolated by a mechanical technique. Gastroenterology. 1979 Feb;76(2):300–308. [PubMed] [Google Scholar]
  14. Grossi C. E., Webb S. R., Zicca A., Lydyard P. M., Moretta L., Mingari M. C., Cooper M. D. Morphological and histochemical analyses of two human T-cell subpopulations bearing receptors for IgM or IgG. J Exp Med. 1978 May 1;147(5):1405–1417. doi: 10.1084/jem.147.5.1405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Horton M. A., Burman J. F. Reversible C3 hypocomplementaemia in megaloblastic anaemia due to vitamin B12 deficiency. Br J Haematol. 1977 May;36(1):23–27. doi: 10.1111/j.1365-2141.1977.tb05751.x. [DOI] [PubMed] [Google Scholar]
  16. Horwitz D. A., Allison A. C., Ward P., Kight N. Identification of human mononuclear leucocyte populations by esterase staining. Clin Exp Immunol. 1977 Nov;30(2):289–298. [PMC free article] [PubMed] [Google Scholar]
  17. Li C. Y., Yam L. T., Crosby W. H. Histochemical characterization of cellular and structural elements of the human spleen. J Histochem Cytochem. 1972 Dec;20(12):1049–1058. doi: 10.1177/20.12.1049. [DOI] [PubMed] [Google Scholar]
  18. Manconi P. E., Marrosu M. G., Paghi L., Correale G., Zaccheo D. Alpha-naphthyl acetate esterase activity in human lymphocytes: distribution in lymphocyte subpopulations and in mitogen-activated cells. Scand J Immunol. 1979;9(2):99–104. doi: 10.1111/j.1365-3083.1979.tb02711.x. [DOI] [PubMed] [Google Scholar]
  19. Mavligit G. M., Jubert A. V., Gutterman J. U., McBride C. M., Hersh E. M. Immune reactivity of lymphoid tissues adjacent to carcinoma of the ascending colon. Surg Gynecol Obstet. 1974 Sep;139(3):409–412. [PubMed] [Google Scholar]
  20. Meijer C. J., Bosman F. T., Lindeman J. Evidence for predominant involvement of the B-cell system in the inflammatory process in Crohn's disease. Scand J Gastroenterol. 1979;14(1):21–32. doi: 10.3109/00365527909179842. [DOI] [PubMed] [Google Scholar]
  21. Meuwissen S. G., Feltkamp-Vroom T. M., De La Rivière A. B., Von Dem Borne A. E., Tytgat G. N. Analysis of the lympho-plasmacytic infiltrate in Crohn's disease with special reference to identification of lymphocyte-subpopulations. Gut. 1976 Oct;17(10):770–780. doi: 10.1136/gut.17.10.770. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Mueller J., Brun del Re G., Buerki H., Keller H. U., Hess M. W., Cottier H. Nonspecific acid esterase activity: a criterion for differentiation of T and B lymphocytes in mouse lymph nodes. Eur J Immunol. 1975 Apr;5(4):270–274. doi: 10.1002/eji.1830050411. [DOI] [PubMed] [Google Scholar]
  23. Obrist R., Albrecht R., Nagel G. A. Identification of lymphocyte subpopulations by simultaneous E-rosette formation and unspecific acid esterase staining. Experientia. 1978 May 15;34(5):660–661. doi: 10.1007/BF01937018. [DOI] [PubMed] [Google Scholar]
  24. Ranki A., Tötterman T. H., Häyry P. Identification of mouse T and B lymphocytes from cytocentrifuged cell smears. Clin Exp Immunol. 1976 Dec;26(3):632–640. [PMC free article] [PubMed] [Google Scholar]
  25. Rudzik O., Bienenstock J. Isolation and characteristics of gut mucosal lymphocytes. Lab Invest. 1974 Mar;30(3):260–266. [PubMed] [Google Scholar]
  26. Shevach E. M., Herberman R., Frank M. M., Green I. Receptors for complement and immunoglobulin on human leukemic cells and human lymphoblastoid cell lines. J Clin Invest. 1972 Aug;51(8):1933–1938. doi: 10.1172/JCI106999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Strickland R. G., Husby G., Black W. C., Williams R. C., Jr Peripheral blood and intestinal lymphocyte sub-populations in Crohn's disease. Gut. 1975 Nov;16(11):847–853. doi: 10.1136/gut.16.11.847. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Weiner M. S., Bianco C., Nussenzweig V. Enhanced binding of neuraminidase-treated sheep erythrocytes to human T lymphocytes. Blood. 1973 Dec;42(6):939–946. [PubMed] [Google Scholar]

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