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. 1984 Dec;25(12):1358–1363. doi: 10.1136/gut.25.12.1358

Ultrastructure of mouse intestinal mucosa and changes observed after long term anthraquinone administration.

P Dufour, P Gendre
PMCID: PMC1420212  PMID: 6510768

Abstract

In an attempt to study the relative toxicity of anthraquinonic laxatives on intestinal mucosa, we compared in mice the effects of fruit pulp containing sennosides A and B with those of a free anthraquinone, 1-8 dihydroxyanthraquinone. Observations have been made with transmission electron microscopy (EM) after 16 weeks of treatment with the two drugs. Although the doses used in this study were equipotent in terms of laxative activity, no damage to the intestinal tissue was observed with the sennosides. A number of changes, however, were detected in intestinal nervous tissues of all the animals treated with 1-8 dihydroxyanthraquinone, mainly in the form of vacuolisation of the axons, formation of lysosomal structures and in some cases appearances of fibrillar degeneration.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRITTAIN R. T., D'ARCY P. F., GRIMSHAW J. J. Observations on the use of a mouse bioassy method for investigating purgative activity. J Pharm Pharmacol. 1962 Nov;14:715–721. doi: 10.1111/j.2042-7158.1962.tb11165.x. [DOI] [PubMed] [Google Scholar]
  2. Breimer D. D., Baars A. J. Pharmacokinetics and metabolism of anthraquinone laxatives. Pharmacology. 1976;14 (Suppl 1):30–47. doi: 10.1159/000136684. [DOI] [PubMed] [Google Scholar]
  3. Dufour P., Gendre P., Meunier J. M., Canellas J. Tolérance de la muqueuse intestinale de la souris à l'ingestion prolongée d'une poudre de Séné. Ann Pharm Fr. 1983;41(6):571–578. [PubMed] [Google Scholar]
  4. Ewe K. The physiological basis of laxative action. Pharmacology. 1980;20 (Suppl 1):2–20. doi: 10.1159/000137392. [DOI] [PubMed] [Google Scholar]
  5. FAIRBAIRN J. W., SALEH M. R. I. Vegetable purgatives containing anthracene derivatives. VI. The instability of liquid preparations of senna during storage. J Pharm Pharmacol. 1953 May;5(5):317–326. [PubMed] [Google Scholar]
  6. Fairbairn J. W., Moss M. J. The relative purgative activities of 1,8-dihydroxyanthracene derivatives. J Pharm Pharmacol. 1970 Aug;22(8):584–593. doi: 10.1111/j.2042-7158.1970.tb10575.x. [DOI] [PubMed] [Google Scholar]
  7. Forth W., Rummel W., Baldauf J. Wasser- und Elektrolytbewegungam Dünn- und Dickdarm unter dem Einfluss von Laxantien, ein Beitrag zur Klärung ihresWirkungsmechanismus. Naunyn Schmiedebergs Arch Pharmakol Exp Pathol. 1966;254(1):18–32. [PubMed] [Google Scholar]
  8. Goerg K. J., Wanitschke R., Schulz L. Scanning electron microscopic study of the effect of rhein on the surface morphology of the rat colonic mucosa. Pharmacology. 1980;20 (Suppl 1):36–42. doi: 10.1159/000137396. [DOI] [PubMed] [Google Scholar]
  9. LOU T. C., FAIRBAIRN J. W. The biological assay of vegetable purgatives. J Pharm Pharmacol. 1951 Apr;3(4):225–232. doi: 10.1111/j.2042-7158.1951.tb13061.x. [DOI] [PubMed] [Google Scholar]
  10. Lemmens L., Borja E. The influence of dihydroxyanthracene derivatives on water and electrolyte movement in rat colon. J Pharm Pharmacol. 1976 Jun;28(6):498–501. doi: 10.1111/j.2042-7158.1976.tb02773.x. [DOI] [PubMed] [Google Scholar]
  11. Rask-Madsen J., Jensen P. B. Electrolyte transport capacity and electrical potentials of the normal and the inflamed human rectum in vivo. Scand J Gastroenterol. 1973;8(2):169–175. [PubMed] [Google Scholar]
  12. Rask-Madsen J. Transepithelial ionic transport and electrical polarization in the large bowel. Scand J Gastroenterol. 1974;9(3):223–229. [PubMed] [Google Scholar]
  13. Riemann J. F., Schenk J., Ehler R., Schmidt H., Koch H. Ultrastructural changes of colonic mucosa in patients with chronic laxative misuse. Acta Hepatogastroenterol (Stuttg) 1978 Jun;25(3):213–218. [PubMed] [Google Scholar]
  14. Sasaki K., Yamauchi K., Kuwano S. Metabolic activation of sennoside A in mice. Planta Med. 1979 Dec;37(4):370–378. doi: 10.1055/s-0028-1097352. [DOI] [PubMed] [Google Scholar]
  15. Smith B. Effect of irritant purgatives on the myenteric plexus in man and the mouse. Gut. 1968 Apr;9(2):139–143. doi: 10.1136/gut.9.2.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Smith B. Pathology of cathartic colon. Proc R Soc Med. 1972 Mar;65(3):288–288. [PMC free article] [PubMed] [Google Scholar]
  17. Thompson W. G. Laxatives: clinical pharmacology and rational use. Drugs. 1980 Jan;19(1):49–58. doi: 10.2165/00003495-198019010-00004. [DOI] [PubMed] [Google Scholar]

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