Skip to main content
NCBI home page
Gut logoLink to Gut
. 1981 Oct;22(10):823–826. doi: 10.1136/gut.22.10.823

Cytopathic effects in cultures inoculated with material from Crohn's disease.

C O Moráin, H Prestage, P Harrison, A J Levi, D A Tyrrell
PMCID: PMC1419430  PMID: 7297913

Abstract

Rectal biopsy homogenates, faecal samples, and leucocyte rich plasma obtained from 39 patients were inoculated into tissue cultures. A non-transmissible cytopathic effect was observed in cultures inoculated with material from patients with Crohn's disease. In a blind study this cytopathic effect was observed in three specimens from three patients with Crohn's disease, in six specimens from four patients with ulcerative colitis, and six specimens from five patients with upper gastrointestinal disease. The cytopathic effect is unlikely to be associated with the bowel diseases tested. These results are also consistent with the failure of others to isolate a virus by the techniques used. With the limited range of techniques used no evidence was obtained to implicate conventional viruses in the pathogenesis of Crohn's disease.

Full text

PDF
823

Images in this article

825Figure
on p.825

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aronson M. D., Phillips C. A., Beeken W. L., Forsyth B. R. Isolation and characterization of a viral agent from intestinal tissue of patients with Crohn's disease and other intestinal disorders. Prog Med Virol. 1975;21:165–176. [PubMed] [Google Scholar]
  2. Cave D. R., Mitchell D. N., Brooke B. N. Experimental animal studies of the etiology and pathogenesis of Crohn's disease. Gastroenterology. 1975 Sep;69(3):618–624. [PubMed] [Google Scholar]
  3. Cave D. R., Mitchell D. N., Brooke B. N. Induction of granulomas in mice by Crohn's disease tissue. Gastroenterology. 1978 Oct;75(4):632–637. [PubMed] [Google Scholar]
  4. Cave D. R., Mitchell D. N., Kane S. P., Brooke B. N. Further animal evidence of a transmissible agent in Crohn's disease. Lancet. 1973 Nov 17;2(7838):1120–1122. doi: 10.1016/s0140-6736(73)90936-7. [DOI] [PubMed] [Google Scholar]
  5. Das K. M., Valenzuela I., Morecki R. Crohn disease lymph node homogenates produce murine lymphoma in athymic mice. Proc Natl Acad Sci U S A. 1980 Jan;77(1):588–592. doi: 10.1073/pnas.77.1.588. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Emerit I., Emerit J., Levy A., Keck M. Chromosomal breakage in Crohn's disease: anticlastogenic effect of D-penicillamine and L-cysteine. Hum Genet. 1979;50(1):51–57. doi: 10.1007/BF00295589. [DOI] [PubMed] [Google Scholar]
  7. Gitnick G. L., Arthur M. H., Shibata I. Cultivation of viral agents from Crohn's disease. A new sensitive system. Lancet. 1976 Jul 31;2(7979):215–217. doi: 10.1016/s0140-6736(76)91022-9. [DOI] [PubMed] [Google Scholar]
  8. Gitnick G. L., Rosen V. J. Electron microscopic studies of viral agents in Crohn's disease. Lancet. 1976 Jul 31;2(7979):217–219. doi: 10.1016/s0140-6736(76)91023-0. [DOI] [PubMed] [Google Scholar]
  9. KYLE J., BELL T. M., PORTEOUS I. B., BLAIR D. W. FACTORS IN THE AETIOLOGY OF REGIONAL ENTERITIS. Bull Soc Int Chir. 1963 Nov-Dec;22:575–584. [PubMed] [Google Scholar]
  10. Mitchell D. N., Rees R. J. Agent transmissible from Crohn's disease tissue. Lancet. 1970 Jul 25;2(7665):168–171. doi: 10.1016/s0140-6736(70)92532-8. [DOI] [PubMed] [Google Scholar]
  11. Phillpotts R. J., Hermon-Taylor J., Brooke B. N. Virus isolation studies in Crohn's disease: a negative report. Gut. 1979 Dec;20(12):1057–1062. doi: 10.1136/gut.20.12.1057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SCHNEIERSON S. S., GARLOCK J. H., SHORE B., STUART W. D., STEINGLASS M., ARONSON B. Studies on the viral etiology of regional enteritis and ulcerative colitis: a negative report. Am J Dig Dis. 1962 Sep;7:839–843. doi: 10.1007/BF02231919. [DOI] [PubMed] [Google Scholar]
  13. Taub R. N., Sachar D., Janowitz H., Siltzbach L. E. Induction of granulomas in mice by inoculation of tissue homogenates from patients with inflammatory bowel disease and sarcoidosis. Ann N Y Acad Sci. 1976;278:560–564. doi: 10.1111/j.1749-6632.1976.tb47070.x. [DOI] [PubMed] [Google Scholar]
  14. Whorwell P. J., Phillips C. A., Beeken W. L., Little P. K., Roessner K. D. Isolation of reovirus-like agents from patients with Crohn's disease. Lancet. 1977 Jun 4;1(8023):1169–1171. doi: 10.1016/s0140-6736(77)92714-3. [DOI] [PubMed] [Google Scholar]

Articles from Gut are provided here courtesy of BMJ Publishing Group

RESOURCES