Skip to main content
PMC home page
Gut logoLink to Gut
. 1981 May;22(5):376–382. doi: 10.1136/gut.22.5.376

Regional blood flow and the localisation of lymphoblasts in the small intestine of the mouse: effect of an elemental diet.

C A Ottaway, D M Parrott
PMCID: PMC1419247  PMID: 6972889

Abstract

To test the hypothesis that food antigens influence the in vivo migration of lymphoblasts to the small intestine, the effect of an elemental diet (Vivonex) on the distribution of lymphoblasts within the small intestine of mice has been examined. Viable lymphoblasts from the mesenteric nodes of conventionally fed animals were labelled in vitro and given intravenously to recipient mice fed either a standard diet or elemental diet. The localisation of these cells within the small intestine was altered in the animals fed the elemental diet but only in the distal half of the small intestine. The relationship of the localisation of blast cells to the delivery of cardiac output along the small intestine was examined by assessing cell localisation in conjunction with the distribution of an isotopic indicator (86RbC1). The results show that the pattern of localisation of lymphoblasts within the small intestine is related to the probability that they will be delivered to different regions by the blood stream. Therefore, the alterations in blast localisation in the small intestine of animals of the elemental diet can be viewed as a consequence of changes in the perfusion of the distal small intestine. These results do not support the concept that antigens directly influence the efficiency with which blast cells migrate into the intestinal mucosa.

Full text

PDF
377

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen W. D., Porter P. The relative frequencies and distribution of immunoglobulin-bearing cells in the intestinal mucosa of neonatal and weaned pigs and their significance in the development of secretory immunity. Immunology. 1977 May;32(5):819–824. [PMC free article] [PubMed] [Google Scholar]
  2. Brandtzaeg P., Baklien K. Immunoglobulin-producing cells in the intestine in health and disease. Clin Gastroenterol. 1976 May;5(2):251–269. [PubMed] [Google Scholar]
  3. Crabbé P. A., Bazin H., Eyssen H., Heremans J. F. The normal microbial flora as a major stimulus for proliferation of plasma cells synthesizing IgA in the gut. The germ-free intestinal tract. Int Arch Allergy Appl Immunol. 1968;34(4):362–375. doi: 10.1159/000230130. [DOI] [PubMed] [Google Scholar]
  4. Craig S. W., Cebra J. J. Peyer's patches: an enriched source of precursors for IgA-producing immunocytes in the rabbit. J Exp Med. 1971 Jul 1;134(1):188–200. doi: 10.1084/jem.134.1.188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ferguson A., Logan R. F., MacDonald T. T. Increased mucosal damage during parasite infection in mice fed an elemental diet. Gut. 1980 Jan;21(1):37–43. doi: 10.1136/gut.21.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GOWANS J. L., KNIGHT E. J. THE ROUTE OF RE-CIRCULATION OF LYMPHOCYTES IN THE RAT. Proc R Soc Lond B Biol Sci. 1964 Jan 14;159:257–282. doi: 10.1098/rspb.1964.0001. [DOI] [PubMed] [Google Scholar]
  7. Greaves M. F., Brown G. Purification of human T and B lymphocytes. J Immunol. 1974 Jan;112(1):420–423. [PubMed] [Google Scholar]
  8. Guy-Grand D., Griscelli C., Vassalli P. The gut-associated lymphoid system: nature and properties of the large dividing cells. Eur J Immunol. 1974 Jun;4(6):435–443. doi: 10.1002/eji.1830040610. [DOI] [PubMed] [Google Scholar]
  9. Hall J. G., Hopkins J., Orlans E. Studies on the lymphocytes of sheep. III. Destination of lymph-borne immunoblasts in relation to their tissue of origin. Eur J Immunol. 1977 Jan;7(1):30–37. doi: 10.1002/eji.1830070108. [DOI] [PubMed] [Google Scholar]
  10. Halstead T. E., Hall J. G. The homing of lymph-borne immunoblasts to the small gut of neonatal rats. Transplantation. 1972 Sep;14(3):339–346. doi: 10.1097/00007890-197209000-00009. [DOI] [PubMed] [Google Scholar]
  11. Husband A. J., Gowans J. L. The origin and antigen-dependent distribution of IgA-containing cells in the intestine. J Exp Med. 1978 Nov 1;148(5):1146–1160. doi: 10.1084/jem.148.5.1146. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Husband A. J., Lascelles A. K. The origin of antibody in intestinal secretion of sheep. Aust J Exp Biol Med Sci. 1974 Oct;52(5):791–799. doi: 10.1038/icb.1974.78. [DOI] [PubMed] [Google Scholar]
  13. Lange S., Nygren H., Svennerholm A. M., Holmgren J. Antitoxic cholera immunity in mice: influence of antigen deposition on antitoxin-containing cells and protective immunity in different parts of the intestine. Infect Immun. 1980 Apr;28(1):17–23. doi: 10.1128/iai.28.1.17-23.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ottaway C. A., Parrott D. M. Regional blood flow and its relationship to lymphocyte and lymphoblast traffic during a primary immune reaction. J Exp Med. 1979 Aug 1;150(2):218–230. doi: 10.1084/jem.150.2.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ottaway C. A., Parrott D. M. Regional blood flow and the localization of lymphoblasts in the small intestine of the mouse. I. Examination of normal small intestine. Immunology. 1980 Dec;41(4):955–961. [PMC free article] [PubMed] [Google Scholar]
  16. Parrott D. M., Ferguson A. Selective migration of lymphocytes within the mouse small intestine. Immunology. 1974 Mar;26(3):571–588. [PMC free article] [PubMed] [Google Scholar]
  17. Porter P., Noakes D. E., Allen W. D. Intestinal secretion of immunoglobulins in the preruminant calf. Immunology. 1972 Sep;23(3):299–312. [PMC free article] [PubMed] [Google Scholar]
  18. Rose M. L., Parrott D. M., Bruce R. G. I. Effect of Trichinella spiralis infection on the migration of mesenteric lymphoblasts and mesenteric T lymphoblasts in syngeneic mice. Immunology. 1976 Nov;31(5):723–730. [PMC free article] [PubMed] [Google Scholar]
  19. Rose M. L., Parrott D. M., Bruce R. G. Migration of lymphoblasts to the small intestine. II. Divergent migration of mesenteric and peripheral immunoblasts to sites of inflammation in the mouse. Cell Immunol. 1976 Nov;27(1):36–46. doi: 10.1016/0008-8749(76)90151-9. [DOI] [PubMed] [Google Scholar]
  20. Russel R. I. Elemental diets. Gut. 1975 Jan;16(1):68–79. doi: 10.1136/gut.16.1.68. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. SAPIRSTEIN L. A. Regional blood flow by fractional distribution of indicators. Am J Physiol. 1958 Apr;193(1):161–168. doi: 10.1152/ajplegacy.1958.193.1.161. [DOI] [PubMed] [Google Scholar]

Articles from Gut are provided here courtesy of BMJ Publishing Group

RESOURCES