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. 1982 Sep;23(9):766–769. doi: 10.1136/gut.23.9.766

Enumeration of lymphocyte populations defined by surface markers in the whole blood of patients with Crohn's disease.

E O Pepys, E A Fagan, G A Tennent, V S Chadwick, M B Pepys
PMCID: PMC1419763  PMID: 6980813

Abstract

The proportions and absolute numbers of different lymphocyte populations were determined using alkaline phosphatase-labelled reagents in the whole peripheral blood of 22 patients with Crohn's disease. Monoclonal mouse anti-T cell antibody (OKT3) was used to identify T cells, polyvalent F(ab')2 anti-human immunoglobulin for B cells, C3b for C3b-receptor bearing cells, and soluble IgG antibody-antigen complexes for Fc(gamma)-receptor bearing cells. Endogenous myeloperoxidase served to distinguish monocytes. Application of this methodology to whole blood avoids the inevitable loss of cells which accompanies separation of mononuclear cells from blood and therefore permits precise enumeration of lymphocyte populations in the circulation. No significant difference from healthy adult controls was observed in any of the lymphocyte subsets tested.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aas J., Huizenga A., Newcomer A. D., Shorter R. G. Inflammatory bowel disease: lymphocytic responses to nonspecific stimulation in vitro. Scand J Gastroenterol. 1972;7(4):299–303. doi: 10.3109/00365527209180746. [DOI] [PubMed] [Google Scholar]
  2. Asquith P., Kraft S. C., Rothberg R. M. Lymphocyte responses to nonspecific mitogens in inflammatory bowel disease. Gastroenterology. 1973 Jul;65(1):1–7. [PubMed] [Google Scholar]
  3. Auer I. O., Götz S., Ziemer E., Malchow H., Ehms H. Immune status in Crohn's disease. 3. Peripheral blood B lymphocytes, enumerated by means of F(ab)2-antibody fragments, Null and T lymphocytes. Gut. 1979 Apr;20(4):261–268. doi: 10.1136/gut.20.4.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bach M. A., Bach J. F. The use of monoclonal anti-T cell antibodies to study T cell imbalances in human diseases. Clin Exp Immunol. 1981 Sep;45(3):449–456. [PMC free article] [PubMed] [Google Scholar]
  5. Best W. R., Becktel J. M., Singleton J. W. Rederived values of the eight coefficients of the Crohn's Disease Activity Index (CDAI). Gastroenterology. 1979 Oct;77(4 Pt 2):843–846. [PubMed] [Google Scholar]
  6. Binder H. J., Spiro H. M., Thayer W. R., Jr Delayed hypersensitivity in regional enteritis and ulcerative colitis. Am J Dig Dis. 1966 Jul;11(7):572–574. doi: 10.1007/BF02233570. [DOI] [PubMed] [Google Scholar]
  7. Bird A. G., Britton S. No evidence for decreased lymphocyte reactivity in Crohn's disease. Gastroenterology. 1974 Nov;67(5):926–932. [PubMed] [Google Scholar]
  8. Bolton P. M., James S. L., Newcombe R. G., Whitehead R. H., Hughes L. E. The immune competence of patients with inflammatory bowel disease. Gut. 1974 Mar;15(3):213–219. doi: 10.1136/gut.15.3.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Brown G., Greaves M. F. Enumeration of absolute numbers of T and B lymphocytes in human blood. Scand J Immunol. 1974;3(2):161–172. doi: 10.1111/j.1365-3083.1974.tb01244.x. [DOI] [PubMed] [Google Scholar]
  10. Druguet M., Pepys M. B. Enumeration of lymphocyte populations in whole peripheral blood with alkaline phosphatase-labelled reagents. A method for routine clinical use. Clin Exp Immunol. 1977 Jul;29(1):162–167. [PMC free article] [PubMed] [Google Scholar]
  11. Haegert D. G., Coombs R. R. Do human B and null lymphocytes form a single immunoglobulin-bearing population? Lancet. 1979 Nov 17;2(8151):1051–1053. doi: 10.1016/s0140-6736(79)92446-2. [DOI] [PubMed] [Google Scholar]
  12. Hodgson H. J., Wands J. R., Isselbacher K. J. Decreased suppressor cell activity in inflammatory bowel disease. Clin Exp Immunol. 1978 Jun;32(3):451–458. [PMC free article] [PubMed] [Google Scholar]
  13. Jones J. V., Housley J., Ashurst P. M., Hawkins C. F. Development of delayed hypersensitivity to dinitrochlorobenzene in patients with Crohn's disease. Gut. 1969 Jan;10(1):52–56. doi: 10.1136/gut.10.1.52. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LOCKHART-MUMMERY H. E., MORSON B. C. CROHN'S DISEASE OF THE LARGE INTESTINE. Gut. 1964 Dec;5:493–509. doi: 10.1136/gut.5.6.493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LOCKHART-MUMMERY H. E., MORSON B. C. Crohn's disease (regional enteritis) of the large intestine and its distinction from ulcerative colitis. Gut. 1960 Jun;1:87–105. doi: 10.1136/gut.1.2.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lyanga J. J., Davis P., Thomson A. B. In vitro testing of immunoresponsiveness in patients with inflammatory bowel disease: prevalence and relationship to disease activity immunoresponsiveness in IBD. Clin Exp Immunol. 1979 Jul;37(1):120–125. [PMC free article] [PubMed] [Google Scholar]
  17. Maclaurin B. P., Cooke W. T., Ling N. R. Impaired lymphocyte reactivity against tumour cells in patients with coeliac disease. Gut. 1971 Oct;12(10):794–800. doi: 10.1136/gut.12.10.794. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Meuwissen S. G., Schellekens P. T., Huismans L., Tytgat G. N. Impaired anamnestic cellular immune response in patients with Crohn's disease. Gut. 1975 Nov;16(11):854–860. doi: 10.1136/gut.16.11.854. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Meyers S., Sacher D. B., Taub R. N., Janowitz H. D. Anergy to dinitrochlorobenzene and depression of T-lymphocytes in Crohn's disease and ulcerative colitis. Gut. 1976 Nov;17(11):911–915. doi: 10.1136/gut.17.11.911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Morson B. C. Rectal biopsy in inflammatory bowel disease. N Engl J Med. 1972 Dec 28;287(26):1337–1339. doi: 10.1056/NEJM197212282872607. [DOI] [PubMed] [Google Scholar]
  21. PHEAR D. N. The relation between regional ileitis and sarcoidosis. Lancet. 1958 Dec 13;2(7059):1250–1251. doi: 10.1016/s0140-6736(58)91387-4. [DOI] [PubMed] [Google Scholar]
  22. Parent K., Barrett J., Wilson I. D. Investigation of the pathogenic mechanisms in regional enteritis with in vitro lymphocyte cultures. Gastroenterology. 1971 Oct;61(4):431–439. [PubMed] [Google Scholar]
  23. Pepys E. O., Pepys M. B. Enumeration in whole peripheral blood of lymphocytes bearing receptors for Fc(gamma) and C3b using alkaline phosphatase-labelled reagents. J Immunol Methods. 1980;32(4):305–314. doi: 10.1016/0022-1759(80)90023-x. [DOI] [PubMed] [Google Scholar]
  24. Pepys E. O., Tennent G. A., Pepys M. B. Enumeration of T and B lymphocytes in whole peripheral blood: absence of a null cell population. Clin Exp Immunol. 1981 Oct;46(1):229–234. [PMC free article] [PubMed] [Google Scholar]
  25. Pepys M. B., Dash A. C., Markham R. E., Thomas H. C., Williams B. D., Petrie A. Comparative clinical study of protein SAP (amyloid P component) and C-reactive protein in serum. Clin Exp Immunol. 1978 Apr;32(1):119–124. [PMC free article] [PubMed] [Google Scholar]
  26. Pepys M. B., Sategna-Guidetti C., Mirjah D. D., Wansbrough-Jones M. H., Dash A. C. Enumeration of immunoglobulin-bearing lymphocytes in whole peripheral blood. Clin Exp Immunol. 1976 Oct;26(1):91–94. [PMC free article] [PubMed] [Google Scholar]
  27. Reinherz E. L., Kung P. C., Goldstein G., Schlossman S. F. A monoclonal antibody with selective reactivity with functionally mature human thymocytes and all peripheral human T cells. J Immunol. 1979 Sep;123(3):1312–1317. [PubMed] [Google Scholar]
  28. Reinherz E. L., Moretta L., Roper M., Breard J. M., Mingari M. C., Cooper M. D., Schlossman S. F. Human T lymphocyte subpopulations defined by Fc receptors and monoclonal antibodies. A comparison. J Exp Med. 1980 Apr 1;151(4):969–974. doi: 10.1084/jem.151.4.969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Richens E. R., Williams M. J., Gough K. R., Ancill R. J. Mixed-lymphocyte reaction as a measure of immunological competence of lymphocytes from patients with Crohn's disease. Gut. 1974 Jan;15(1):24–28. doi: 10.1136/gut.15.1.24. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Röpke C. Lymphocyte transformation and delayed hypersensitivity in Crohn's disease. Scand J Gastroenterol. 1972;7(7):671–677. doi: 10.3109/00365527209180975. [DOI] [PubMed] [Google Scholar]
  31. Sachar D. B., Taub R. N., Brown S. M., Present D. H., Korelitz B. I., Janowitz H. D. Imparied lymphocyte responsiveness in inflammatory bowel disease. Gastroenterology. 1973 Feb;64(2):203–209. [PubMed] [Google Scholar]
  32. Sachar D. B., Taub R. N., Ramachandar K., Meyers S., Forman S. P., Douglas S. D., Janowitz H. D. T and B lymphocytes and cutaneous anergy in inflammatory bowel disease. Ann N Y Acad Sci. 1976;278:565–573. doi: 10.1111/j.1749-6632.1976.tb47071.x. [DOI] [PubMed] [Google Scholar]
  33. Strickland R. G., Korsmeyer S., Soltis R. D., Wilson I. D., Williams R. C., Jr Peripheral blood T and B cells in chronic inflammatory bowel disease. Gastroenterology. 1974 Oct;67(4):569–577. [PubMed] [Google Scholar]
  34. Thayer W. R., Jr, Charland C., Field C. E. The subpopulations of circulating white blood cells in inflammatory bowel disease. Gastroenterology. 1976 Sep;71(3):379–384. [PubMed] [Google Scholar]
  35. Victorino R. M., Hodgson H. J. Alteration in T lymphocyte subpopulations in inflammatory bowel disease. Clin Exp Immunol. 1980 Jul;41(1):156–165. [PMC free article] [PubMed] [Google Scholar]
  36. Walker J. G., Greaves M. F. Delayed hypersensitivity and lymphocyte transformation in Crohn's disease and proctocolitis. Gut. 1969 May;10(5):414–414. [PubMed] [Google Scholar]

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